Browse > Article
http://dx.doi.org/10.12925/jkocs.2018.35.3.643

Effect of n-6/n-3 fatty acid ratio on metabolic partitioning in hyperlipidemic rats  

Lee, Seunghyung (College of Animal Life Science, Kangwon National University)
Um, Kyung-Hwan (College of Animal Life Science, Kangwon National University)
Park, Byung-Sung (College of Animal Life Science, Kangwon National University)
Publication Information
Journal of the Korean Applied Science and Technology / v.35, no.3, 2018 , pp. 643-653 More about this Journal
Abstract
This study was investigated the mechanism of action of n-6/n-3 fatty acid ratio on the metabolic partitioning of blood glycerolipids by in vivo monitoring technique in hyperlipidemic animal model rats. The ratio of cholesteryl 14C-oleate metabolized in the liver of total glycerolipids was lower in the order of n-6/n-3 ratios of 4:1, 15:1, 30:1 and control group (p<0.05). The secretion amount of phospholipid was higher in the order of n-6/n-3 ratio 4:1, 15:1, 30:1 than the control (p<0.05). The secretion amount of triglyceride was lower in especially 4:1, in order of n-6/n-3 4:1, 15:1 and 30:1 compared with the control. The ratio of phospholipid partitioning to total glycerolipid was high in orfer of n-6/n-3 ratio 4:1, 15:1, 30:1 and control (p<0.05). The triacylglycerol partitioning (%) via liver was higher 72.97, 75.93, 78.12% in n-6/n-3 4;1, 15:1, 30:1, respectively than the control of 82.25%, according to increased n-6/n-3 (p<0.05). The phospholipid partitioning (%) was lower 25.15, 18.87, 18.15% in n-6/n-3 4;1, 15:1, 30:1, respectively, compared to control 11.04%, according to increased n-6/n-3 (p<0.05).
Keywords
Omega 6 to omega 3; rats; hyperlipidemic; jugular vein; cannula; glycerolipid; metabolic partitioning;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 A. P. Simopoulos, "An increase in the omega-6/omega-3 fatty acid ratio increases the risk for obesity", Nutrients, Vol. 8, pp. 128-135, (2016).   DOI
2 J. M. Friedman, "Modern science versus the stigma of obesity", Nat. Med, Vol. 10, pp. 563-569, (2004).   DOI
3 S. M. Sternson, B. J. Nicholas, Z. F. Cao, "Neural circuits and motivational processes for hunger", Curr. Opin. Neurobiol, Vol. 23, pp. 353-360, (2013).   DOI
4 H. Q. Liu, Y. Qiu, Y. Mu, X. J. Zhang, L. Liu, X. H. Hou, L. Zhang, X. N. Xu, A. L. Ji, R. Cao, R. H. Yang, F. Wang, "A high ratio of dietary n-3/n-6 polyunsaturated fatty acids improves obesity-linked inflammation and insulin resistance through suppressing activation of TLR4 in SD rats", Nutr. Res, Vol. 33, pp. 849-858, (2013).   DOI
5 L. I. Yang, Z. X. Song, W. Cao, Y. Y. Wang, H. X. Lu, F. Guo, H. Yang, J. Chen, S. K. Wang, G. J. Sun, "Effects of diets with different omega-6/omega-3 fatty acids on cardiovascular risk factors in mice fed high-fat diets", Wei Sheng Yan Jiu, Vol. 45, pp. 436-441, (2016).
6 L. I. Yang, Z. X. Song, H. Yin, Y. Y. Wang, G. F. Shu, H. X. Lu, S. K. Wang, G. J. Sun, "Low omega-6/omega-3 PUFA ratio improves lipid metabolism, inflammation, oxidative stress and endothelial function in rats using plant oils as omega 3 fatty acid source", Lipids, Vol. 51, pp. 49-59, (2016).   DOI
7 A. M. El-Badry, R. Graf, P. A. Clavien, "Omega 3-Omega 6: What is right for the liver?", J. Hepatol, Vol. 47, pp. 718-725, (2007).   DOI
8 K. Bhardwaj, N. Verma, R. K. Trivedi, S. Bhardwaj, N. Shukla, "Significance of ratio of omega-3 and omega-6 in human health with special reference to flaxseed oil", Int. J. Biol. Chem, Vol. 10, pp. 1-6, (2016).   DOI
9 B. S. Park, "Effect of n-6/n-3 fatty acid ratio on lipid metabolism in obesity rats", J. Oil & Appl. Sci, In press, (2018).
10 B. S. Park, "Effect of n-6/n-3 fatty acid ratio on hepatic glycerolipid partitioning in second generation rats", J. Oil & Appl. Sci, Vol. 34, pp. 839-850, (2017).
