The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Spore Diversity of Arbuscular Mycorrhizal Fungi in Upo Wetland

우포 습지에 분포하는 수지상균근균 포자의 다양성

  • Ko, Kang-Moon (Department of Biology Education, Korea National University of Education) ;
  • Park, Hyeok (Department of Biology Education, Korea National University of Education) ;
  • Ka, Kang-Hyeon (Special Forest Products Division, National Institute of Forest Science) ;
  • Eom, Ahn-Heum (Department of Biology Education, Korea National University of Education)
  • 고강문 (한국교원대학교 생물교육과) ;
  • 박혁 (한국교원대학교 생물교육과) ;
  • 가강현 (국립산림과학원 산림소득자원연구과) ;
  • 엄안흠 (한국교원대학교 생물교육과)
  • Received : 2018.08.09
  • Accepted : 2018.08.19
  • Published : 2019.03.01
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Abstract

We extracted arbuscular mycorrhizal fungal (AMF) spores from rhizospheres of three plants from Upo Wetland, Korea. We identified the isolated AMF spores based on morphological characteristics and phylogenetic analysis of partial 18S rDNA nucleotide sequences. The species diversity of AMF spores was calculated among the study sites and host plants. Consequently, nine species from six genera of AMF spores were identified. We confirmed the species diversity of the AMF spores in rhizospheres affected by host plants in the wetland. In the course of this study, we confirmed a previously unreported AMF species in Korea: Diversispora epigaea. We described the morphological features and molecular characteristics of this previously unreported AMF species.

우포 습지에 서식하는 식물의 근권 토양에서 수지상균근균(AMF) 포자를 추출하였다. 추출한 포자는 형태적 특성과 18S rDNA 염기서열의 분자적 분석을 이용해 동정하였다. 그 결과 6속 9종의 AMF 포자가 분리된 것을 확인하였으며, 종 다양성 지수를 계산하여 분석한 결과 습지에서 AMF 포자의 종 다양성은 식물에 의해 영향을 받는 것을 확인하였다. 연구 과정에서 국내 미기록종 1종을 확인하였으며, 확인된 종은 Diversispora epigaea이다. 미기록종 AMF 포자의 형태적 특성 및 분자적 분석의 결과에 대해 서술하였다.

Keywords

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Fig. 1. The information about sampling sites based on GPS coordinates. The dotted marks indicate sampling sites.

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Fig. 2. Neighbor-joining phylogenetic tree based on 18S partial rDNA sequences. Rhodotorula hordea was used as an outgroup. Numbers on branches indicate bootstrap values (1,000 replicates). Fungal strain isolated in this study is in bold.

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Fig. 3. Spores of Diversispora epigaea isolated from rhizosphere. Whole spores (A), spore mounted in PVLG (B, C), characteristics of spore walls (D, E), subtending hyphae (F) (scale bars =100 μm).

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Fig. 4. Neighbor-joining phylogenetic tree based on sequences of Krüger fragment. Acaulospora delicata was used as an outgroup. Numbers on branches indicate bootstrap values (1,000 replicates). Fungal strain isolated in this study is in bold.

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Fig. 5. Non-metric multidimensional scaling (NMS) plots for the spore communities of arbuscular mycorrhizal fungi isolated from rhizosphere of three different host plants within sampling sites of Woopo Wetlands.

Table 1. Relative abundance and community sturctures of arbuscular mycorrhizal fungal spores isolated from rhizospheres of different host plants in Upo Wetlands.

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Table 2. Relative abundance and community sturctures of arbuscular mycorrhizal fungal spores isolated from different sampling sites of Upo Wetlands.

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