Journal of Marine Bioscience and Biotechnology (한국해양바이오학회지)

The Korean Society for Marine Biotechnology (한국해양바이오학회)
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Isolation and Characterization of Five Isolates of Tetraselmis sp. with Rapid Growth Rates in Low Temperatures

저온 생장성이 우수한 분리 미세조류 Tetraselmis sp. 5개주의 생장 패턴 및 지방산 조성 분석

  • 박한울 (해양바이오에너지 생산기술개발연구센터, 인하대학교) ;
  • 허동희 (해양바이오에너지 생산기술개발연구센터, 인하대학교) ;
  • 신동우 (해양바이오에너지 생산기술개발연구센터, 인하대학교) ;
  • 김지훈 (국립낙동강생물자원관) ;
  • 홍성주 (해양바이오에너지 생산기술개발연구센터, 인하대학교) ;
  • 임상민 (해양바이오에너지 생산기술개발연구센터, 인하대학교) ;
  • 이철균 (해양바이오에너지 생산기술개발연구센터, 인하대학교)
  • Received : 2019.05.30
  • Accepted : 2019.06.18
  • Published : 2019.06.30
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Abstract

For successful microalgal biodiesel production, the strain should be selected carefully. Fast growth rate and high fatty acid contents are desired traits for algal biodiesel production. In ocean cultivation of microalgae, seawater temperature slowly changes over seasons, and rotating algal strains in accordance with their optimal temperature could improve overall productivity. Additionally, use of indigenous strain is preferred to alleviate potential impacts on the environment. In this study, five strains of Tetraselmis sp. from nearshore of Youngheung Island, Incheon, Korea, were isolated during winter and characterized for their growth patterns and fatty acid compositions in the low temperatures ($5-15^{\circ}C$). The five strains showed various characteristics in optimal growth temperature, fatty acid contents, and compositions. Compared with a strain of Tetraselmis sp., isolated from Ganghwa island in a previous study, a rapid-growing strain with 237% higher biomass productivity and an oleaginous strain with twice higher fatty acid contents at $10^{\circ}C$ were isolated. The oleaginous Tetraselmis strain showed the highest fatty acid productivity among the strains, having 438% higher productivity than the previous strain. Using the new isolates in the seasons with low seawater temperature would improve microalgal fatty acid productivity in ocean cultivation.

Keywords

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Figure 1. Phylogenetic Analysis of the 18S rDNA of New Tetraselmis sp. Isolates.

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Figure 2. Time Profiles of Biomass Concentrations of Tetraselmis sp. Strains at (a) 5°C, (b) 10°C, and (c) 15°C.

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Figure 3. Biomass Productivities by the Microalgal Strains and Temperature.

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Figure 4. Fatty Acid Contents of the Microalgae at 10°C.

Table 1. Sequences of Primers Used for Amplification of 18S rDNA

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Table 2. Fatty Acid Compositions of the Microalgae.

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