Journal of Environmental Health Sciences (한국환경보건학회지)

Korean Society of Environmental Health (한국환경보건학회)
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Comparison of Human Blood Cadmium Concentrations using Graphite Furnace Atomic Absorption Spectrometry (GF-AAS) and Inductively Coupled Plasma-mass Spectrometry (ICP-MS)

흑연로 원자 흡광 광도기와 유도 결합 플라즈마 질량 분석기를 이용한 인체 혈중 카드뮴 농도 비교

  • Kwon, Jung-Yeon (Heavy metal exposure Environmental Health Center, Dong-A University) ;
  • Kim, Byoung-Gwon (Heavy metal exposure Environmental Health Center, Dong-A University) ;
  • Lim, Hyoun-Ju (Heavy metal exposure Environmental Health Center, Dong-A University) ;
  • Seo, Jeong-Wook (Heavy metal exposure Environmental Health Center, Dong-A University) ;
  • Kang, Min-Kyung (Heavy metal exposure Environmental Health Center, Dong-A University) ;
  • Kim, Yu-Mi (Heavy metal exposure Environmental Health Center, Dong-A University) ;
  • Hong, Young-Seoub (Heavy metal exposure Environmental Health Center, Dong-A University)
  • 권정연 (동아대학교 중금속 노출 환경보건센터) ;
  • 김병권 (동아대학교 중금속 노출 환경보건센터) ;
  • 임현주 (동아대학교 중금속 노출 환경보건센터) ;
  • 서정욱 (동아대학교 중금속 노출 환경보건센터) ;
  • 강민경 (동아대학교 중금속 노출 환경보건센터) ;
  • 김유미 (동아대학교 중금속 노출 환경보건센터) ;
  • 홍영습 (동아대학교 중금속 노출 환경보건센터)
  • Received : 2018.07.23
  • Accepted : 2018.08.20
  • Published : 2018.10.28
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Abstract

Objectives: The aims of this study were to compare concentrations and the correspondence of human blood cadmium by using graphite furnace atomic absorption spectrometry (GF-AAS) and inductively coupled plasma-mass spectrometry (ICP-MS), which are representative methods of heavy metal analysis. Methods: We randomly selected 79 people who agreed to participate in the research project. After confirming the linearity of the calibration curves for GF-AAS and ICP-MS, the concentrations of cadmium in a quality control standard material and blood samples were measured, and the correlation and the degree of agreement were compared. Results: The detection limit of ICP-MS (IDL: $0.000{\mu}g/L$, MDL: $0.06{\mu}g/L$) was lower than that of GF-AAS (IDL: $0.085{\mu}g/L$, MDL: $0.327{\mu}g/L$). The coefficient of variation of the quality control standard material showed stable values for both ICP-MS (clinchek-1: 5.35%, clinchek-2: 6.22%) and GF-AAS (clinchek-1: 7.92%, clinchek-2: 5.22%). Recovery was relatively high for both ICP-MS (clinchek-1: 95.1%, clinchek-2: 92.8%) and GF-AAS (clinchek-1: 91.4%, clinchek-2: 98.8%), with more than 90%. The geometric mean, median, and percentile of blood samples were all similar. The agreement of the two instruments compared with the bias of the analytical values found that about 81% of the analytical values were within ${\pm}30%$ of the deviation from the ideal reference line (y=0). As a result of the agreement limit, the value included in the confidence interval was about 94%, which shows high agreement. Conclusion: In this study, we confirmed there was no significant difference in concentrations of a quality control standard material and blood samples. Since ICP-MS showed lower concentrations than GF-AAS at concentrations below the method detection limit of GF-AAS, it is expected that more precise results will be obtained by analyzing blood cadmium with ICP-MS.

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