• Title/Summary/Keyword: saccharification

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Saccharification of Raw Starch in Ethanol Fermentation (에탄올발효에서 전분질무증자당화의 가능성연구)

  • Bae, Moo;Lee, Jae-Moon
    • Microbiology and Biotechnology Letters
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    • v.11 no.3
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    • pp.181-185
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    • 1983
  • The possibility of the ethanol fermentation from raw cassava starch without cooking was investigated. Saccharification yield in the simultaneous saccharification-fermentation (SSF) system was compared with that in saccharification of raw cassava starch, using glucoamylase of Aspergillus shirousmi. Although the saccharification yield of raw cassava starch with 10 folds of the enzyme was 60% compared to cooked cassava starch, higher saccharification could be obtained by SSF This result is maybe due to the elimination of end product inhibition in saccharification of raw starch by glucoamylase. Final ethanol yield from raw cassava starch was about 88% under the condition of 3$0^{\circ}C$, 120 rpm shaking after 3 days in the SSF system.

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Enhancing Enzymatic Saccharification by Aqueous Ammonia Soaking Pretreatment on Several annual plants (일년생 바이오매스(옥수수 줄기, 담배 줄기, 대마 목부)의 암모니아 침지 전처리가 효소 당화에 미치는 영향)

  • Shin, Soo-Jeong;Yu, Ju-Hyun;Park, Jong-Moon
    • 한국신재생에너지학회:학술대회논문집
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    • 2009.06a
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    • pp.418-421
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    • 2009
  • Effects of aqueous ammonia soaking to three annual plants (hemp woody core, tobacco stalk and corn stover) awere investigated to focus on the enzymatic saccharification characteristics change by this treatment. At two different levels of treatment ($90^{\circ}C$-16 h and $45^{\circ}C$-6 days), higher temperature treatment led to more enzymatic saccharification of cellulose to glucose by commercial cellulase mixtures (Celluclast 1.5L and Novozym 342 from Novozyme Korea). Difference among annual plants were significant. corn stover was the best response to enzymatic saccharification of cellulose and xylan by comercial enzymes all treatment conditions but tobacco stalk was the worst response to all of them. chemical composition or physical structure difference may brought this difference.

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Ethanol Production from Lignocellulosic Biomass by Simultaneous Saccharification and Fermentation Employing the Reuse of Yeast and Enzyme

  • KIM, JUN-SUK;KYUNG-KEUN OH;SEUNG-WOOK KIM;YONG-SEOB JEONG;SUK-IN HONG
    • Journal of Microbiology and Biotechnology
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    • v.9 no.3
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    • pp.297-302
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    • 1999
  • Simultaneous saccharification and fermentation (SSF) experiments were carried out with a lignocellulosic biomass. The effects of temperature on enzymatic saccharification and the ethanol fermentation were also investigated. The batch SSF process gave a final ethanol concentration of 10.44 g/l and equivalent glucose yield of 0.55 g/g, which was increased by 67% or higher over the saccharification at 42℃. The optimal operating condition was found to vary in several parameters, such as the transmembrane pressure, permeation rate, and separation coefficient, related to the SSF combined with membrane system (semi-batch system). When the fermentation was operated in a semi-batch mode, the efficiency of the enzymes and yeast lasted three times longer than in a batch mode.

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Cybernetic Modeling of Simultaneous Saccharification and Fermentation for Ethanol Production from Steam-Exploded Wood with Brettanomyces custersii

  • Shin Dong-Gyun;Yoo Ah-Rim;Kim Seung-Wook;Yang Dae-Ryook
    • Journal of Microbiology and Biotechnology
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    • v.16 no.9
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    • pp.1355-1361
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    • 2006
  • The simultaneous saccharification and fermentation (SSF) process consists of concurrent enzymatic saccharification and fermentation. In the present cybernetic model, the saccharification process, which is based on the modified Michaelis-Menten kinetics and enzyme inhibition kinetics, was combined with the fermentation process, which is based on the Monod equation. The cybernetic modeling approach postulates that cells adapt to utilize the limited resources available to them in an optimal way. The cybernetic modeling was suitable for describing sequential growth on multiple substrates by Brettanomyces custersii, which is a glucose- and cellobiose-fermenting yeast. The proposed model was able to elucidate the SSF process in a systematic manner, and the performance was verified by previously published data.

