한국작물학회지 (KOREAN JOURNAL OF CROP SCIENCE)

  • 제64권1호
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  • Pages.25-32
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  • 2019
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  • 0252-9777(pISSN)
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  • 2287-8432(eISSN)
한국작물학회 (The Korean Society of Crop Science)
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가을재배 찰옥수수의 등숙에 따른 지방산 조성 및 Phytosterol 함량 변화

Changes in Fatty Acid Composition and Phytosterol Content During Ripening Period of the Autumn-Sown Waxy Corn

  • 김선림 (농촌진흥청 국립식량과학원 중부작물부 중부작물과) ;
  • 김미정 (농촌진흥청 연구정책국 연구운영과) ;
  • 정건호 (농촌진흥청 국립식량과학원 중부작물부 재배환경과) ;
  • 이진석 (농촌진흥청 국립식량과학원 중부작물부 중부작물과) ;
  • 손범영 (농촌진흥청 국립식량과학원 중부작물부 중부작물과) ;
  • 김정태 (농촌진흥청 국립식량과학원 중부작물부 중부작물과) ;
  • 배환희 (농촌진흥청 국립식량과학원 중부작물부 중부작물과) ;
  • 고영삼 (농촌진흥청 국립식량과학원 중부작물부 중부작물과) ;
  • 백성범 (농촌진흥청 국립식량과학원 중부작물부 중부작물과)
  • Kim, Sun-Lim (Central Area Crop Breeding Division, Department of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Mi-Jung (Research Policy Bureau of Rural Development Administration) ;
  • Jung, Gun-Ho (Crop Cultivation and Environment Research Division, Department of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration) ;
  • Lee, Jin-Seok (Central Area Crop Breeding Division, Department of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration) ;
  • Son, Beom-Young (Central Area Crop Breeding Division, Department of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Jung-Tae (Central Area Crop Breeding Division, Department of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration) ;
  • Bae, Hwan-Hee (Central Area Crop Breeding Division, Department of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration) ;
  • Go, Young-Sam (Central Area Crop Breeding Division, Department of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration) ;
  • Baek, Seong-Bum (Central Area Crop Breeding Division, Department of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration)
  • 투고 : 2019.01.23
  • 심사 : 2019.03.14
  • 발행 : 2019.03.31
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초록

찰옥수수 가을재배시 등숙에 따른 종실의 지방산조성과 phytosterol의 함량변화를 검토하여 고품질 풋옥수수 생산을 위한 기초자료로 활용하고자 본 연구를 수행하여 얻어진 결과를 요약하면 다음과 같다. 1. 찰옥수수 출사후 일수가 경과할수록 종실의 조지방 함량은 지속적으로 증가 하였고, 흑진주찰은 일미찰보다 조지방 함량이 높았다. 2. 출사후 일수에 따른 지방산의 조성비는 뚜렷한 변화를 보였는데, palmitic acid 및 linoleic acid는 점차 감소되었으나 oleic acid는 증가되었고, 흑진주찰은 일미찰에 비해 불포화지방산의 조성비가 다소 높았다. 3. 출사후 일수가 경과할수록 총 phytosterol의 함량은 증가하여 출사후 33일에 함량의 최대치를 보였는데, 일미찰의 총 phytosterol 함량은 937.7 mg/100 g, 흑진주찰은 867.9 mg/100 g이었으나 품종간 통계적 함량의 차이는 없었다. 4. Phytosterol의 조성은 품종간 차이를 보였는데, 일미찰은 ${\beta}-sitosterol$ > stigmasterol > campesterol 순으로 함량이 높았으나, 흑진주찰은 ${\beta}-sitosterol$ > campesterol > stigmasterol 순으로 함량이 높았고, 성분별로 볼 때 campesterol 함량은 품종간 차이가 없었으나 stigmasterol 및 ${\beta}-sitosterol$은 흑진주찰이 일미찰에 비해 함량이 높았다. 5. Palmitic, linolieic, linolenic acid는 campesterol 및 ${\beta}-sitosterol$과 유의한 부(-)상관이 있었고, stearic acid는 stigmasterol과 정(+)상관이 인정되었다. Oleic acid는 campesterol 및 ${\beta}-sitosterol$과 정상관이 있었으나 stigmasterol과는 부상관이 인정되어 찰옥수수 등숙중 종실의 지방산 조성은 phytosterol의 함량의 변이에 영향을 미치는 것으로 판단되었다.

This study was carried out to investigate the changes in fatty acid composition and phytosterol content of the autumn sowing waxy corn during its ripening period for the production of high quality and value-added fresh ears. The content of crude oil in the waxy corn kernels steadily increased with days after pollination(DAP); the crude oil content in Heukjinjuchal (HC) was higher than that in Ilmichal (IC). The composition of fatty acids according to DAP showed a statistically significant change; palmitic and linoleic acid composition gradually decreased, but oleic acid increased, and the composition of unsaturated fatty acids in HC was higher than that in IC. The phytosterol content was increased until 33 DAP, and was 937.7 mg/100g and 867.9 mg/100g in IC and HC, respectively, but there was no statistically significant difference between the varieties. The phytosterol contents in IC varied in the following order: ${\beta}-sitosterol$ > stigmasterol > campesterol, but that in HC varied in the order ${\beta}-sitosterol$ > campesterol > stigmasterol. The campesterol content did not show significant differences between the varieties, but the stigmasterol and ${\beta}-sitosterol$ content was significantly higher in HC than in IC. Palmitic, linolieic, and linolenic acid were significantly correlated with campesterol and ${\beta}-sitosterol$, and stearic acid was positively correlated with stigmasterol. Oleic acid was positively correlated with campesterol and ${\beta}-sitosterol$ but negatively correlated with stigmasterol. As a result, it was suggested that fatty acids may affect the variation in phytosterol contents during the ripening period in waxy corn.

키워드

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Fig. 1. Changes in two waxy corn ears, Ilmichal and Heukjinjuchal, during the ripening period.

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Fig. 2. Schematic representation of the BSA[N,O-bis(trimethylsilyl) acetamide] silylation process using unsaponifiables in waxy corn kernels.

JMHHBK_2019_v64n1_25_f0003.png 이미지

Fig. 3. Changes in 100-kernel weights and crude oil contents in two waxy corn varieties, Ilmichal and Heukjinjuchal, during the ripening period.

JMHHBK_2019_v64n1_25_f0004.png 이미지

Fig. 4. Comparison of phytosterol chromatogram profiles in two waxy corn varieties, Ilmichal and Heukjinjuchal, at 30 days after pollination.

Table 1. Comparison of fatty acid compositions in two waxy corn varieties, Ilmichal and Heukjinjuchal, during the ripening period.

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Table 2. Comparison of content of phytosterols in two waxy corn varieties, Ilmichal and Heukjinjuchal, during the ripening period.

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Table 3. Relationship among crude oil, phytosterols, and fatty acids during the ripening period of waxy corns.

JMHHBK_2019_v64n1_25_t0003.png 이미지

Table 4. Relationship between fatty acids and phytosterols during the ripening of waxy corns.

JMHHBK_2019_v64n1_25_t0004.png 이미지

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