식물병연구 (Research in Plant Disease)

한국식물병리학회 (The Korean Society of Plant Pathology)
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Survival of Erwinia amylovora on Surfaces of Materials Used in Orchards

  • Choi, Hyun Ju (Applied Biology Program, Division of Bioresource Sciences, Kangwon National University) ;
  • Kim, Yeon Ju (Applied Biology Program, Division of Bioresource Sciences, Kangwon National University) ;
  • Lim, Yeon-Jeong (Applied Biology Program, Division of Bioresource Sciences, Kangwon National University) ;
  • Park, Duck Hwan (Applied Biology Program, Division of Bioresource Sciences, Kangwon National University)
  • 투고 : 2019.06.12
  • 심사 : 2019.06.16
  • 발행 : 2019.06.30
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초록

Fire blight disease caused by the bacterium, Erwinia amylovora, was observed in apple and pear orchards in Korea in 2015. Since then, it has spread, sometimes over long distances to other orchards. Therefore, we examined the ability of E. amylovora to survive in soils and on the surfaces of common materials such as T-shirts, wrist bands, pruning shears, and rubber boots by both conventional PCR (cPCR) and quantitative PCR (qPCR) methods. E. amylovora was detected in all materials tested in this study and survived for sufficiently long periods to cause fire blight disease in new sites. Thus, based on the results of this study, sanitation protocols must be applied to equipment during orchard work.

키워드

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Fig. 1. Bacterial population on MGY medium with rifampicin from suspension of soils (A), T-shirts, and wrist bands (B) and cotton balls rubbed on surface of pruning shears and rubber boots (C).

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Fig. 2. Bacterial detection on extracted DNAs in soils (A and B), and boiled suspensions of T-shirts (C), wrist bands (D), pruning shears (E) and rubber boots (F) using quantitative PCR (qPCR). E. amylovora cells were detected in all samples tested in this study based on earlier Ct values than 25 Ct mean as threshold.

Table 1. Bacterial detection on soils, T-shirts, wrist bands, pruning shears, and rubber boots using conventional PCR (cPCR)

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참고문헌

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