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http://dx.doi.org/10.5423/PPJ.OA.02.2018.0034

Visualization of Phytophthora palmivora Infection in Oil Palm Leaflets with Fluorescent Proteins and Cell Viability Markers

Ochoa, Juan C. (Oil Palm Biology and Breeding Research Program, Colombian Oil Palm Research Center (CENIPALMA))
Herrera, Mariana (Oil Palm Biology and Breeding Research Program, Colombian Oil Palm Research Center (CENIPALMA))
Navia, Monica (Oil Palm Biology and Breeding Research Program, Colombian Oil Palm Research Center (CENIPALMA))
Romero, Hernan Mauricio (Oil Palm Biology and Breeding Research Program, Colombian Oil Palm Research Center (CENIPALMA))

Publication Information

The Plant Pathology Journal / v.35, no.1, 2019 , pp. 19-31 More about this Journal

Abstract

Bud rot (BR) is the most devastating disease affecting oil palm (Elaeis guineensis) crops in Colombia. Its causal agent, Phytophthora palmivora, initiates the infection in immature oil palm leaflets producing necrotic lesions, followed by colonization of opportunistic necrotrophs, which increases disease damage. To improve the characterization of the disease, we transformed P. palmivora using Agrobacterium tumefaciens-mediated transformation (ATMT) to include the fluorescent proteins CFP-SKL (peroxisomal localization), eGFP and mRFP1 (cytoplasmic localization). The stability of some transformants was confirmed by Southern blot analysis and single zoospore cultures; additionally, virulence and in vitro growth were compared to the wild-type isolate to select transformants with the greatest resemblance to the WT isolate. GFP-tagged P. palmivora was useful to identify all of the infective structures that are commonly formed by hemibiotrophic oomycetes, including apoplastic colonization and haustorium formation. Finally, we detected cell death responses associated with immature oil palm tissues that showed reduced susceptibility to P. palmivora infection, indicating that these tissues could exhibit age-related resistance. The aim of this research is to improve the characterization of the initial disease stages and generate cell biology tools that may be useful for developing methodologies for early identification of oil palm materials resistant or susceptible to BR.

Keywords

age-related resistance; A. tumefaciens-mediated transformation; cell death responses; GFP; propidium iodide;

Citations & Related Records

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