식물병연구 (Research in Plant Disease)

한국식물병리학회 (The Korean Society of Plant Pathology)
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Fusarium oxysporum에 의한 파프리카 잘록병

First Report of Fusarium oxysporum Causing Damping-off on Paprika in Korea

  • 박미정 (농촌진흥청 국립원예특작과학원 원예특작환경과) ;
  • 백창기 (농촌진흥청 국립원예특작과학원 원예특작환경과) ;
  • 서윤희 (농촌진흥청 국립원예특작과학원 원예특작환경과) ;
  • 박종한 (농촌진흥청 국립원예특작과학원 원예특작환경과)
  • Park, Mi-Jeong (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science) ;
  • Back, Chang-Gi (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science) ;
  • Seo, Yunhee (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science) ;
  • Park, Jong-Han (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science)
  • 투고 : 2019.04.03
  • 심사 : 2019.06.10
  • 발행 : 2019.06.30
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초록

육묘장에서 파프리카 유묘의 줄기와 뿌리가 갈변하고 잎이 시드는 증상이 나타났으며 병든 어린 식물체는 묘판에 서있지 못하고 쓰러졌다. 파프리카 줄기와 뿌리의 감염 부위에서 곰팡이 단포자 균주를 획득하였다. 분리 균주를 carnation leaf agar에 배양하여 균학적 특성을 관찰한 결과, 대형분생포자는 약간 굽은 낫 모양으로 무색이고 $18-26{\times}3-5{\mu}m$이며 1-3개의 격벽이 있었다. 소형분생포자는 타원형으로 무색이고 $6-10{\times}2-4{\mu}m$이며 격벽이 없었다. 후벽포자는 원형으로 $7-12{\mu}m$이며 균사의 끝이나 중간에 형성되었다. 균주의 포자현탁액을 파프리카 종자와 유묘에 접종하여 병원성을 검정한 결과, 이 병원균은 종자의 발아를 억제하는 영향을 주었으며 유묘에는 잘록병 증상이 나타났다. 병든 파프리카 유묘에서 분리한 균주의 형태적 특성을 비롯하여 TEF 및 RPB1 유전자 염기서열 분석을 통해 원인균을 Fusarium oxysporum으로 동정하였으며, 파프리카 잘록병으로 명명하여 국내 처음으로 보고하고자 한다.

In February 2019, a damping-off disease occurred at the seedling stage of paprika in a commercial nursery located in Cheorwon, Korea. A species of Fusarium was isolated from the diseased plant and it was identified as Fusarium oxysporum based on morphological characteristics and nucleotide sequence data of translation elongation factor $1-{\alpha}$ and the largest subunit of RNA polymerase. The isolate obtained was revealed to be pathogenic to the host plant through pathogenicity tests, and the reisolation of the pathogen confirmed Koch's postulates. This is the first report of damping-off caused by Fusarium oxysporum on paprika in Korea.

키워드

SMBRCU_2019_v25n2_94_f0001.png 이미지

Fig. 1. Characteristics of Fusarium oxysporum causing damping-off on seedlings of paprika.

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Fig. 2. Neighbor-joining tree based on the partial sequences of TEF (translation elongation factor 1-α) showing phylogenetic affinities of the one isolate of Fusarium oxysporum obtained in this study with isolates of other Fusarium spp. Fusarium solani was designated as the outgroup. Isolate in boldface was sequenced in this study. The scale bar represents 0.02 nucleotide substitutions per site.

SMBRCU_2019_v25n2_94_f0003.png 이미지

Fig. 3. Pathogenicity of Fusarium oxysporum on paprika seedlings.

Table 1. Pathogenicity test results on seeds and seedlings of two paprika cultivars

SMBRCU_2019_v25n2_94_t0001.png 이미지

참고문헌

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