Saccharification and Sensory Characteristics of Shikhe made from Glutinous Rice Varieties (찹쌀 품종별 식혜의 당화 및 관능적 특성)

  • 신수영;성유미;강미영
    • Journal of the East Asian Society of Dietary Life
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    • v.11 no.1
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    • pp.11-18
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    • 2001
  • The saccharification and sensory characteristics of Sikhe, Korean traditional beverage of saccharified rice, made from thirteen glutinous rice varieties and one of non-glutinous rice named I1pum were examined. During saccharification, Shinsunchalbyeo, Whasunchalbyeo, Yukdononhrimna 1 and Hangangchalbyeo showed the highest sweetness determined by refractometer. Yukdononhrimna 1 was good for the morphology and texture of cooked rice kernel during saccharification. There was interrelationship between contents of released reducing sugar and sweetness determined by refractometer during preparation of Sikhe. The overall acceptability determined by sensory evaluation of Sikhe made from Yukdonongrimna 1, was slightly higher than that of Sikhe made from Ilpum, the non-glutionous rice variety. Yukdononhrimna 1 was considered to the most suitable varieties of Sikhe.

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Chemical Characterization of Industrial Hemp (Cannabis sativa) Biomass as Biorefinery Feedstock

  • Shin, Soo-Jeong;Han, Gyu-Seong;Choi, In-Gyu;Han, Sim-Hee
    • Korean Journal of Plant Resources
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    • v.21 no.3
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    • pp.222-225
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    • 2008
  • Chemical composition and enzymatic saccharification characteristics of hemp woody core were investigated by their chemical composition analysis and enzymatic saccharification with commercially available cellulases (Celluclast 1.5L and Novozym 342). Hemp woody core have higher xylan and lower lignin contents than its bast fiber. Based on hemicelluloses and lignin composition, hemp woody core is similar with hardwood biomass. However, cellulose was more easily converted to glucose than xylan to xylose and this trend was confirmed both hemp woody core and yellow poplar. Hemp woody core biomass shows higher saccharification than yellow poplar (hardwood biomass) based on cellulose and xylan hydrolysis. With easier enzymatic saccharification in cellulose and xylan, and similar chemical composition, hemp woody core have better biorefinery feedstock characteristics than hardwood biomass.

Effect of Sikhye Manufacturing Conditions on the Rice Shape (식혜제조 조건이 식혜밥알의 형태에 미치는 영향)

  • Kim, Su-Kweon;Kim, Joong-Man;Choi, Yong-Bae
    • Journal of the Korean Society of Food Culture
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    • v.15 no.1
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    • pp.1-8
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    • 2000
  • This study was to investigate the effects of concentration of malt extract in Sikhye manufacture on saccharification time, shapes of saccharified rice(the cooked rice) and sensory evaluation score of Sikhye. The optimum concentration of malt extract to reduce saccharification time and to keep desirable shapes of saccharified rice was 4 times (rice 24g, malt 28g and D.W. 240ml) as suitable as base composition formula(rice 6g, malt 7g and D.W. 240ml). The shapes of saccharified rice were influenced by the concentration of malt extract, the saccharification time and the shapes of steamed rice before saccharification. A good taste(softness) of Sikhye rice and the desirable shapes of saccharified rice were more suitable in the case of a small amount of unsaccharified starch than in the case of finishing saccharification. The optimum saccharification time to keep the desirable shapes of saccharified rice was 240min and also was desirable between 210 and 270min. To keep a good taste(softness) and the desirable shapes of saccharified rice, and to reduce the manufacturing time, it is desirable to in mass production of Sikhye add 3 times more water after making Sikhye in the ratio of rice 24g, malt 28g and water 240ml. In this case the whole amount will become 4 times as much as the original one.

