Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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TiO2 Nanoparticles from Baker's Yeast: A Potent Antimicrobial

  • Peiris, MMK (Department of Microbiology, Faculty of Medical Sciences, University of Sri Jayewardenepura) ;
  • Guansekera, TDCP (Department of Microbiology, Faculty of Medical Sciences, University of Sri Jayewardenepura) ;
  • Jayaweera, PM (Department of Chemistry, Faculty of Applied Sciences, University of Sri Jayewardenepura) ;
  • Fernando, SSN (Department of Microbiology, Faculty of Medical Sciences, University of Sri Jayewardenepura)
  • Received : 2018.07.09
  • Accepted : 2018.08.20
  • Published : 2018.10.28
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Abstract

Titanium dioxide ($TiO_2$) has wide applications in food, cosmetics, pharmaceuticals and manufacturing due to its many properties such as photocatalytic activity and stability. In this study, the biosynthesis of $TiO_2$ nanoparticles (NPs) was achieved by using Baker's yeast. $TiO_2$ NPs were characterized by X-ray Diffraction (XRD), UV-Visible spectroscopy, Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM) and Energy Dispersive X-ray analysis (EDX) studies. The XRD pattern confirmed the formation of pure anatase $TiO_2$ NPs. According to EDX data Ti, O, P and N were the key elements present in the sample. SEM and TEM revealed that the nanoparticles produced were spherical in shape with an average size of $6.7{\pm}2.2nm$. The photocatalytic activity of $TiO_2$ NPs was studied by monitoring the degradation of methylene blue dye when treated with $TiO_2$ NPs. $TiO_2$ NPs were found to be highly photocatalytic comparable to commercially available 21 nm $TiO_2$ NPs. This study is the first report on antimicrobial study of yeast-mediated $TiO_2$ NPs synthesized using $TiCl_3$. Antimicrobial activity of $TiO_2$ NPs was greater against selected Gram-positive bacteria and Candida albicans when compared to Gram-negative bacteria both in the presence or absence of sunlight exposure. $TiO_2$ NPs expressed a significant effect on microbial growth. The results indicate the significant physical properties and the impact of yeast-mediated $TiO_2$ N Ps as a novel antimicrobial.

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References

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