Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Effect of Red Ginseng Extract on the Inflammatory Response of LPS-Induced RAW 264.7 cell

홍삼추출물이 LPS로 유도된 RAW 264.7 cell의 염증반응에 미치는 효과

  • Jang, Young-Ah (Daegu Haany University, Faculty of Bio Industry Department of Cosmeceutical) ;
  • Kim, Han-Na (Mokwon University, College of Science & Technology, Division of Biomedicinal & Cosmetics) ;
  • Kim, Bo-Ae (Mokwon University, College of Science & Technology, Division of Biomedicinal & Cosmetics)
  • 장영아 (대구한의대학교 바이오산업융합학부 화장품약리학과) ;
  • 김한나 (목원대학교 생의약화장품학부 화장품전공) ;
  • 김보애 (목원대학교 생의약화장품학부 화장품전공)
  • Received : 2019.12.04
  • Accepted : 2019.12.28
  • Published : 2019.12.30
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Abstract

We conducted this study to investigate anti-inflammatory possibilities of applying cosmetic material about extracts from red ginseng. For this we carried out biological active evaluation about anti-inflammatory by using extracts of red ginseng. In order to evaluate the anti-inflammatory effects of the samples in macrophages (RAW 264.7 cells), MTT assay was used to evaluate the toxicity of red ginseng extracts and the inhibitory activity of nitric oxide production and the expression levels of inflammation-related proteins and genes. The inhibitory activity of nitric oxide in the LPS-induced RAW 264.7 cells was 71.2% at 25 ㎍/ml concentration and western blot analysis showed that the expression of iNOS and COX-2 protein decreased in a concentration-dependent manner. These results suggest that extracts from red ginseng may have value as the potential cosmetic materials.

본 연구는 홍삼추출물의 화장품소재로서의 항염증 효과의 가능성을 확인하는 것을 목적으로 한다. 본 연구에서는 홍삼 추출물을 사용하여 항염증에 대한 생물학적 활성평가를 수행하였다. 대식세포인 RAW 264.7 세포에서 시료의 항염증 효과를 평가하기 위해 MTT assay를 이용한 홍삼 추출물의 독성평가와 nitric oxide 생성 저해 활성 및 염증관련 단백질 및 유전자의 발현량을 확인하였다. LPS로 유도된 RAW 264.7 세포 내에서 시료의 nitric oxide 저해활성은 25 ㎍/ml에서 71.2 %의 우수한 효능을 나타내었으며, western blot 시험결과 iNOS, COX-2 단백질의 발현은 농도 의존적으로 감소하는 것으로 확인하였다. 이러한 실험 결과를 바탕으로 홍삼추출물이 항염 효과를 가진 화장품 소재로서의 가치를 제안할 수 있다.

Keywords

References

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