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http://dx.doi.org/10.4014/jmb.1807.07005

TiO2 Nanoparticles from Baker's Yeast: A Potent Antimicrobial  

Peiris, MMK (Department of Microbiology, Faculty of Medical Sciences, University of Sri Jayewardenepura)
Guansekera, TDCP (Department of Microbiology, Faculty of Medical Sciences, University of Sri Jayewardenepura)
Jayaweera, PM (Department of Chemistry, Faculty of Applied Sciences, University of Sri Jayewardenepura)
Fernando, SSN (Department of Microbiology, Faculty of Medical Sciences, University of Sri Jayewardenepura)
Publication Information
Journal of Microbiology and Biotechnology / v.28, no.10, 2018 , pp. 1664-1670 More about this Journal
Abstract
Titanium dioxide ($TiO_2$) has wide applications in food, cosmetics, pharmaceuticals and manufacturing due to its many properties such as photocatalytic activity and stability. In this study, the biosynthesis of $TiO_2$ nanoparticles (NPs) was achieved by using Baker's yeast. $TiO_2$ NPs were characterized by X-ray Diffraction (XRD), UV-Visible spectroscopy, Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM) and Energy Dispersive X-ray analysis (EDX) studies. The XRD pattern confirmed the formation of pure anatase $TiO_2$ NPs. According to EDX data Ti, O, P and N were the key elements present in the sample. SEM and TEM revealed that the nanoparticles produced were spherical in shape with an average size of $6.7{\pm}2.2nm$. The photocatalytic activity of $TiO_2$ NPs was studied by monitoring the degradation of methylene blue dye when treated with $TiO_2$ NPs. $TiO_2$ NPs were found to be highly photocatalytic comparable to commercially available 21 nm $TiO_2$ NPs. This study is the first report on antimicrobial study of yeast-mediated $TiO_2$ NPs synthesized using $TiCl_3$. Antimicrobial activity of $TiO_2$ NPs was greater against selected Gram-positive bacteria and Candida albicans when compared to Gram-negative bacteria both in the presence or absence of sunlight exposure. $TiO_2$ NPs expressed a significant effect on microbial growth. The results indicate the significant physical properties and the impact of yeast-mediated $TiO_2$ N Ps as a novel antimicrobial.
Keywords
Titanium dioxide; Yeast; X-ray diffraction; photocatalytic activity;
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