Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
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Antioxidant activity and polyphenol content of fermented Sparassis latifolia extracts

꽃송이버섯 발효물의 항산화 활성 및 폴리페놀 함량 변화

  • Yang, Seung-Hwa (Department of Chemical Engineering, Graduate School of Chosun University) ;
  • Lee, Yong-Jo (Industrial-Academic Cooperation Group, Kwangju Women's University) ;
  • Kim, Da-Song (Department of Chemical Engineering, Graduate School of Chosun University) ;
  • Shin, Hyun-Jae (Department of Chemical Engineering, Graduate School of Chosun University)
  • 양승화 (조선대학교 대학원 화학공학과) ;
  • 이용조 (광주여자대학교 산학협력단) ;
  • 김다송 (조선대학교 대학원 화학공학과) ;
  • 신현재 (조선대학교 대학원 화학공학과)
  • Received : 2019.12.17
  • Accepted : 2019.12.20
  • Published : 2019.12.31
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Abstract

Sparassis latifolia is a useful medicinal mushroom that has recently gained popularity in Asia. It has a rich flavor and is a good source of nutrients contains a large number of polyphenols for a functional food or dietary supplement. In addition, S. latifolia is rich in beta-glucan and gamma-aminobutyric acid (GABA). These two compounds have been reported to show immune-stimulating and anticancer effects by numerous studies. In this study, four species of lactic acid bacteria (Lactobacillus plantarum subsp. plantarum, L. acidophilus, L. helveticus, and L. delbrueckii subsp. bulgaricus) were used to ferment the fruiting body of S. latifolia. Fermented S. latifolia extracts were found to have a higher polyphenol content and antioxidant activity following fermentation as well as increased protease activity.

본 논문에서는 꽃송이버섯을 4종의 유산균(Lactobacillus plantarum subsp. plantarum, L. acidophilus, L. helveticus, L. delbrueckii subsp. bulgaricus)을 사용해 발효를 하였다. 발효된 꽃송이버섯 발효물을 항산화 활성(DPPH free radical scavenging activity, ABTS radical scavenging activity, Hydroxy radical scavenging activity), SOD activity, 총 폴리페놀 함량(TPC), HPLC 분석, protease 활성을 동해 발효 전 후 생리활성 변화를 확인하였다. 꽃송이버섯 발효물의 항산화 활성은 IC50 28.09-64.46 ㎍/mL, SOD 활성은 IC50 15.75-39.42 ㎍/mL, TPC는 57.66-100.43 GAE ㎍/mL 와 85.25-110.68 ㎍/mL 로 확인되었다. 꽃송이버섯 추출물의 항산화 활성보다 약 8배 이상 증가된 결과로 확인되었다. 또한 꽃송이버섯 발효물의 HPLC 분석 결과는 ascorbic acid, gallic acid, chlorogenic acid의 함량이 높게 확인되었으며, protease 활성 또한 발효물의 활성이 높은 것으로 확인되었다. Lactobacillus 균주들의 작용에 의하여 꽃송이버섯이 발효되면서 폴리페놀류가 증가하여 생리활성이 꽃송이버섯 추출물에 비해 증가한 것으로 추측된다. Lactobacillus 균중에서 L. helveticus 균주로 발효된 꽃송이버섯 발효물은 가장 높은 활성을 나타냈으며, 꽃송이버섯을 발효하는데 있어 L. helveticus 균주가 가장 적합한 균주로 확인되었다.

Keywords

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