KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
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Quantification of Isoflavone Malonylglucosides in Soybean Seed during Germination

콩 발아 중 isoflavone malonylglucosides의 함량 변이

  • Lee, Ju-Won (Dept. of Biotechnology, SoonChunHyang Univ.) ;
  • Yi, Yoo-Jung (Dept. of Biotechnology, SoonChunHyang Univ.) ;
  • Lee, Ju-Hee (Dept. of Biotechnology, SoonChunHyang Univ.) ;
  • Jo, Min-Sik (Dept. of Biotechnology, SoonChunHyang Univ.) ;
  • Choi, Do-Jin (Dept. of Biotechnology, SoonChunHyang Univ.) ;
  • Ma, Mu-Hyun (Dept. of Biotechnology, SoonChunHyang Univ.) ;
  • Kim, Hong-Sik (Dept. of Southern Area Crop Science, NICS, RDA) ;
  • Kim, Dae-Ok (Dept. of Food Science and Biotechnolooy, KyungHee Univ.) ;
  • Yun, Hong-Tae (Dept. of Central Area Crop Science, NICS, RDA) ;
  • Kim, Yong-Ho (Dept. of Biotechnology, SoonChunHyang Univ.)
  • 이주원 (순천향대학교 의료생명공학과) ;
  • 이유정 (순천향대학교 의료생명공학과) ;
  • 이주희 (순천향대학교 의료생명공학과) ;
  • 조민식 (순천향대학교 의료생명공학과) ;
  • 최도진 (순천향대학교 의료생명공학과) ;
  • 마무현 (순천향대학교 의료생명공학과) ;
  • 김홍식 (국립식량과학원 남부작물부) ;
  • 김대옥 (경희대학교 식품생명공학과) ;
  • 윤홍태 (국립식량과학원 중부작물부) ;
  • 김용호 (순천향대학교 의료생명공학과)
  • Received : 2018.04.30
  • Accepted : 2018.06.24
  • Published : 2018.09.30
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Abstract

Soybean produces three major types of isoflavones, daidzein, genistein, and glycitein aglycones and their glucosides and malonylglucosides. It has been known that malonylated glucosides are rapidly converted to their corresponding aglycones due to the unstable thermolabile glucoside malonates; therefore, the analytical study of malonylated glucosides has been insufficient. In this study, we analyzed the malonylglucoside content in soybean seeds. Isoflavone analysis of three soybean cultivars revealed that 81.5~90.0% of the total isoflavones were malonylglucosides, whereas aglycones were rarely detected. Moreover, the total isoflavone content increased during a 5-day germination period where growth regulators and coumaric acid treatments tended to yield higher isoflavone content than the normal germination treatment, however the differences were not significant; notably, the isoflavone accumulation trend continued with additional germination days. The content of malonylglucoside was higher than that of other isoflavones, which was 83.7~86.6% of the total isoflavone content in seeds with a 3-day germination period. Furthermore, isoflavones were significantly accumulated in the hypocotyl of seedlings with a 5-day germination period. The content of isoflavone in the hypocotyl of the Pungsannamul-kong was 10,240 ug/g when treated with coumaric acid, which was considerably higher than that of other cultivars and treatments. Additionally, soybean seeds heated at $60^{\circ}C$ for 1 hour produced higher isoflavone content than non-heated soybean seeds. Our results show that it is possible to increase the isoflavone content in soybean seeds through various treatments.

콩 발아기간 동안 이소플라본 함량 증가를 위하여 생장조절제(GA, NAA, BA) 및 쿠마르산 처리를 하였다. 공시재료는 2016년에 재배된 대풍2호, 우람콩, 풍산나물콩 등 3품종을 사용하였다. 공시품종의 이소플라본 함량을 분석한 결과 우람 > 대풍 2호 > 풍산나물콩의 순으로 이소플라본 함량이 높았다. 이소플라본 종류별로는 malonylglucosides가 81.5~90.0%로 나타나 콩 이소플라본의 주된 성분임을 알 수 있었으며, 다음으로는 glucosides, acetylglucosides 순이었고 aglycone은 분석되지 않았다. 배당체로는 daidzin과 genistin이 분석된 반면 glycitin은 분석되지 않았고 acetylglucosides에서는 acetylglycitin만 분석되었다. 발아시간이 경과함에 따라 이소플라본 함량은 증가하였으며 식물생장조절제와 쿠마르산을 첨가한 경우도 마찬가지였다. 그런데 생장조절제와 쿠마르산 처리는 단순한 수분공급처리와 비교할 때 총 이소플라본 함량에서 처리 간 유의성은 없었으나 대체로 이소플라본 함량이 높게 분석되어졌다. 발아 5일차 배축에 이소플라본이 상당량 축적됨을 확인할 수 있었으며, 풍산나물콩의 경우 쿠마르산 처리 시 10,240 ug/g의 함량을 보여 다른 품종 및 처리에 비해 월등히 높았다. 발아 3일차 시료에서 이소플라본 종류별 함량을 분석한 결과 발아에 사용된 원료 콩 종자에서와 같이 malonylglucosides가 전체의 83.7~86.6%를 차지하여 가장 높았다. 또한 $60^{\circ}C$에서 1시간 열처리한 콩이 열처리하지 않은 콩보다 이소플라본 함량이 높아짐을 확인하였으며, 따라서 다양한 스트레스를 통하여 이소플라본 함량을 높일 수 있을 것으로 사료된다.

Keywords

References

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