11 P. G. Reeves, F. H. Nielsen, Jr, G. C. Fahey, "AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet", J. Nutr, Vol. 123, 1939-1951, (1993).   DOI
12 B. S. Park, "Method of biological monitoring related to lipid metabolism", Korean Patent, No. 10-1633979, (2016).
13 B. S. Park, Y. C. Lee, "Studies on the partitioning of glycerolipid metabolism in pregnant and lactating rats", Kor. J. Anim. Sci, Vol. 39, pp. 705-714, (1995).
14 A. M. B. Moir, V. A. Zammit, "Monitoring of changes in hepatic fatty acid and glycerolipid metabolism during the starved-to-fed transition in vivo, studies on awake, unrestrained rats", Biochem. J, Vol. 289, pp. 49-55, (1993).   DOI
15 V. A. Zammit, A. M Moir, "Monitoring the partitioning of hepatic fatty acids in vivo: keeping track of control", Trends Biochem. Sci, Vol. 19, pp. 313-317, (1994).   DOI
16 V. A. Zammit, D. J. Lankester, A. M. Brown, B.S. Park, "Insulin stimulates triacylglycerol secretion by perfused livers from fed rats but inhibits it in livers from fasted or insulin-deficient rats implications for the relationship between hyperinsulinaemia and hypertriglyceridaemia", Eur. J. Biochem, Vol. 263, pp. 859-864, (1999).   DOI
17 L. Hodson, K. N. Frayn, "Hepatic fatty acid partitioning", Curr. Opin. Lipidol, Vol. 22, pp. 216-224, (2011).   DOI
18 M. L. Cruz, D. H. Williamson, "Refeeding meal-fed rats increases lipoprotein lipase activity and deposition of dietary[$^{14}C$] lipid in white adipose and decreases oxidation to $^{14}CO2$", Biochem. J, Vol. 285, pp. 773-778, (1992).   DOI
19 J. Hwangbo, S. O. Park, B. S. Park, "Lipid lowering mechanism of sulfur-fed grain larvae extract in high-fat induced obesity rats", J. Oil & Appl. Sci, Vol. 31, pp. 572-583, (2014).
20 A.M. Moir, B.S. Park. V.A. Zammit, "Quantification in vivo of the effects of different types of dietary fat on the loci of control involved in hepatic triacylglycerol secretion", Biochem. J, Vol. 308, pp. 537-542, (1995).   DOI
21 M. L. Cruz, D. H. Williamson, "Refeeding meal-fed rats increases lipoprotein lipase activity and deposition of dietary[14C] lipid in white adipose and decreases oxidation to 14CO2", Biochem. J, Vol. 285, pp. 773-778, (1992).   DOI
22 M. C. Rudolph, B. E. Young, D. J. Lemas, C. E. Palmer, T. L. Hernandez, L. A. Barbour, J. E. Friedman, N. F. Krebs, P. S. MacLean, "Early infant adipose deposition is positively associated with the n-6 to n-3 fatty acid ratio in human milk independent of maternal BMI", Int. J. Obes, Vol. 41, pp. 510-517, (2017).   DOI
23 G. Schmitz, J. Ecker, "The opposing effects of n-3 and n-6 fatty acids", Prog. Lipid Res, Vol. 47, pp. 147-155, (2008).   DOI
24 C. Gomez-Candela, L. M. Bermejo Lopez, V. Loria-Kohen, "Importance of a balanced omega-6/omega-3 ratio for the maintenance of health, Nutritional Recommendations", Nutr. Hosp, Vol. 26, pp. 323-329, (2011).
25 K. S. Husted, E. V. Bouzinova, "The importance of n-6/n-3 fatty acids ratio in the major depressive disorder", Medicina, Vol. 52, pp. 139-147, (2016).   DOI
26 A. P. Simopoulos, "Importance of the ratio of omega-6/omega-3 essential fatty acids: evolutionary aspects", World Rev. Nutr. Diet, Vol. 92, pp. 1-22, (2003).
27 A. P. Simopoulos, "The importance of the omega-6/omega-3 fatty acid ratio in cardiovascular disease and other chronic diseases", Exp. Biol. Med, Vol. 233, pp. 674-688, (2008).   DOI
28 B. M. Spiegelman, J. S. Flier, "Obesity and the regulation of energy balance", Cell, Vol. 104, pp. 531-543, (2001).   DOI
29 A. P. Simopoulos, "The importance of the ratio of omega-6/omega-3 essential fatty acids", Biomed. Pharmacother, Vol. 56, pp. 365-379, (2002).   DOI