Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Effect of n-6/n-3 fatty acid ratio on metabolic partitioning in hyperlipidemic rats

n-6/n-3 지방산 비율이 고지혈증 랫드의 지질대사 분할에 미치는 영향

  • Lee, Seunghyung (College of Animal Life Science, Kangwon National University) ;
  • Um, Kyung-Hwan (College of Animal Life Science, Kangwon National University) ;
  • Park, Byung-Sung (College of Animal Life Science, Kangwon National University)
  • 이승형 (강원대학교 동물생명과학대학) ;
  • 엄경환 (강원대학교 동물생명과학대학) ;
  • 박병성 (강원대학교 동물생명과학대학)
  • Received : 2018.07.15
  • Accepted : 2018.09.18
  • Published : 2018.09.30
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Abstract

This study was investigated the mechanism of action of n-6/n-3 fatty acid ratio on the metabolic partitioning of blood glycerolipids by in vivo monitoring technique in hyperlipidemic animal model rats. The ratio of cholesteryl 14C-oleate metabolized in the liver of total glycerolipids was lower in the order of n-6/n-3 ratios of 4:1, 15:1, 30:1 and control group (p<0.05). The secretion amount of phospholipid was higher in the order of n-6/n-3 ratio 4:1, 15:1, 30:1 than the control (p<0.05). The secretion amount of triglyceride was lower in especially 4:1, in order of n-6/n-3 4:1, 15:1 and 30:1 compared with the control. The ratio of phospholipid partitioning to total glycerolipid was high in orfer of n-6/n-3 ratio 4:1, 15:1, 30:1 and control (p<0.05). The triacylglycerol partitioning (%) via liver was higher 72.97, 75.93, 78.12% in n-6/n-3 4;1, 15:1, 30:1, respectively than the control of 82.25%, according to increased n-6/n-3 (p<0.05). The phospholipid partitioning (%) was lower 25.15, 18.87, 18.15% in n-6/n-3 4;1, 15:1, 30:1, respectively, compared to control 11.04%, according to increased n-6/n-3 (p<0.05).

본 연구는 고지혈증 모델동물 랫드에서 n-6/n-3가 서로 다른 식이를 급여하였을 때 혈액지질의 대사적 분할에 미치는 작용 메카니즘을 생체 모니터링 기법으로 구명하였다. 총 glycerolipids의 간에서 대사된 cholesteryl $^{14}C$-oleate 비율은 n-6/n-3 비율 4:1, 15:1, 30:1, 대조군 순서로 낮았다(p<0.05). 인지질 분비량은 대조군과 비교할 때 n-6/n-3 비율 4:1, 15:1, 30:1 순서로 높았다(p<0.05). 중성지방 분비량은 대조군과 비교할 때 n-6/n-3 비율 4:1, 15:1, 30:1 순서로 특히, 4:1 처리군에서 낮았다(p<0.05). 총 glycerolipid에 대한 인지질의 분할 비율은 n-6/n-3 비율 4:1, 15:1, 30:1, 대조군 순서로 높았다(p<0.05). 간으로부터 중성지방 분할 비율(%)은 대조군 82.25%와 비교할 때 n-6/n-3 비율 4:1, 15:1, 30:1에서 각각 72.99, 75.93, 78.12%로써 n-6/n-3 비율이 증가할수록 높아졌다(p<0.05). 인지질 분할 비율(%)은 대조군 11.04%와 비교할 때 n-6/n-3 비율 4:1, 15:1, 30:1에서 각 25.15, 18.87, 18.15%로써 n-6/n-3 비율이 증가할수록 낮아졌다(p<0.05).

