• Journal of the Korea Academia-Industrial cooperation Society
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• v.12 no.7
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• pp.3310-3316
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• 2011

Hematoxylin is the main component of Hematoxylon campechianum which has been utilized in the southern provinces of Korea as a folk remedy for diabetic complications. In the present study, to investigate the hypoglycemic mechanism of hematoxylin, the 2-deoxyglucose uptake and phospholipid metabolism were examined in sciatic nerves from three groups of rats : normal control, diabetic control, diabetic hematoxylin-treated group. Hematoxylin significantly reduced blood glucose levels in diabetic control rats. On a wet weight basis, the nerves from diabetic rats showed a 20% decrease in total phospholipid from that of controls and a relative decrease in phosphatidylinositide. Hematoxylin treatment increased the incorporation rate of 2-[3H] myo-inositol into total phosphoinositids in diabetic rat. The effectiveness were more potent in higher dose hematoxylin-treated rats than lower dose hematoxylin-treated rats. These results suggest that hematoxylin increases glucose transport and lipid metabolism by partially normalizing concerned with myo-inositol metabolism in diabetic rat. Therefore we propose that hematoxylin can be a promising candidate for diabetes medication.

• Journal of Food Hygiene and Safety
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• v.2 no.1
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• pp.35-40
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• 1987

The antilipidperoxidative effects of Brazilin and Hematoxylin were investigated at the levels of liver~total homogenates, -microsomal fraction, -mitochondrial fraction and the sera of SD-rats intoxicated with $CCl_4$ and ethanol. Both natural dyes markedly inhibited the lipidperoxidation induced by $CCl_4$ and ethanol. Brazilin and Hematoxylin showed the inhibitory effects on the both enzymatic (NADPH-dependent) and nonenzymatic (Ascorbate-induced) lipidperoxidation pathways. but it is supposed that the antilipidperoxidative powers of them mainly result from the inhibition of the nonenzymatic lipidperoxidation.

• Toxicological Research
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• v.4 no.2
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• pp.151-158
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• 1988

Experiments were undertaken to evaluate the effects of brazilin and hematoxylin on immune functions in normal young mice. Brazilin and hematoxylin decreased the circulating leucocyte counts. Brazilin and hematoxylin decreased the cirulating leucocyte counts. Both compounds did not produce a significant change of immunoorgan weights in comparison with those in the control group. Brazilin decreased significantly IgM plaque forming cells. Brazilin and hematoxylin decreased significantly Arthus reaction at 3 hours-post challenge with respect to aggregated bovine serum albumin.

• Journal of Food Hygiene and Safety
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• v.7 no.2
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• pp.77-82
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• 1992

In order to investigate the protein binding characteristics of braD6n and hematoxy6n to bovine semm albumin (BSA), the fluorescence probe method was adopted. Brazilin and hematoxy6n showed strong binding affinity for BSA. It was also confirmed that hematoxy6n was bound to BSA stronger than braDlin. The association constants were decreased by the elevation of concentrations of brazilin and hematoxylin. It might be due to the complex formation of the probe and both compounds or the interaction between the probe-protein complex and both compounds. The bindings between both compounds and BSA were dependent on pH and ionic strength. It seems that electrostatic force as weD as hydrophobic force is involved in the binding of braD6n and bematoxylin to BSA.

• Journal of Food Hygiene and Safety
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• v.7 no.1
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• pp.53-58
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• 1992

Hypoglycemic effect of hematoxylin was observed in streptozotocin-induced diabetic rats, of which plasma insulin levels were not affected. Investigation of hypoglycemic mechanism showed that hematoxylin stimulated glucose oxidation and glycogen synthesis in soleus muscle from diabetic rats in both basal and insulin stimulated state.

• Biomedical Science Letters
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• v.10 no.3
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• pp.259-262
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• 2004

The aim of present study was to examine the effect of carrageenan on the tendon of skeletal muscle of rat. The tendon damage was induced by injection of carrageenan into skeletal hind muscle of rats. Rats were killed on 48 hours after carrageenan injection. The resulting tendons were fixed with 10％ neutral buffered formalin (NBF), dehydrated, embedded, sectioned by 4 μm, and stained by phosphotungstic acid hematoxylin (PTAH) or hematoxylin-eosin (H-E). Carrageenan induced the segregation of tendon fibers, intratendinous cleft, segregation of muscle cell group, wave arrangement of tendon fiber. The results suggest that carrageenan induced tendon damage of rat's skeletal muscle by morphological changes.

