DOI QR코드

DOI QR Code

한국산 왜몰개 Aphyocypris chinensis 정자의 미세해부학적 구조 (Pisces: Cyprinidae)

Fine Structure of Spermatozoa of Venus Fish, Aphyocypris chinensis (Pisces: Cyprinidae) from Korea

  • Kim, Kgu-Hwan (Department of Radiologic Technology, Daegu Health Colleage) ;
  • Ahn, Sung-Ju (Department of Clinical Pathology, Daegu Health Colleage)
  • 투고 : 2019.10.16
  • 심사 : 2019.12.11
  • 발행 : 2019.12.31

초록

Aphocypris chinensis 정자의 구조는 잉어류 정자의 일반적인 구조와 비슷하나 핵의 구조, 중심립의 위치, 미토콘드리아의 수와 배열 그리고 편모의 구조에서 차이가 있었다. A. chinensis 정자의 핵와가 기부중심립을 포함하고 있는 것은 잉어류 정자의 공통된 특징으로 볼 수 있다. 섬유성물질에 의하여 원형질막과 연결된 중심립은 말단부중심립의 기저부 위치까지 cytoplasmic canal이 함입되어 있다. 이와 같은 구조는 잉어류의 공통된 특징으로 사료된다. 잉어류 정자의 미토콘드리아의 수를 구분해보면, 4개의 그룹(1개, 2~5개, 6~10개, 11개 이상)으로 구분할 수 있다. A. chinensis는 잉어류 정자에서 지금까지 보고된 미토콘드리아의 수가 가장 많은 종이다. 잉어류 정자에서 나타나는 중편부와 미부에 분포하는 vesicles의 분포형태도 어류 정자의 미세해부학적 분류의 형질로 활용할 수 있을 것으로 사료된다. 편모의 위치가 핵의 측면으로 위치해 있는 것은 A. chinensis를 포함한 대부분 잉어류 정자의 공통된 특징으로 사료된다.

Spermatozoa of Aphyocypris chinensis was studied by transmission electron microscopy. The investiation revaled that, spermatozoa display a round head, a small midpiece and a long tail region. In the spermatozoa, the nucleus contains highly condensed homogeneous chromation with small electron lucent areas. The base of the nucleus is slightly invaginated laterally by the nuclear fossa which contains the proximal centriole. The two centrioles orientated at an obtus angle (130°) to each other. The midpiece encircles the flagellum and is completely separated from it by the cytoplasmic channel. The midpiece contains more than 12 mitochondria. The mitochondria are arranged in 4~5 layer and are asymmetrically distributed in the postnuclear cytoplasm. The mitochondria surround the proximal part of the flagellum. The flagellum has classical 9+2 axoneme and no lateral fins. The spermatozoa of A. chinensis are similar to those of other cyprinids having a spherical head with a shallow nuclear fossa, a short midpiece including the asymmetrical arrangement of mitochondria and the lateral insertion of flagellum. However, there are some differences in the orientation of centrioles and the number of the mitochondria.

