Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Comparison of Two PCR Assays for Trichomonas vaginalis

  • Noh, Chang-Suk (Department of Internal Medicine, Hallym University Hangang Sacred Heart Hospital) ;
  • Kim, Sang-Su (Department of Environmental Biology and Medical Parasitology, Hanyang University Graduate School of Biomedical Science and Engineering) ;
  • Park, Sung-Yul (Department of Urology, Hanyang University College of Medicine) ;
  • Moon, Hong-Sang (Department of Urology, Hanyang University College of Medicine) ;
  • Hong, Yeonchul (Department of Parasitology and Tropical Medicine, Kyungpook National University School of Medicine) ;
  • Ryu, Jae-Sook (Department of Environmental Biology and Medical Parasitology, Hanyang University Graduate School of Biomedical Science and Engineering)
  • Received : 2018.11.19
  • Accepted : 2019.01.31
  • Published : 2019.02.28
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Abstract

PCR is known to be the most sensitive method for diagnosing Trichomonas vaginalis infections. This study aimed to compare the sensitivity of a PCR assay for trichomoniasis (HY-PCR) developed in Hanyang University with the use of a Seeplex Ace Detection $Kit^{(R)}$, using urine collected from four Korean men with prostatic disease. Overall, HY-PCR was more sensitive than the Seeplex Kit. The use of Chelex 100 is recommended for DNA isolation in order to increase the sensitivity of the PCR test.

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References

  1. Bouchemal K, Bories C, Loiseau PM. Strategies for prevention and treatment of Trichomonas vaginalis infections. Clin Microbiol Rev 2017; 30: 811-825. https://doi.org/10.1128/CMR.00109-16
  2. Fichorova RN. Impact of T. vaginalis infection on innate immune responses and reproductive outcome. J Reprod Immunol 2009; 83: 185-189. https://doi.org/10.1016/j.jri.2009.08.007
  3. Moodley D, Sartorius B, Madurai S, Chetty V, Maman S. Pregnancy outcomes in association with STDs including genital HSV-2 shedding in a South African cohort study. Sex Transm Infect 2017; 93: 460-466. https://doi.org/10.1136/sextrans-2017-053113
  4. Mercer F, Johnson PJ. Trichomonas vaginalis: Pathogenesis, symbiont interactions, and host cell immune responses. Trends Parasitol 2018; 34: 683-693. https://doi.org/10.1016/j.pt.2018.05.006
  5. Mukanyangezi MF, Sengpiel V, Manzi O, Tobin G, Rulisa S, Bienvenu E, Giglio D. Screening for human papillomavirus, cervical cytological abnormalities and associated risk factors in HIV-positive and HIV-negative women in Rwanda. HIV Med 2018; 19: 152-166. https://doi.org/10.1111/hiv.12564
  6. Rendon-Maldonado J, Espinosa-Cantellano M, Soler C, Torres JV, Martinez-Palomo A. Trichomonas vaginalis: in vitro attachment and internalization of HIV-1 and HIV-1-infected lymphocytes. J Eukaryot Microbiol 2003; 50: 43-48. https://doi.org/10.1111/j.1550-7408.2003.tb00104.x
  7. Guenthner PC, Secor WE, Dezzutti CS. Trichomonas vaginalis-induced epithelial monolayer disruption and human immunodeficiency virus type 1 (HIV-1) replication: implications for the sexual transmission of HIV-1. Infect Immun 2005; 73: 4155-4160. https://doi.org/10.1128/IAI.73.7.4155-4160.2005
  8. Garber GE, Sibau L, Ma R, Proctor EM, Shaw CE, Bowie WR. Cell culture compared with broth for detection of Trichomonas vaginalis. J Clin Microbiol 1987; 25: 1275-1279. https://doi.org/10.1128/JCM.25.7.1275-1279.1987
  9. Kaydos-Daniels SC, Miller WC, Hoffman I, Banda T, Dzinyemba W, Martinson F, Cohen MS, Hobbs MM. Validation of a urinebased PCR-enzyme-linked immunosorbent assay for use in clinical research settings to detect Trichomonas vaginalis in men. J Clin Microbiol 2003; 41: 318-323. https://doi.org/10.1128/JCM.41.1.318-323.2003
  10. Lee JJ, Moon HS, Lee TY, Hwang HS, Ahn MH, Ryu JS. PCR for diagnosis of male Trichomonas vaginalis infection with chronic prostatitis and urethritis. Korean J Parasitol 2012; 50: 157-159. https://doi.org/10.3347/kjp.2012.50.2.157
  11. Dierkes C, Ehrenstein B, Siebig S, Linde HJ, Reischl U, Salzberger B. Clinical impact of a commercially available multiplex PCR system for rapid detection of pathogens in patients with presumed sepsis. BMC Infect Dis 2009; 9: 126. https://doi.org/10.1186/1471-2334-9-126
  12. Lee SJ, Park DC, Lee DS, Choe HS, Cho YH. Evaluation of $Seeplex^{(R)}$ STD6 ACE Detection kit for the diagnosis of six bacterial sexually transmitted infections. J Infect Chemother 2012; 18: 494-500. https://doi.org/10.1007/s10156-011-0362-7
  13. Madico G, Quinn TC, Rompalo A, McKee KT Jr, Gaydos CA. Diagnosis of Trichomonas vaginalis infection by PCR using vaginal swab samples. J Clin Microbiol 1998; 36: 3205-3210. https://doi.org/10.1128/JCM.36.11.3205-3210.1998
  14. Paces J, Urbankova V, Urbanek P. Cloning and characterization of a repetitive DNA sequence specific for Trichomonas vaginalis. Mol Biochem Parasitol 1992; 54: 247-255. https://doi.org/10.1016/0166-6851(92)90116-2
  15. Seo JH, Yang HW, Joo SY, Song SM, Lee YR, Ryu JS, Yoo ES, Lee WK, Kong HH, Lee SE, Lee WJ, Goo YK, Chung DI, Hong Y. Prevalence of Trichomonas vaginalis by PCR in men attending a primary care urology clinic in South Korea. Korean J Parasitol 2014; 52: 551-555. https://doi.org/10.3347/kjp.2014.52.5.551
  16. Strom GE, Tellevik MG, Hanevik K, Langeland N, Blomberg B. Comparison of four methods for extracting DNA from dried blood on filter paper for PCR targeting the mitochondrial Plasmodium genome. Trans R Soc Trop Med Hyg 2014; 108: 488-494. https://doi.org/10.1093/trstmh/tru084

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