• Archives of Pharmacal Research
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• v.11 no.2
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• pp.145-148
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• 1988

Effects of brazilin on the blood viscosity and erythrocyte deformability were investigated in alloxan induced diabetic rats. By the treatment to alloxan induced diabetic rats, enhancement of erythrocyte deformability was observed. In the in vitro study on the erythrocyte deformability, brazilin showed statistically significant improving effects on the erythrocyte deformability. At the concentrations of $10^3$ M of brazilin, erythrocyte deformability was compared with those of hematoxylin, pentoxifyline and prednisolone.

• Biomedical Science Letters
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• v.28 no.4
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• pp.290-297
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• 2022

Through previous studies, the central nervous system (CNS) was collected by dividing the scuttle fly into larval, pupa, and adult stages by developmental stage, and the morphological characteristics were observed. In situ hybridization (ISH) using the collected central nervous system, it was possible to confirm the location and extent of expression of the neurotransmitter corazonin (Crz) at each stage of development. In this study, paraffin specimens were prepared using central nervous system tissues of 3rd instar larval stage of scuttle fly, which had completed in situ hybridization, and general histochemical staining (hematoxylin-eosin, H-E) and special histochemical staining (luxol fast blue-cresyl violet) was performed to observe the histological and cytological morphology characteristics of corazonin neurons. As a result, a variety of nerve cell body existed between many myelin sheath. The corazonin neurons compose cortex of central nervous system with other neurons congregating in this tissue and show larger cell body relatively in neurohistochemical analysis.

• Journal of Radiation Protection and Research
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• v.25 no.4
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• pp.217-221
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• 2000

Radiotheraphy may be considered as one of the most effective treatments for digestive tumors. This procedure has major side effects, especially in fast growing tissues like intestinal mucosa. For this reason, the morphological changes in mouse jejunal villi after whole body ${\gamma}-irradiation$ were analysed. Four week old ICR male mice were irradiatied with 6.5Gy or 12Gy of whole body ${\gamma}-irradiation$ and were sacrificed 3 days later. Jejunum among intestine were taken for morphology. Samples were carried out dehydration process and sliced sample covered with paraffin was hold on the slide and then light microscopy was observed by Hematoxylin & Eosin staining. villi at both irradiated doses were showed that the length of villi were shortened and thickened and that lumen were expanded in comparison with non-irradiated group. Since willi have an important role in digestion and very sensitive to radiation from this test, it has a role of test parameter for finding radioprotectors as well as evaluating the biological effect by radiaton.

• The Journal of the Korea Contents Association
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• v.11 no.10
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• pp.286-292
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• 2011

This study investigated the degree of fracture healing using cathode stimulation of microcurrent, cathode and anode stimulation of High Voltage Pulsed Galvanic Current (HVPGC). Measures were performed by X-ray test and Hematoxylin-Eosin stain and Masson's trichrome stain and osteocalcin-positive immunoreactivity. In the measure of X-ray, microcurrent stimulation group revealed more rapid recovery than the groups of HVPGC's cathode and anode stimulation in bone union degrees. Microcurrent group showed significant difference statistically (p<0.05). However, the groups of HVPGC's cathode and anode stimulation didn't show significant difference statistically(p>0.05). In the histologic examination with Hematoxylin-Eosin and Masson's trichrome, microcurrent stimulation group was observed more proliferation of irregular woven bones than the groups of HVPGC's cathode and anode stimulation. Osteocalcin-positive immunoreactivity was observed more osteoblast, osteocyte, osteoclast, bone matrix than the groups of HVPGC's cathode and anode stimulation. Microcurrent stimulation can be considered an effective way during healing of fresh fracture and it can show more effective method than HVPGC's cathode and anode stimulation in the fracture healing.