키워드

참고문헌

  1. Baccetti, B., A.G. Burrini, G. Callaini, G. Gibertini, M. Mazzini and S. Zerunian. 1984. Fish germinal cells I. Comparative spermatology of seven cyprinid species. Gamete Res., 10: 373-396. https://doi.org/10.1002/mrd.1120100405
  2. Guan, T.L. and B.A. Afzelius. 1991. The spermatozoon of the Chineses bitterling, Rhodeus sericeus sinensis (Cyprinidae, Teleostei). J. Submicrosc. Cytol. Pathol., 23: 351-356.
  3. Gunther, A. 1868. Catalog of the fishes in the British Musem. Catalogue of the physostomi, containing the families Heteropygii, Cyprinidae, Gonorhynchidae, Hyodontidae, Osteoglossidae, Clupeidae, Chirocentridae, Alepocephalidae, Notopteridae, Halosauridae, in B. Mus., v 7: i-xx+1-512.
  4. Jamieson, B.G.M. 1991. Fish evolution and systematics: Evidence from spermatozoa. Cambridge University Press, New York, pp. 135-148.
  5. Kenzo, U. 1993. Motility and morphology of sperm of the ayu, Plecoglass altivelis at different salinities. Japan, J. Ichthyol., 40: 273-278.
  6. Kim, I.S. 1997. Illustrated encyclopedia of fauna & flora of Korea, Vo. 37, Freshwater fishes. Ministry of Education, Yeongi, 267pp. (in Korean)
  7. Kim, I.S., G.Y. Lee and S.Y. Yang. 1985. Systematic study of the subfamily Leuciscinae (Cyprinidae) from Korea. Bull. Korean Fish. Soc., 18: 381-400. (in Korean)
  8. Kim, J.K., K.H. Kim and K.J. Hwang. 2008. Anatomical ultrastructure of spermiogenesis and spermatozoa of Microphysogobio yaluensis (Pisces: Cyprinidae) from Korea. Korean J. Ichthyol., 20: 7-12. (in Korean)
  9. Kim, K.H. 2006. Ultrastructure of Zacco koreanus (Teleostei, Cypriniforems, Cyprinidae) spermiogenesis and spermatozoa. Korean J. Ichthyol., 18: 347-354.
  10. Kim, K.H. 2008. Anatomical ultrastructure of spermatozoa of a Korean bitterling, Acheilognathus yamatsutae (Pisces, Cyprinidae). Korean J. Ichthyol., 20: 163-166. (in Korean)
  11. Kim, K.H. 2019. Anatomical ultrastructure of spermatozoa of Korean sharpbelly, Hemiculter eigenmanni (Cypriniformes, Cyprinidae). Korean J. Ichthyol., 31: 1-6. https://doi.org/10.35399/ISK.31.1.1
  12. Kim, K.H., A.S. Kwon and Y.H. Lee. 1998. Spermatozoal ultrastructure and phylogenetic relationships of the subfamily Gobioninae (Cyprinidae, Teleostei) Ultrastructure of the spermatozoa of the Korean gudgeon Squalidus chankaensis tsuchigae. Korean J. Limnol., 31: 159-164.
  13. Kim, K.H., D.M. Kwon, J.I. Lee and S.B. Jung. 2007a. Ultrastructure of Psedogobio esocinus (Teleostei, Cyprinidae) spermiogenesis and spermatozoa. J. Kor. Fish. Soc., 40: 147-152.
  14. Kim, K.H., J.K. Kim and K.J. Hwang. 2007b. Ultrastructure of spermatozoa of a Korean bitterling, Acheilognathus koreensis (Pisces, Cyprinidae). Korean J. Ichthyol., 19: 286-291.
  15. Kim, K.H. and Y.H. Lee. 2000. The ultrastructure of spermatozoa of the Ussurian bullhead, Leiocassis ussuriensis (Teleostei, Siluriformes, Bagridae) with phylogenetic consideration. Korean J. Limnol., 33: 405-412. (in Korean)
  16. Kim, Y.J., C.Y. Choi and I.S. Park. 2006. Ultrastructure of dark chub Zacco temminckii (Cyprinidae) spermatozoa. J. Fish. Sci. Technol., 9: 1-6.
  17. Lee, C.L. and I.S. Kim. 1987. An osteological study of the venus fish, Aphyocypris chinensis Gunther (Cyprinidae) from Korea. Korean J. Syst. Zool., 3: 41-50. (in Korean)
  18. Lee, Y.H. and K.H. Kim. 1998a. Spermatozoal ultrastructure and phylogenetic relationships of the subfamily Gobioninae (Cyprinidae, Teleostei) 1. Ultrastructure of the spermatozoa of the Korean Gudgeon Squalidus gracilis majimae. Korean J. Electron Microsc., 28: 63-71.
  19. Lee, Y.H. and K.H. Kim. 1998b. Ultrastructure of spermatozoa in Pungtungia herzi. Dev. Reprod., 2: 141-148.
  20. Lee, Y.H. and K.H. Kim. 2001. The ultrastructure of spermatozoa of the slender catfish Silurus microdorsalis (Teleostei, Siluriformes, Siluridae) with phylogenetic considerations. J. Submicrosc. Cytol. Pathol., 33: 329-336.
  21. Mattei, C. 1970. Spermiogeneses comparee des Poissons. In: Baccetti, B. (ed.), Comparative Spermatology. Academic Press, New York, pp. 57-69.
  22. Neznanova, S.U. 2012. Ultrastructural study of spermiogenesis in far-eastern redfins of the Genus Tribolodon (Pisces: Cyprinidae). Russian J. Marine Biol., 38: 156-165. https://doi.org/10.1134/S1063074012020071
  23. Park, J.Y. and I.S. Kim. 1996. Fine structure spermatozoa of Cobitidae (Pisces: Cypriniformes). Korean J. Ichtyol., 8: 74-83. (in Korean)