Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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UV-induced skin damage improvement effects of solvent fraction from Maekmoondong-tang

맥문동탕 용매 분획물의 UV에 의한 피부손상 개선 효능평가

  • Yu, Jae-Myo (National Development Institute of Korean Medicine) ;
  • Kang, Yun-Hwan (National Development Institute of Korean Medicine) ;
  • Kim, Bo-Mi (National Development Institute of Korean Medicine) ;
  • Kim, Dong-Hee (National Development Institute of Korean Medicine) ;
  • Park, Tae-Soon (National Development Institute of Korean Medicine)
  • Received : 2018.07.27
  • Accepted : 2018.09.02
  • Published : 2018.09.29
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Abstract

In this study, we verified the 1-1-diphenyl-2-picryl-hydrazyl radical scavenge, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenge, elastase, tyrosinase inhibitory effect by using the solvent fractions of Maekmoondong-tang hot water extract. As a result, the ethyl acetate fraction (MW-EA) showed the highest inhibitory activity. In cell-based assays, MW-EA treatment confirmed a 34% ($100{\mu}g/mL$) efficacy in reactive oxygen species inhibitory activity, and at the same concentration, MMPs showed more than 50% inhibition and tyrosinase inhibited 25% ($50{\mu}g/mL$). Therefore Maekmoondong-tang is considered high development potential as a material to improve the skin.

맥문동탕 열수추출물의 용매분획물을 이용하여 1-1-diphenyl-2-picryl-hydrazyl radical scavenge, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenge, elastase, tyrosinase 억제 효과를 검증하였고 그 결과 에틸아세테이트 분획물(MW-EA)에서 가장 우수한 저해활성을 보여주었다. 세포를 이용한 활성 검증에서는 MW-EA를 처리하였을 때 ROS 저해활성에서 34% ($100{\mu}g/mL$), MMPs 저해 50% ($100{\mu}g/mL$) 이상, 미백 활성에서는 tyrosinase를 25% ($50{\mu}g/mL$) 억제하는 것을 확인 하였다. 따라서 맥문동탕은 피부개선 소재로서 개발 가능성이 높다고 사료된다.

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