Journal of Microbiology and Biotechnology

  • 제29권6호
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  • Pages.933-943
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  • 2019
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Gamma-Aminobutyric Acid Production from a Novel Enterococcus avium JS-N6B4 Strain Isolated from Edible Insects

  • Jo, Min-Ho (Department of Food Science Technology, Chonnam National University) ;
  • Hong, Seong-Jin (Department of Food Science Technology, Chonnam National University) ;
  • Lee, Ha-Nul (Department of Food Science Technology, Chonnam National University) ;
  • Ju, Jung-Hyun (Microbial Biotechnology Research Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Park, Bo-Ram (Department of Agro-food Resources National Academy of Agricultural Science, Rural Development Administration) ;
  • Lee, Jun-ho (Department of Biotechnology, Chonnam National University) ;
  • Kim, Sun-Am (Jeonnam Bioindustry Foundation Bio Control Research Center) ;
  • Eun, Jong-Bang (Department of Food Science Technology, Chonnam National University) ;
  • Wee, Young-Jung (Department of Food Science and Technology, Yeungnam University) ;
  • Kim, Young-Min (Department of Food Science Technology, Chonnam National University)
  • 투고 : 2019.05.02
  • 심사 : 2019.06.03
  • 발행 : 2019.06.28
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초록

Gamma-aminobutyric acid (GABA)-producing strains were isolated from four edible insects and subjected to 16S rRNA sequence analysis. Among the four GABA-producing bacteria, Enterococcus avium JS-N6B4 exhibited the highest GABA-production, while cultivation temperature, initial pH, aerobic condition, and mono-sodium glutamate (MSG) feeding were found to be the key factors affecting GABA production rate. The culture condition was optimized in terms of glucose, yeast extract, and MSG concentrations using response surface methodology (RSM). GABA production up to 16.64 g/l was obtained under the conditions of 7 g/l glucose, 45 g/l yeast extract, and 62 g/l MSG through the optimization of medium composition by RSM. Experimental GABA production was 13.68 g/l, which was close to the predicted value (16.64 g/l) calculated from the analysis of variance, and 2.79-fold higher than the production achieved with basic medium. Therefore, GABA-producing strains may help improve the GABA production in edible insects, and provide a new approach to the use of edible insects as effective food biomaterials.

