한국식품과학회지 (Korean Journal of Food Science and Technology)

한국식품과학회 (Korean Society of Food Science and Technology)
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고지방식이 마우스에서 3,5-dicaffeoylquinic acid의 항비만 효과

Anti-obesity effect of 3,5-dicaffeoylquinic acid on high-fat diet mouse

  • 강진용 (경상대학교 응용생명과학부(BK12 plus), 농업생명과학연구원) ;
  • 박선경 (경상대학교 응용생명과학부(BK12 plus), 농업생명과학연구원) ;
  • 김종민 (경상대학교 응용생명과학부(BK12 plus), 농업생명과학연구원) ;
  • 박수빈 (경상대학교 응용생명과학부(BK12 plus), 농업생명과학연구원) ;
  • 유슬기 (경상대학교 응용생명과학부(BK12 plus), 농업생명과학연구원) ;
  • 한혜주 (경상대학교 응용생명과학부(BK12 plus), 농업생명과학연구원) ;
  • 김대옥 (경희대학교 식품생명공학과) ;
  • 허호진 (경상대학교 응용생명과학부(BK12 plus), 농업생명과학연구원)
  • Kang, Jin Yong (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Park, Seon Kyeong (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kim, Jong Min (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Park, Su Bin (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Yoo, Seul Ki (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Han, Hye Ju (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kim, Dae Ok (Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Heo, Ho Jin (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University)
  • 투고 : 2018.11.16
  • 심사 : 2018.12.30
  • 발행 : 2019.02.28
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초록

이연구는 고지방식이로 인한 비만 및 대사장애에 대한 3,5-diCQA의 효과를 확인하는 동시에 클로로겐산과의 상대적인 생리 활성을 확인하였다. 클로로겐산은 이전에 보고된 연구결과에서처럼 고지방식이를 섭취하는 쥐의 체중 증가를 효과적으로 억제하였으며, 3,5-diCQA와 비교하였을 때에도 그 효과가 상대적으로 우수하였다. 내장지방의 무게를 측정한 결과 3,5-diCQA와 클로로겐산 모두 내장지방의 축적을 효과적으로 억제하였으며, 이러한 이유는 두 샘플 모두 지방조직에서의 AMPK의 활성화가 증가된 것과 관련이 있는 것으로 보인다. 또한 이 두 샘플은 비만으로 인해 발생하는 산화적 스트레스로부터 간조직을 보호하는 효과가 있었다. 그러나 간조직에서의 지방축적을 확인한 결과에서 클로로겐산은 여전히 간의 지방축적을 억제하는 것으로 확인되었지만 3,5-diCQA는 오히려 간의 지방축적을 증가시킨 것이 확인되었다. 이러한 결과는 3,5-diCQA가 간조직에서 Akt의 활성을 증가시킨 것과 관련이 있는 것으로 보이며 이 증가된 Akt로 인해 3,5-diCQA 그룹의 내당능 개선이 클로로겐산 그룹보다도 우수하였지만 간에서의 지방 축적을 증가시킨 것으로 판단된다.

This study was performed to confirm the influence of chlorogenic acid (CGA) and 3,5-dicaffeyolquinic acid (3,5-diCQA) intake on problems caused by high-fat diet. CGA was more effective in suppressing weight gain than 3,5-diCQA. In contrast, 3,5-diCQA was more effective in improving glucose tolerance than CGA. In the biopsy, it was confirmed that CGA inhibited visceral fat and liver fat accumulation. 3,5-diCQA also inhibited visceral fat accumulation, but 3,5-diCQA increased liver fat accumulation. The liver fat accumulation induced oxidative stress, but 3,5-diCQA reduced oxidative damage through its antioxidant activity. The increased liver fat accumulation was because a 3,5-diCQA greatly increased Akt phosphorylation and decreased AMPK phosphorylation in the liver. Consequently, CGA was effective in alleviating the problems caused by high-fat diets, while maintaining normal balance. 3,5-diCQA also showed a positive effect on problems caused by high-fat diets, but it increased liver fat accumulation and thereby had negative consequences.

키워드

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Fig. 1 The effect of CGA and 3,5-diCQA intake in HFD-induced obese mice on body weight change.

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Fig. 2 The effect of CGA and 3,5-diCQA intake in HFD-induced obese mice on glucose tolerance.

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Fig. 3 The effect of CGA and 3,5-diCQA intake in HFD-induced obese mice on lipid accumulation.

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Fig. 4 The effect of CGA and 3,5-diCQA intake in HFD-induced obese mice on liver damage.

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Fig. 5 The effect of CGA and 3,5-diCQA intake HFD-induced obese mice on protein expression of p-AMPK/β-actin in epididymal adipose (A) and liver tissue (B) and p-Akt/β-actin in epididymal adipose (C) and liver tissue (D) and representative image (E).

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Fig. 5. Continued

Table 1. Effect of CGA and 3,5-diCQA on body weight changes and food intake in HFD-induced obese mice

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Table 2. Effect of CGA and 3,5-diCQA on serum biomarker in HFD-induced obese mice

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Table 3. Effect of CGA and 3,5-diCQA on serum lipid biomarker in HFD-induced obese mice

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