Keywords

References

  1. A. P. Simopoulos, "The importance of the ratio of omega-6/omega-3 essential fatty acids", Biomed. Pharmacother, Vol. 56, pp. 365-379, (2002). https://doi.org/10.1016/S0753-3322(02)00253-6
  2. A. P. Simopoulos, "An increase in the omega-6/omega-3 fatty acid ratio increases the risk for obesity", Nutrients, Vol. 8, pp. 128-135, (2016). https://doi.org/10.3390/nu8030128
  3. G. Schmitz, J. Ecker, "The opposing effects of n-3 and n-6 fatty acids", Prog. Lipid Res, Vol. 47, pp. 147-155, (2008). https://doi.org/10.1016/j.plipres.2007.12.004
  4. C. Gomez-Candela, L. M. Bermejo Lopez, V. Loria-Kohen, "Importance of a balanced omega-6/omega-3 ratio for the maintenance of health, Nutritional Recommendations", Nutr. Hosp, Vol. 26, pp. 323-329, (2011).
  5. K. S. Husted, E. V. Bouzinova, "The importance of n-6/n-3 fatty acids ratio in the major depressive disorder", Medicina, Vol. 52, pp. 139-147, (2016). https://doi.org/10.1016/j.medici.2016.05.003
  6. A. P. Simopoulos, "Importance of the ratio of omega-6/omega-3 essential fatty acids: evolutionary aspects", World Rev. Nutr. Diet, Vol. 92, pp. 1-22, (2003).
  7. A. P. Simopoulos, "The importance of the omega-6/omega-3 fatty acid ratio in cardiovascular disease and other chronic diseases", Exp. Biol. Med, Vol. 233, pp. 674-688, (2008). https://doi.org/10.3181/0711-MR-311
  8. M. C. Rudolph, B. E. Young, D. J. Lemas, C. E. Palmer, T. L. Hernandez, L. A. Barbour, J. E. Friedman, N. F. Krebs, P. S. MacLean, "Early infant adipose deposition is positively associated with the n-6 to n-3 fatty acid ratio in human milk independent of maternal BMI", Int. J. Obes, Vol. 41, pp. 510-517, (2017). https://doi.org/10.1038/ijo.2016.211
  9. B. M. Spiegelman, J. S. Flier, "Obesity and the regulation of energy balance", Cell, Vol. 104, pp. 531-543, (2001). https://doi.org/10.1016/S0092-8674(01)00240-9
  10. J. M. Friedman, "Modern science versus the stigma of obesity", Nat. Med, Vol. 10, pp. 563-569, (2004). https://doi.org/10.1038/nm0604-563
  11. S. M. Sternson, B. J. Nicholas, Z. F. Cao, "Neural circuits and motivational processes for hunger", Curr. Opin. Neurobiol, Vol. 23, pp. 353-360, (2013). https://doi.org/10.1016/j.conb.2013.04.006
  12. H. Q. Liu, Y. Qiu, Y. Mu, X. J. Zhang, L. Liu, X. H. Hou, L. Zhang, X. N. Xu, A. L. Ji, R. Cao, R. H. Yang, F. Wang, "A high ratio of dietary n-3/n-6 polyunsaturated fatty acids improves obesity-linked inflammation and insulin resistance through suppressing activation of TLR4 in SD rats", Nutr. Res, Vol. 33, pp. 849-858, (2013). https://doi.org/10.1016/j.nutres.2013.07.004
  13. L. I. Yang, Z. X. Song, W. Cao, Y. Y. Wang, H. X. Lu, F. Guo, H. Yang, J. Chen, S. K. Wang, G. J. Sun, "Effects of diets with different omega-6/omega-3 fatty acids on cardiovascular risk factors in mice fed high-fat diets", Wei Sheng Yan Jiu, Vol. 45, pp. 436-441, (2016).
  14. L. I. Yang, Z. X. Song, H. Yin, Y. Y. Wang, G. F. Shu, H. X. Lu, S. K. Wang, G. J. Sun, "Low omega-6/omega-3 PUFA ratio improves lipid metabolism, inflammation, oxidative stress and endothelial function in rats using plant oils as omega 3 fatty acid source", Lipids, Vol. 51, pp. 49-59, (2016). https://doi.org/10.1007/s11745-015-4091-z
