Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Immune Response of BALB/c Mice toward Putative Calcium Transporter Recombinant Protein of Trichomonas vaginalis

  • Mendoza-Oliveros, Tahali (Laboratorio de Bioquimica y Genetica Molecular, Facultad de Quimica de la Universidad Autonoma de Yucatan) ;
  • Arana-Argaez, Victor (Laboratorio de Farmacologia, Facultad de Quimica de la Universidad Autonoma de Yucatan) ;
  • Alvarez-Sanchez, Leidi C. (Laboratorio de Virologia, Centro de Investigaciones Regionales "Dr. Hideyo Noguchi" de la Universidad Autonoma de Yucatan) ;
  • Lara-Riegos, Julio (Laboratorio de Bioquimica y Genetica Molecular, Facultad de Quimica de la Universidad Autonoma de Yucatan) ;
  • Alvarez-Sanchez, Maria Elizbeth (Posgrado en Ciencias Genomicas, Universidad Autonoma de la Ciudad de Mexico (UACM)) ;
  • Torres-Romero, Julio C. (Laboratorio de Bioquimica y Genetica Molecular, Facultad de Quimica de la Universidad Autonoma de Yucatan)
  • Received : 2018.08.11
  • Accepted : 2018.12.23
  • Published : 2019.02.28
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Abstract

Trichomoniasis is a common sexually transmitted infection caused by Trichomonas vaginalis, which actually does not exist a vaccine for control or prevention. Thus, the identification of new and potent immunogens in T. vaginalis, which can contribute to the development of a vaccine against this parasite, is necessary. Therefore, the aim of this work was to evaluate the potential of a recombinant Transient Receptor Potential-like channel of T. vaginalis (TvTRPV), as a promising immunogen in BALB/c mice. First, TvTRPV was cloned and expressed as a recombinant protein in Escherichia coli BL21 cells and purified by nickel affinity. Next, BALB/c mice were immunized and the antibody levels in mice serum and cytokines from the supernatant of macrophages and from co-culture systems were evaluated. Recombinant TvTRPV triggered high levels of specific total IgG in sera from the immunized mice. Also, a statistically significant increase of cytokines: $IL-1{\beta}$, IL-6, and $TNF-{\alpha}$ after stimulation with the corresponding antigens in vitro, was identified. Moreover, co-cultures using $CD4^+$ T cells from immunized mice were able to identify higher levels of IL-10 and $IFN-{\gamma}$. These results were useful to validate the immunogenicity of TvTRPV in BALB/c mice, where IL-10-$IFN-{\gamma}$-secreting cells could play a role in infection control, supporting the potential of TvTRPV as a promising target for vaccine against T. vaginalis.

Keywords

References

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