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Monosaccharides from industrial hemp (Cannabis sativa L.) woody core pretreatment with ammonium hydroxide soaking treatment followed by enzymatic saccharification

  • Shin, Soo-Jeong;Han, Sim-Hee;Park, Jong-Moon;Cho, Nam-Seok
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.41 no.5
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    • pp.15-19
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    • 2009
  • Ammonia soaking treatment was introduced for hemp woody core pretreatment to increase enzymatic saccharification of polysaccharides. Portions of the xylan, cellulose, and lignin were removed by aqueous ammonia soaking, which improved the enzymatic saccharification of cellulose and xylan. Following ammonia soaking, 37% ($50^{\circ}C$-6 day treatment) to 61% ($90^{\circ}C$-16 h treatment) of the cellulose was converted to glucose and 33% ($50^{\circ}C$-6 day treatment) to 48% ($90^{\circ}C$-16 h treatment) of the xylan to xylose. Cellulose responded better to enzymatic saccharification than did xylan after the ammonia soaking treatment. Aqueous ammonia soaking pretreatment was more effective than electron beam irradiation for increasing enzymatic saccharification of xylan and cellulose in hemp woody core.

Liquefaction and Saccharification of Starch Using $\alpha$-Amylase and Immobilized Glucoamylase ($\alpha$-아밀레이즈와 고정화된 글루코아밀레이즈를 이용한 전분의 액화 및 당화)

  • 안대희;장호남
    • Microbiology and Biotechnology Letters
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    • v.19 no.5
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    • pp.497-503
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    • 1991
  • The catalytic activities of immobilized gIucoamylase in a packed bed column and a continuous stirred tank reactor have been compared. Rapid production of glucose from liquefied starch have been studied through, the continuous liquefaction and saccharification using settling chamber. The immobilized glucoamylase with chitin gave the saccharification yield of 20% with the dextrin concentration of 100 g/l in a residence of 20 min. in a packed bed column. The half-life of immobilized glucoamylase with chitin was 19 days. The glucoamyalse immobilized in chitin and encapsulated with Ca-alginate gave the saccharification yield of 6% with the dextrin concentration of 50 g/l in a residence of 20 min. in a packed bed column. The Ca-alginate encapsulated and chitin immobilized glucomylase had a half-life of 25 days, which is 6 day larger than that of the immobilized glucoamylase with chitin only. In continuous liquefaction and saccharification, the glucose yield was 17% for the liquefied starch with naked barley concentration of 50gA in a residence of 20 min.

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Quality characteristics of Sikhye prepared by different ratio of Seomaeyaksuk (Artemisia argyi H) extract (섬애약쑥 추출물의 첨가 비율에 따른 식혜의 품질특성)

  • Shin, Jeong Yeon;Woo, Yeon U;Jo, Yu Lim;Seo, Weon Taek;Choi, Jine Shang
    • Food Science and Preservation
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    • v.24 no.2
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    • pp.196-205
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    • 2017
  • This study was conducted to increase the functionality of Korean traditional beverage, Sikhye. The Sikhye were prepared by supplementation up to 20% of Seomaeyaksuk extract (SE) and saccharification for five hours (h). During the saccharification, turbidity of SE-added Sikhye (SES) was the highest at two hours of saccharification and then lowered gradually. L vlaue of SES did not show significant difference feduring saccharification, and both a and b value of Sikhye were increased as saccharification time passed and SE addition ratio was increased. The pH before saccharification was not significantly different by SE addition ratio but it was increased to 5.68-5.73 at 5 h saccharification. Soluble solid, total sugar and reducing sugar contents were enhanced as saccharification time and SE addition ratio were increased. The total phenolic compounds contents and DPPH radical scavenging activity showed a similar trend by saccharification time and SE addition. In sensory test, the evaluations of 5% SES and control were not significant different, but 20% SES group showed significant lower evaluation than other groups because strong color and mugwort flavor. From all of these results, physicochemical characteristics and antioxidant activity of SES was improved by SE addition but sensory characteristics was lower. Acceptable addition ratio of SE in Sikhye is determined to be within 15%.