  15. A. M. El-Badry, R. Graf, P. A. Clavien, "Omega 3-Omega 6: What is right for the liver?", J. Hepatol, Vol. 47, pp. 718-725, (2007). https://doi.org/10.1016/j.jhep.2007.08.005
  16. K. Bhardwaj, N. Verma, R. K. Trivedi, S. Bhardwaj, N. Shukla, "Significance of ratio of omega-3 and omega-6 in human health with special reference to flaxseed oil", Int. J. Biol. Chem, Vol. 10, pp. 1-6, (2016). https://doi.org/10.3923/ijbc.2016.1.6
  17. B. S. Park, "Effect of n-6/n-3 fatty acid ratio on lipid metabolism in obesity rats", J. Oil & Appl. Sci, In press, (2018).
  18. J. Hwangbo, S. O. Park, B. S. Park, "Lipid lowering mechanism of sulfur-fed grain larvae extract in high-fat induced obesity rats", J. Oil & Appl. Sci, Vol. 31, pp. 572-583, (2014).
  19. B. S. Park, "Effect of n-6/n-3 fatty acid ratio on hepatic glycerolipid partitioning in second generation rats", J. Oil & Appl. Sci, Vol. 34, pp. 839-850, (2017).
  20. P. G. Reeves, F. H. Nielsen, Jr, G. C. Fahey, "AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet", J. Nutr, Vol. 123, 1939-1951, (1993). https://doi.org/10.1093/jn/123.11.1939
  21. B. S. Park, "Method of biological monitoring related to lipid metabolism", Korean Patent, No. 10-1633979, (2016).
  22. B. S. Park, Y. C. Lee, "Studies on the partitioning of glycerolipid metabolism in pregnant and lactating rats", Kor. J. Anim. Sci, Vol. 39, pp. 705-714, (1995).
  23. A. M. B. Moir, V. A. Zammit, "Monitoring of changes in hepatic fatty acid and glycerolipid metabolism during the starved-to-fed transition in vivo, studies on awake, unrestrained rats", Biochem. J, Vol. 289, pp. 49-55, (1993). https://doi.org/10.1042/bj2890049
  24. V. A. Zammit, A. M Moir, "Monitoring the partitioning of hepatic fatty acids in vivo: keeping track of control", Trends Biochem. Sci, Vol. 19, pp. 313-317, (1994). https://doi.org/10.1016/0968-0004(94)90068-X
  25. V. A. Zammit, D. J. Lankester, A. M. Brown, B.S. Park, "Insulin stimulates triacylglycerol secretion by perfused livers from fed rats but inhibits it in livers from fasted or insulin-deficient rats implications for the relationship between hyperinsulinaemia and hypertriglyceridaemia", Eur. J. Biochem, Vol. 263, pp. 859-864, (1999). https://doi.org/10.1046/j.1432-1327.1999.00568.x
  26. M. L. Cruz, D. H. Williamson, "Refeeding meal-fed rats increases lipoprotein lipase activity and deposition of dietary[$^{14}C$] lipid in white adipose and decreases oxidation to $^{14}CO2$", Biochem. J, Vol. 285, pp. 773-778, (1992). https://doi.org/10.1042/bj2850773
  27. A.M. Moir, B.S. Park. V.A. Zammit, "Quantification in vivo of the effects of different types of dietary fat on the loci of control involved in hepatic triacylglycerol secretion", Biochem. J, Vol. 308, pp. 537-542, (1995). https://doi.org/10.1042/bj3080537
  28. L. Hodson, K. N. Frayn, "Hepatic fatty acid partitioning", Curr. Opin. Lipidol, Vol. 22, pp. 216-224, (2011). https://doi.org/10.1097/MOL.0b013e3283462e16
  29. M. L. Cruz, D. H. Williamson, "Refeeding meal-fed rats increases lipoprotein lipase activity and deposition of dietary[14C] lipid in white adipose and decreases oxidation to 14CO2", Biochem. J, Vol. 285, pp. 773-778, (1992). https://doi.org/10.1042/bj2850773