Browse > Article
http://dx.doi.org/10.7732/kjpr.2019.32.2.144

The Disease Severity and Related Pathogens Caused by Root Rot on 6 Years Old Ginseng Cultivation Fields  

Seo, Mun Won (Ginseng Research Division, Department of Herbal Crop Reserch, National Institute of Horticultural and Herbal Science, RDA)
Han, You Kyoung (Ginseng Research Division, Department of Herbal Crop Reserch, National Institute of Horticultural and Herbal Science, RDA)
Bae, Yeoung Seuk (Ginseng Research Division, Department of Herbal Crop Reserch, National Institute of Horticultural and Herbal Science, RDA)
Lee, Seung Ho (Ginseng Research Division, Department of Herbal Crop Reserch, National Institute of Horticultural and Herbal Science, RDA)
Publication Information
Korean Journal of Plant Resources / v.32, no.2, 2019 , pp. 144-152 More about this Journal
Abstract
Ginseng (Pnanx ginseng C. A. Meyer) is famous worldwide, and is very important cash crop and medicinal herb in Korea. It takes four to five years to produce harvestable ginseng roots, and ginseng is attacked by several pathogens during cultivation. We investigated the disease rate caused by ginseng root rot from 6 years old ginseng cultivation fields (Chungnam; 9 fields, Chungbuk; 11 fields, Gangwon 5 fields). The highest disease severity was Dangjin D (2.9) and the lowest one was Gaesan C (0.6). Of the 625 isolations, 340 isolations were classified as Ilyonectria radicicola and Fusarium solani. Finally, genetic diversity of I. radicicola and F. solani was confirmed by sequence analysis. Among the I. radicicola group, I. mors-panacis, which is known as highly virulent pathogen, and I. liriodendri, I. robusta and I. cyclamicicola, which are weakly virulent pathogens, were identified. In the case of F. solani, it is divided into two groups, but it is necessary to conduct diversity research through genetic analysis and pathogenetic studies using various markers. Based on these results, it could be used as a basic data for control of ginseng root rot pathogens.
Keywords
Disease severity; Fusarium solani; Ginseng; Ilyonectria radicicola; Root rot;
Citations & Related Records
Times Cited By KSCI : 8  (Citation Analysis)
연도 인용수 순위
1 Reeleder, R.D. and R.A. Brammall. 1994. Pathogenicity of Pythium species, Cylindrocarpon destructans, and Rhizoctonia solani to ginseng seedlings in Ontario. Canadian Journal of Plant Pathology 16(4):311-316.   DOI
2 Reeleder, R.D., R. Roy and B. Capell. 2002. Seed and root rots of ginseng (Panax quinquefolius L) caused Cylindrocarpon destructans and Fusarium spp. Journal of Ginseng Research 26(3):151-158.   DOI
3 Seo, M.W. 2009. Analysis of characteristics for Korean ginseng root rot pathogen (Cylindrocarpon destructans) and development of new diagnostic molecular marker. Department of Agricultural Biology, Master Thesis, Chungnam National Univ., Korea. pp. 28-35.
4 Seo, M.W. 2017a. Reclassification and disease occurrence factor in first plantd field of Korean ginseng root rot pathogen population (Cylindrocarpon destructans) and practical disease diagnosis. Department of Agricultural Biology, Ph.D. Thesis, Chungnam National Univ., Korea. pp. 28-34.
5 Park, G.J. and H.S. Chung. 1997. Characteristics of soil groups based on the development of root rot of ginseng seedlings. Korean J. Plant Pathol. 13:46-56.
6 Seo, M.W., J.Y. Song, K.H. Kang, S.Y. Park, S.I. Kim and H.G. Kim. 2017b. Virulence assays and genetic reclassification to assess the pathogenicity of Cylindrocarpon destructans isolated from peony in ginseng. The Korean Journal of Mycology 45(2):132-138.   DOI
7 Seo, M.W., J.Y. Song, S.I. Kim, S.K. Oh and H.G. Kim. 2018. Pathogenicity on ginseng and sequence assys of Ilyonectria radicicola isolated from chestnut rhizosphere soils. Korean J. Medicinal Crop Sci. 26(4):302-307.   DOI
8 Shin, J.H., B.D. Yon, H.J. Kim, S.J. Kim and D.Y. Chung. 2012. Soil environment and soil-borne plant pathogen causing root rot disease of ginseng. Korean J. Soil Sci. Fert. 45(3):370-376.   DOI
9 Singleton, L.L., J.D. Mihail and C.M. Rush. 1992. Methods for research on soilborne phytopathogenic fungi: In Jeanne, M. et al., ASP Press. St. Paul. MN (USA). pp. 140-141.