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참고문헌

  1. Manyam BV, Katz L, Hare TA, Kaniefski K, Tremblay RD. 1981. Isoniazid-induced elevation of CSF GABA levels and effects on chorea in Huntington's disease. Ann. Neurol. 10: 35-37. https://doi.org/10.1002/ana.410100107
  2. Lim HS, Cha IT, Lee HJ, Seo MJ. 2016. Optimization of ${\gamma}$-aminobutyric acid production by Enterococcus faecium JK29 isolated from a traditional fermented foods. Microbiol. Biotechnol. Lett. 44: 26-33. https://doi.org/10.4014/mbl.1512.12004
  3. Tajabadi N, Ebrahimpour A, Baradaran A, Rahim RA, Mahyudin NA, Manap MY, et al. 2015. Optimization of ${\gamma}$-aminobutyric acid production by Lactobacillus plantarum Taj-Apis362 from honeybees. Molecules 20: 6654-6669. https://doi.org/10.3390/molecules20046654
  4. Wong CG, Bottiglieri T, Snead OC 3rd. 2003. GABA, ${\gamma}$-hydroxybutyric acid, and neurological disease. Ann. Neurol. 54: S3-S12.
  5. Inoue K, Shirai T, Ochial H, Kasao M, Hayakawa K, Kimura M, et al. 2003. Blood-pressure-lowering effect of a novel fermented milk containing ${\gamma}$-aminobutyric acid (GABA) in mild hypertensives. Eur. J. Clin. Nutr. 57: 490-495. https://doi.org/10.1038/sj.ejcn.1601555
  6. Jakobs C, Jaeken J, Gibson KM. 1993. Inherited disorders of GABA metabolism. J. Inherit. Metab. Dis. 16: 704-715. https://doi.org/10.1007/BF00711902
  7. Barragan A, Weidner JM, Jin Z, Korpi ER, Birnir B. 2015. GABAergic signaling in the immune system. Acta Physiol. 213: 819-827. https://doi.org/10.1111/apha.12467
  8. Seikuse T, Horino T, Mori Y. 1994. Accumulation of ${\gamma}$-aminobutyric acid (Gaba) in the rice germ during water soaking. Biosci. Biotechnol. Biochem. 58: 2291-2292. https://doi.org/10.1271/bbb.58.2291
  9. Pliner P, Salvy SJ. 2006. Food neophobia in human, pp. 75-92. In Shepherd R, Raats M (eds.), The Psychology of Food Choice. Oxfordshire, CABI Publishing.
  10. Huis A van, Itterbeeck JV, Llunder H, Mertens E, Halloran A, Muir G, et al. 2013. Edible insects: future prospects for food and feed security. Rome: Food and Agriculture Organization of the United Nations (FAO forestry paper 171). ISBN 9789251075968-187. Available from http://www.fao.org/3/i3253e/i3253e.pdf. Accessed Apr. 28, 2019.
  11. Lim HS, Cha IT, Rh SW, S h in HH, Seo MJ. 2017. Enhanced production of gamma-aminobutyric acid by optimizing culture conditions of Lactobacillus brevis HYE1 isolated from kimchi, a Korean fermented food. J. Microbiol. Biotechnol. 27: 450-459. https://doi.org/10.4014/jmb.1610.10008
  12. Lee KW, Shim JM, Yan Z, Kim JA, Kim JH. 2018. Properties of kimchi fermented with GABA-producing lactic acid bacteria as a starter. J. Microbiol. Biotechnol. 28: 209-221.
  13. Lee HJ, Son JY, Lee SJ, Lee HS, Kee BJ, Choi IS, et al. 2017. Production of gamma-aminobutyric acid (GABA) by Lactobacillus plantarum subsp. plantarum B-134 isolated from Makgeolli, traditional Korean rice wine. J. Life Sci. 27: 567-574. https://doi.org/10.5352/JLS.2017.27.5.567
  14. Vo T, Park JH. 2019. Characteristics of potential gammaaminobutyric acid-producing bacteria isolated from Korean and Vietnamese fermented fish products. J. Microbiol. Biotechnol. 29: 209-221. https://doi.org/10.4014/jmb.1811.09072
  15. Nomura M, Kimoto H, Someya Y, Furukawa S, Suzuki I. 1998. Production of gamma-aminobutyric acid by cheese starters during cheese ripening. J. Dairy Sci. 81: 1486-1491. https://doi.org/10.3168/jds.S0022-0302(98)75714-5
  16. Park KB, Oh SH. 2007. Production of yogurt with enhanced levels of gamma-aminobutyric acid and valuable nutrients using lactic acid bacteria and germinated soybean extract. Bioresour. Technol. 98: 1675-1679. https://doi.org/10.1016/j.biortech.2006.06.006
  17. Siragusa S, De Angelis M, Di Cagno R, Rizzello CG, Coda R, Gobbetti M. 2007. Synthesis of c-aminobutyric acid by lactic acid bacteria isolated from a variety of Italian cheeses. Appl. Environ. Microbiol. 73: 7283-7290. https://doi.org/10.1128/AEM.01064-07
  18. Rizzello CG, Cassone A, Di Cagno R, Gobbetti M. 2008. Synthesis of angiotensin I-converting enzyme (ACE)-inhibitory peptides and gamma-aminobutyric acid (GABA) during sourdough fermentation by selected lactic acid bacteria. J. Agric. Food Chem. 56: 6936-6943. https://doi.org/10.1021/jf800512u
  19. Li H, Cao Y , Gao D, Xu H. 2008. A high c-aminobutyric acid producing ability Lactobacillus brevis isolated from Chinese traditional paocai. Ann. Microbiol. 58: 649-653. https://doi.org/10.1007/BF03175570
  20. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. 1997. The CLUSTAL_X window interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 25: 4876-4882. https://doi.org/10.1093/nar/25.24.4876
  21. Li H, Qui T, Cao Y, Yang J, Huang Z. 2009. Pre-staining paper chromatography method for quantification of ${\gamma}$-aminobutyric acid. J. Choromatogr. A. 1216: 5057-5060. https://doi.org/10.1016/j.chroma.2009.04.044
  22. Su D, Robyt JF. 1993. Control of the synthesis of dextran and acceptor-products by Leuconostoc mesenteroides B-512FM dextransucrase. Carbohydr. Res. 248: 339-348. https://doi.org/10.1016/0008-6215(93)84139-W
  23. Rossetti V, Lombard A. 1996. Determination of glutamate decarboxylase by high-performance liquid chromatography. J. Chromatogr. B. Biomed. Appl. 681: 63-67. https://doi.org/10.1016/0378-4347(96)88202-8
  24. Li H, Cao Y. 2010. Lactic acid bacterial cell factories for gamma-aminobutyric acid. Amino Acids 39: 1107-1116. https://doi.org/10.1007/s00726-010-0582-7
  25. Kook MC, Cho SC. 2013. Production of GABA (gamma amino butyric acid) by lactic acid bacteria. Korean J. Food Sci. An. 33: 377-389. https://doi.org/10.5851/kosfa.2013.33.3.377
  26. Wang JJ, Lee CL, Pan TM. 2003. Improvement of monacolin K, c-aminobutyric acid and citrinin production ratio as a function of environmental conditions of Monascus purpureus NTU 601. J. Ind. Microbiol. Biotechnol. 30: 669-676. https://doi.org/10.1007/s10295-003-0097-2
  27. Kim JY, Lee MY, Ji GE, Lee YS, Hwang KT. 2009. Production of ${\gamma}$-aminobutyric acid in black raspberry juice during fermentation by Lactobacillus brevis GABA 100. Int. J. Food Microbiol. 130: 12-16. https://doi.org/10.1016/j.ijfoodmicro.2008.12.028
  28. Moldzio R, Radad K, Krewenka C, Kranner B, Duvigneau JC, Rausch WD. 2013. Protective effects of resveratrol on glutamate-induced damages in murine brain cultures. J. Neural. Transm (Vienna). 120: 1271-1280. https://doi.org/10.1007/s00702-013-1000-6
  29. Yang H, Xing R, Hu L, Liu S, Li P. 2016. Accumulation of ${\gamma}$-aminobutyric acid by Enterococcus avium 9184 in scallop solution in a two-stage fermentation strategy. Microb. Biotechnol. 9: 478-485. https://doi.org/10.1111/1751-7915.12301
  30. Shin NR, Moon JS, Shin SY, Li L, Lee YB, Kim TJ, et al. 2015. Isolation and characterization of human intestinal Enterococcus avium EFEL009 converting rutin to quercetin. Lett. Appl. Microbiol. 62: 68-74. https://doi.org/10.1111/lam.12512
  31. Gonzales RD, Schreckenberger PC, Graham MB, Kelkar S, DenBesten K, Quinn JP. 2001. Infections due to vancomycinresistant Enterococcus faecium resistant to linezolid. Lancet 357: 1179. https://doi.org/10.1016/S0140-6736(00)04376-2