Fig. 2. Fluorescence distribution observed in colonies transformed with eGFP, mRFP1 and CFP-SKL. Microscope slides were mounted with mycelia from a 5-day-old culture grown on 20%-V8 plates. The scale bar represents 25 μm.
Fig. 3. GFP-labeled P. palmivora during colonization at different times in oil palm immature leaflets. A: Appressorium, GC: Germinating cyst, AH: Apoplastic hyphae, HS: Hyphal swelling, H: Haustorium, Sp: Sporangium. The scale bar represents 50 μm.
Fig. 4. Necrotic lessons observed on immature leaflets inoculated with a WT and GFP-tagged P. palmivora. (A) Soft tissue from minus five leaf and rigid immature tissue from minus three leaf inoculated with P. palmivora, observed at four days post-inoculation. (B) Wounded or unwounded green spear leaflets four days after inoculation with P. palmivora. The red arrow shows the site that were wounded with a sterile syringe needle.
Fig. 5. Cell death responses observed in distal and rigid parts of immature leaflets inoculated with a GFP-tagged transformant and counterstained with PI. (A) Proximal soft tissue inoculated at 24 hpi. (B) Germinating cysts of P. palmivora inoculated in a distal part of an immature leaflet at two hpi. (C), (D) Oil palm cell death responses in a rigid immature leaflet at 12 (C) and 48 (D) hpi, respectively. The scale bars represent 100 μm. A: Appressorium, GC: Germinating cyst, PpN: P. palmivora cell nucleus, EgN: Oil palm (E. guineensis) cell nucleus.
Fig. 1. (A) Schematic representation of the T-DNA included in the binary plasmid pNPTII. FP: Fluorescent protein. (B) Representative evaluation of transformant colonies by Southern blot with nptII probe three months after transformation; a wild type (WT) isolate was used as a negative control. Dashed lines show the colonies selected for monosporic cultures. The bottom blot shows the hybridization obtained from colonies derived from different single zoospore cultures.
Table 1. List of primers used in this study
Table 2. Number of transformant colonies obtained, discriminated by isolate, fluorescent protein and copy number determined by South-ern blot
Table 3. Comparisons of colony diameter and lesson length in oil palm leaflets of different transformants
참고문헌
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