10 Suga, H., T. Hasegawa, H. Mitsui, K. Kageyama and M. Hyakumachi. 2000. Phylogenetic analysis of the phytopathogenic fungus Fusarium solani based on the rDNA-ITS region. Mycol. Res. 104(10):1175-1183.   DOI
11 Cho, D.H., K.J. Park, Y.H. Yu, S.H. Ohh and H.S. Lee. 1995. Root-rot development of 2-year old ginseng (Panax ginseng C. A. Meyer) caused by Cylindrocarpon destructans (Zinssm) Scholten in the continuous cultivation field. Korean Journal of Ginseng Science 19:175-180.
12 Tamura, K., G. Stecher, D. Peterson, A. Filipski and S. Kumar. 2013. MEGA6: Molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol. 30(12):2725-2729.   DOI
13 The Korean Society of Plant Pathology (KSPP). 2009. List of plant disease in Korea. The Korean Society of Plant Pathology. Seoul, Korea. pp. 91, 284, 288.
14 Yu, Y.H., D.H. Cho, I.H. Lee and S.H. Ohh. 1990. Effect of seedling depth on severity of damping-off ginseng sedlings caused by Rhizoctonia solani. Korean J. Ginseng Sci. 14(3):432-436.
15 Ahn, C.H. 2017. Effect of fine bubble treatment on the growth of two-year-old ginseng. Korean J. Plant Res. 30(5):549-559.   DOI
16 Cabral, A., J.Z. Groenewald, C. Rego, H. Oliveira and P.W. Crous. 2012. Cylindrocarpon root rot: Multi-gene analysis reveals novel species within the Ilyonectria radicicola species complex. Mycol. Progress. 11(3):655-688.   DOI
17 Chung, H.S. 1972. Ginseng disease in Korea. Korea Pharmacognosy 2:73-79.
18 Doyle, J.J. 1990. Isolation of plant DNA from fresh tissue. Focus 12:13-15.
19 Kim, J.H. and M.W. Lee. 1974. On the root rot of ginseng (I) -Isolation and identification of Fusarium spp. Korean Journal of Microbiology 12:94-98.
20 Kang, S.W., B.Y. Yeon, G.S. Hyeon, Y.S. Bae, S.W. Lee and N.S. Seong. 2007. Changes of soil chemical properties and root injury ratio by progress years of post-harvest in continuous cropping soils of ginseng. Korean J. Medicinal Crop Sci. 15(3):157-161.
21 Kim, Y.H., J.H. Lee, S.H. Ohh, Y.H. Yu and I.H. Lee. 1993. Ginseng growths in abolished ginseng fields and factors affecting the ginseng growth. Korean J. Ginseng Sci. 17(1):45-51.
22 Lee, S.G. 2004. Fusarium species associated with ginseng (Panax ginseng) and their role in the root-rot of ginseng plant. Res. Plant Dis. 10(4):248-259.   DOI
23 Lee, S.W., S.H. Lee, M.W. Seo, I.B. Jang, I.B. Jang, J. You, J.W. Moon and S.J. Seo. 2018. Effect of soil fumigation and maize cultivation on reduction of replant failure in ginseng. Korean J. Medicinal Crop Sci. 26(3):248-253.   DOI
24 Matuo, T. and W.C. Snyder. 1972. Host virulence and the hypomyces stage of Fusarium solani f. sp. pisi. Phytopathology 62:731-735.   DOI
25 Matuo, T. and W.C. Snyder. 1973. Use of morphology and mating populations in the identification of formae speciales in Fusarium solani. Phytopathology 63:562-565.   DOI
26 Park, H.W., T.G. Lim, C.H. Choi and J.E. Choi. 2006. Factors and cause of rusty-ginseng occurrence. Korean J. Crop Sci. 51:396-400.
27 Punja, J.K. 1997. Fungal pathogens of American ginseng (Panax quinquefolius) in British Columbia. Canadian Journal of Plant Pathology 19(3):301-306.   DOI