한국버섯학회지 (Journal of Mushroom)

  • 제17권4호
  • /
  • Pages.261-267
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  • 2019
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  • 1738-0294(pISSN)
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  • 2288-8853(eISSN)
한국버섯학회 (The Korean Society of Mushroom Science)
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먹이원에 따른 흰점박이꽃무지(Protaetia brevitarsis) 유충의 항산화활성

Variations in antioxidant activity in Protaetia brevitarsis larvae depending on the feeding source

  • 김혜수 (경남과학기술대학교 제약공학과) ;
  • 박현영 (경남과학기술대학교 제약공학과) ;
  • 권현숙 (한국한의약진흥원) ;
  • 이상호 (농업회사법인 도다움) ;
  • 하준 (농업회사법인 도다움) ;
  • 이상원 (경남과학기술대학교 제약공학과) ;
  • 조수정 (경남과학기술대학교 제약공학과)
  • Kim, Hye Soo (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology) ;
  • Park, Hyun-Young (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology) ;
  • Kwon, Hyun-Sook (National Institute of Korean Medicine Development) ;
  • Lee, Sang-Ho (Agricultural Corporation Company Dodaum) ;
  • Ha, Jun (Agricultural Corporation Company Dodaum) ;
  • Lee, Sang-Won (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology) ;
  • Cho, Soo-Jeong (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology)
  • 투고 : 2019.12.06
  • 심사 : 2019.12.23
  • 발행 : 2019.12.31
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초록

본 연구에서는 기능성 식품원료로써 흰점박이꽃무지 유충의 이용 가능성과 흰점박이꽃무지 유충 사료자원으로써 큰느타리버섯수확후배지의 이용 가능성을 조사하기 위해서 참나무톱밥과 큰느타리수확후배지를 각각 식이한 흰점박이 꽃무지 유충 추출물의 항산화 활성을 비교하였다. 참나무톱밥과 큰느타리수확후배지를 각각 식이한 흰점박이꽃무지 유충 추출물의 총 폴리페놀 함량은 각각 57.02±1.73 mg GAE/g과 75.33±0.43 mg GAE/g이었고 플라보노이드 함량은 각각 24.6±0.28 mg/g과 25.4±0.75 mg/g이었다. DPPH에 의한 라디칼소거 활성은 추출물의 농도가 증가할수록 증가하는 경향을 나타내었으나 참나무톱밥을 식이한 흰점박이꽃무지 유충에 비해 큰느타리버섯수확후배지를 식이한 흰점박이꽃무지 유충 추출물의 DPPH 라디칼 소거능이 우수하였으며 32 mg/ml 이상의 농도에서는 두 시료 간의 유의적 차이가 나타나지 않았다. 참나무톱밥과 큰느타리버섯수확후배지를 각각 식이한 흰점박이꽃무지 유충 추출물의 농도별 환원력도 추출물의 농도가 높을수록 환원력은 증가하였으나 시료 간의 유의적인 차이는 보이지 않았다. 참나무톱밥과 큰느타리버섯수확후배지를 식이한 흰점박이꽃무지 유충 추출물의 ORAC 지수는 각각 66.55±0.99 uM TE/g과 76.32±0.48 uM TE/g로 참나무톱밥을 식이한 흰점박이꽃무지 유충 추출물에 비해 큰느타리버섯수확후배지를 식이한 흰점박이꽃무지 유충 추출물의 ORAC 지수가 높게 나타났다. 참나무톱밥과 큰느타리버섯수확후배지를 각각 식이한 흰점박이꽃무지 유충 추출물을 각각 0, 2, 4, 8, 16, 32, 64 mg/ml의 농도로 처리하였을 때 RAW 264.7 세포는 90% 이상의 생존율을 나타내었으므로 참나무톱밥과 큰느타리버섯수확후배지를 각각 식이한 흰점박이꽃무지 유충 추출물은 RAW 264.7 세포에 독성을 나타내지 않는 것으로 판단된다.

The objective of this study was to evaluate the antioxidant activity of extracts of Protaetia brevitarsis larvae fed on fermented oak sawdust (FOS) or spent mushroom substrates (SMS, Pleurotus eryngii). Total polyphenol content was 32% higher in extracts of larvae fed on SMS (P. eryngii) (75.33±0.43 mg GAE/g) than in extracts of larvae fed on FOS (57.02±1.73 mg GAE/g). The flavonoid content of extracts of larvae grown on FOS and SMS (P. eryngii) was 24.6±0.28 mg/g and 25.4±0.75 mg/g, respectively. DPPH radical scavenging activity increased in an extract concentration-dependent manner, and the DPPH radical scavenging capacity of the extract of larvae produced on SMS (P. eryngii) was higher than that of the larvae produced on FOS. The reducing power of the larval extracts produced on FOS and SMS (P. eryngii) increased in an extract concentration-dependent manner, but there was no significant difference between them. The extract of larvae fed on SMS (P. eryngii) (66.55±0.99 uM TE/g) had a higher oxygen radical absorbance capacity (ORAC) than extracts of larvae grown on FOS (76.32±0.48 uM TE/g). The effect of larval extracts on cell proliferation was investigated using a WST-1 (4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate) assay on RAW 264.7 cells. When cells were treated with larval extracts produced on FOS and SMS (P. eryngii) at concentrations of 0, 2, 4, 8, 16, 32, 40, and 64 mg/ml, RAW 264.7 cells proliferated at 90% or more. Therefore, larval extracts produced on FOS and SMS (P. eryngii) were not toxic to RAW 264.7 cells.

키워드

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