Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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The Disease Severity and Related Pathogens Caused by Root Rot on 6 Years Old Ginseng Cultivation Fields

인삼 6 년근 수확지의 뿌리썩음병 발생현황 및 관련 병원균 동정

  • Seo, Mun Won (Ginseng Research Division, Department of Herbal Crop Reserch, National Institute of Horticultural and Herbal Science, RDA) ;
  • Han, You Kyoung (Ginseng Research Division, Department of Herbal Crop Reserch, National Institute of Horticultural and Herbal Science, RDA) ;
  • Bae, Yeoung Seuk (Ginseng Research Division, Department of Herbal Crop Reserch, National Institute of Horticultural and Herbal Science, RDA) ;
  • Lee, Seung Ho (Ginseng Research Division, Department of Herbal Crop Reserch, National Institute of Horticultural and Herbal Science, RDA)
  • 서문원 (농촌진흥청 국립원예특작과학원 인삼특작부 인삼과) ;
  • 한유경 (농촌진흥청 국립원예특작과학원 인삼특작부 인삼과) ;
  • 배영석 (농촌진흥청 국립원예특작과학원 인삼특작부 인삼과) ;
  • 이승호 (농촌진흥청 국립원예특작과학원 인삼특작부 인삼과)
  • Received : 2018.11.21
  • Accepted : 2019.03.27
  • Published : 2019.04.30
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Abstract

Ginseng (Pnanx ginseng C. A. Meyer) is famous worldwide, and is very important cash crop and medicinal herb in Korea. It takes four to five years to produce harvestable ginseng roots, and ginseng is attacked by several pathogens during cultivation. We investigated the disease rate caused by ginseng root rot from 6 years old ginseng cultivation fields (Chungnam; 9 fields, Chungbuk; 11 fields, Gangwon 5 fields). The highest disease severity was Dangjin D (2.9) and the lowest one was Gaesan C (0.6). Of the 625 isolations, 340 isolations were classified as Ilyonectria radicicola and Fusarium solani. Finally, genetic diversity of I. radicicola and F. solani was confirmed by sequence analysis. Among the I. radicicola group, I. mors-panacis, which is known as highly virulent pathogen, and I. liriodendri, I. robusta and I. cyclamicicola, which are weakly virulent pathogens, were identified. In the case of F. solani, it is divided into two groups, but it is necessary to conduct diversity research through genetic analysis and pathogenetic studies using various markers. Based on these results, it could be used as a basic data for control of ginseng root rot pathogens.

인삼은 현금작물로 통용되는 약초로 국내에서 매우 중요한 작물 중 하나이다. 인삼의 수확까지 경작 기간은 4~5년으로 이 기간 동안 여러 토양병원균으로부터 피해를 받게되며, 심할 경우에는 폐포로 경제적 손실을 입게된다. 이러한 뿌리썩음병에 의한 피해 현황을 조사하기 위해 충남, 충북, 강원지역의 6 년근 채굴지 25농가를 대상으로 인삼 뿌리썩음병 발생현황, 발병지수 및 병원균을 분석하였다. 당진 D는 발병도가 2.9로 조사지역 중 가장 높았으며, 괴산 C는 가장 낮은 발병도를 보였다. 인삼 뿌리썩음병의 병반으로부터 병원균을 분리한 결과, 625 균주 중 54.4%가 인삼 뿌리썩음병 관련 병원균인 I. radicicola와 F. solani로 분류가 되었으며, 염기서열을 이용하여 이들 병원균의 유전적 다양성을 확인하였다. 특히 I. radicicola 그룹 내에서도 강병원성 균주로 알려진 I. mors-panacis와 상대적으로 병원성이 약한 I. liriodendri, I. robusta, I. cyclaminicola 등이 동정되었다. F. solani의 경우는 두 개의 그룹으로 구분되어 지나, 추후 다양한 마커를 이용한 유전분석과 병원성 연구를 통해 다양성 연구를 진행해야할필요가 있다. 추후 이 결과는 인삼의 뿌리썩음병 진단 및 방제 있어서 기초자료로 활용될 것으로 판단된다.

Keywords

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Fig. 1. The occurrence status and the disease index of root rot of 6 years old ginseng harvest fields. Ginseng for the investigation of root rot after harvest (A; yellow bar = 180 cm) and groups according to degree of root rot (B).

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Fig. 2. The ginseng with root rot disease and isolation of colony using PDA media. Ginseng root rot (A), isolation of pathogens from leasins (B; front C; reverse).

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Fig. 3. Phylogenetic analysis of Ilyonectria radicicola complex isolated from root rot of 6 years old ginseng using ITS region. The unidentified isolates of root rot of 6 years old ginsengs were in the same group as the existing I. radicicola complex. The bootstrap analysis was performed with 1,000 replications. Bold bar is I. radicicola complex.

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Fig. 4. Phylogenetic analysis of Fusarium solani isolated from root rot of 6 years old ginseng using ITS region. The unidentified isolates of root rot of 6 years old ginsengs were in the same group as the existing F. solani. The bootstrap analysis was performed with 1,000 replications. Bold bar is F. solani group.

Table 1. The disease severity from root rot of 6 years old ginseng on harvest fields

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Table 2. The pathogens isolated from root rot of 6 years old ginseng on harvest fields

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