Journal of the Korea Convergence Society (한국융합학회논문지)

Korea Convergence Society (한국융합학회)
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A Study on the Expression of Thymosin-β4 and c-Myc mRNA in the Model of liver cirrhosis with fibrosis

섬유화 진행 간경변 조직 모델에서 Thymosin β4와 C-myc mRNA 융합 발현 연구

  • Kim, Jean-Soo (Department of Clinical Laboratory Science, Daejeon Health Institute of Technology) ;
  • Park, Un-Kyu (Department of Clinical Laboratory Science, Daejeon university)
  • 김진수 (대전보건대학교 임상병리과) ;
  • 박운규 (대전대학교 임상병리과)
  • Received : 2019.03.28
  • Accepted : 2019.06.20
  • Published : 2019.06.28
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Abstract

The propose of this study has been conducted to examine expression of c-Myc and Thymosin-${\beta}4$ in liver cirrhosis model from liver fibrosis and For the method of study, the experiment was conducted in 2 groups; liver cirrhosis model experiment group due to liver fibrosis and control group with distilled water. This study outcome showed that liver cirrhosis model experiment group had significantly higher expression of c-Myc and Thymosin-${\beta}4$. with changes to hepatic tissue of special staining and electron microscopy. In conclusion, in clinical tests regarding liver function, molecular evaluation of c-Myc and Thymosin-${\beta}4$ and their expression along with serological change and histological assessment can be utilized as a reference for diagnosing liver disease for prevention and diagnosis of the disease, Based on this research in the future, we will carry out an in-depth study by adding the types of experimental groups and related genes.

이 연구의 목적은 간 섬유화로 인한 간경변 모델에서 c-Myc과, Thymosin-${\beta}4$ 유전자의 발현을 알아 보고자 실시하였으며 연구 방법은 2군으로 나누어 섬유화로 인한 간경변 모델 실험군과 증류수를 이용한 대조군으로 분류 한 후 실험을 하였다. 본 연구를 통한 결과는 간경변 모델 실험군은 대조군에 비하여 c-Myc과 Thymosin-${\beta}4$의 발현이 증가 되는 것을 뚜렷하게 알 수 있었고, 전자현미경적 검경 외에 여러 특수 염색을 통하여 간조직의 변화를 관찰 할 수 있었다. 결론적으로 기존 간기능 검사 관련 임상검사에서 질환 예방과 질환 판정 시 혈청학적 변화와 조직학적인 검사와 더불어 c-Myc과 Thymosin-${\beta}4$의 분자 기법을 통한 유전자의 발현 상태를 확인함으로써 간 질환 판정에 기여 할 수 있을 것으로 사료 되며, 향후 이 연구를 기반으로 실험군의 종류와 관련 유전자를 추가 하여 심도 있는 연구를 진행할 예정이다.

Keywords

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Fig. 1. Shapes of liver tissue in the TransmissionElectron Microscope.

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Fig. 2. H & E stain of liver tissue.

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Fig .3. Masson's Trichrom stain of liver tissue.

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Fig. 4. Reticular fiber stain of liver tissue.

Table 1. Method of Hematoxylin & Eosin stain

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Table 2. Transmission Electron Microscope : Method of TEM stain

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Table 3. Method of Masson's Trichrom stain

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Table 4. Method of Gomori Reticulum stain

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Table 5. Primers used for the detection of gene expression

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Table 6. Relative expression of β-actin, c-Myc and Thymosin-β4 gene expression

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Table 7. Cq values of gene expression in groups administered with CCl4

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