Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

Control of Cucumber Downy Mildew Using Resistant Cultivars and Organic Materials

저항성 품종과 유기농업자재를 활용한 오이 노균병 방제

  • 김용기 (국립농업과학원 유기농업과) ;
  • 박소향 (국립농업과학원 유기농업과) ;
  • 엄다옴 (국립농업과학원 유기농업과) ;
  • 홍성준 (농촌진흥청 농촌지원국 재해대응과) ;
  • 조정래 (국립농업과학원 유기농업과) ;
  • 안난희 (국립농업과학원 유기농업과) ;
  • 심창기 (국립농업과학원 유기농업과) ;
  • 김민정 (국립농업과학원 유기농업과) ;
  • 박종호 (국립농업과학원 유기농업과) ;
  • 한은정 (국립농업과학원 유기농업과) ;
  • 고병구 (국립농업과학원 유기농업과)
  • Received : 2018.03.30
  • Accepted : 2018.05.02
  • Published : 2018.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

We selected eight resistant cultivars including 'Heukryongsamcheok', 'Heukgeumsolsamcheok' and 'Gangryeoksamcheok' showing high suppressive effect against cucumber downy mildew (CDM) through plastic film house and field trials in spring and autumn season in 2015. Of them, these three varieties, 'Heukryongsamcheok', 'Heukgeumsolsamcheok' and 'Gangryeoksamcheok' were used to evaluated suppressive effect against CDM by comparing disease severity (area under disease development progress curve, AUDPC) with those of three susceptible varieties under plastic film house condition. AUDPC of three resistant cultivars was in the range of 10.9 to 23.6, meanwhile those of three susceptible cultivars was in the range of 286.7 to 290.3. Consequently, we confirmed that cultivation of the selected resistant cultivars can reduce CDM in vivo. When disease severity (diseased leaf area) was investigated on a resistant cultivar ('Heukryongsamcheok') sprayed without any organic materials and susceptible cultivars sprayed single-, two times- and three times with Bordeaux mixture, sulfur-loess mixture and $Kocide^R$ (copper hydroxide), respectively, it was the lowest in the resistant cultivar plot compared to susceptible cultivar plots sprayed with any organic materials. In addition, we evaluated control effect of the selected resistant cultivar by comparing that of organic materials including lime sulfur mixture, Bordeaux mixture and garlic extract. As a result, Bordeaux mixture showed the highest control effect against CDM. AUDPC of Bordeaux mixture, resistant cultivar, lime sulfur, garlic extract and untreated control was 3.9, 10.6, 95.6, 24.9, and 258.7, respectively. Based on the above-mentioned results, we think that the resistant cultivars and Bordeaux mixture can be effectively used to control CDM as one of control measures under the farmhouse condition.

오이 노균병에 대한 저항성 품종을 선발하고자 자연발병되는 노지와 비닐하우스 내에서 봄 재배기간과 가을재배기간에 병 발생을 조사하여 모든 시험조건에서 낮은 발병을 보이는 8품종(흑룡삼척, 흑금솔삼척, 강력삼척 포함)을 저항성 품종으로 선발하였다. 저항성 정도를 평가하고자 이들 3품종(흑룡삼척, 흑금솔삼척, 강력삼척)을 선발하고 3종의 감수성 품종을 대조로 하여 비닐하우스 조건에서 발병 후 경과일수 별 병 진전 정도를 조사하여 병반하면적(Area under disease progress curve, AUDPC)을 산출한 결과, 감수성 품종에서는 286.7-290.3 범위의 높은 값을 보인 반면 저항성 품종에서는 10.9-23.6 범위의 낮은 값을 보여 감수성 품종에 비해 저항성 품종에서 병 진전이 현저히 억제됨을 확인하였다. 결과적으로 선발한 3종의 저항성 품종은 발병이 낮음을 확인하였다. 오이 저항성 품종 재배에 따른 유기농업자재 대체효과를 조사하기 위하여 비가림 시설 내에서 저항성 품종을 대비로 하여 감수성 품종을 공시하여 유기농 재배시 많이 사용되는 보르도액, 황토유황합제, 코사이드를 각각 1회, 2회, 3회 처리한 결과, 저항성 품종을 재배하는 것이 감수성 품종에 유기농업자재를 처리한 그 어떤 처리보다 노균병 발생이 현저히 적었다. 저항성 품종 및 유기농업자재 처리에 따른 오이 노균병 병 진전 억제효과를 평가하기 위하여 발병정도를 조사하여 병반하면적을 산출한 결과, 저항성 품종재배 시험구의 병반하면적값은 10.6인 반면, 감수성 품종에 석회보르도액처리구, 석회황합제처리구, 마늘추출액처리구 및 무처리구에서는 각각 3.5, 95.6, 24.9, 254.0을 나타냈다. 이상의 결과를 근거로 저항성 품종 3종 (흑룡삼척, 흑금솔삼척, 강력삼척) 및 석회보르도액은 농가에서 노균병 방제를 위하여 효과적으로 사용할 수 있을 것으로 사료된다.

Keywords

References

  1. Adam, C. 2008. Screening cucumber (Cucumis sativus) for resistance to downy mildew (Pseudoperonospora cubensis). MS thesis. North Carolina State University, Raleigh, NC, USA.
  2. Angelov, D., Georgiev, P. and Krasteva, L. 2000. Two races of Pseudoperonospora cubensis on cucumbers in Bulgaria. Acta Hort. 510: 81-84.
  3. Bains, S. B. 1991. Classification of cucurbit downy mildew lesions into district categories. J. Mycol. Plant Pathol. 21: 269-272.
  4. Bains, S. B. and Jhooty, J. S. 1976. Host-range and possibility of pathological races in Pseudoperonospora cubensiscause - cause of downy mildew of muskmelon. Indian Phytopathol. 29: 214-216.
  5. Barnes, W. C. and Epps, W. M. 1954. An unreported type of resistance to cucumber downy mildew. Plant Dis. Rep. 38: 620 (Abstract).
  6. Buloviene, V. and Surviliene, E. 2006. Effect of metrological condition on spread and intensity of Pseudoperonospora cubensis on cucumber. Sodininkyste ir Darzininkyste 25: 186-191.
  7. Call, A. D. 2010. Studies on resistance to downy mildew in cucumber (Cucumis sativus) caused by Pseudoperonospora cubensis. MS thesis. North Carolina State University, Raleigh, NC, USA.
  8. Call, A. D., Criswell, A. D. and Wehner, T. C. 2012a. Screening cucumber for resistance to downy mildew caused by Pseudoperonospora cubensis Rostov. Crop Sci. 52: 577-592.
  9. Call, A. D., Criswell, A. D., Wehner, T. C., Ando, K. and Grumet, R. 2012b. Resistance of cucumber cultivars to a new strain of cucurbit downy mildew. HortiScience 47: 171-178.
  10. Cavatorta, J., Moriarty, G., Hening, M., Glos, M., Kreitinger, M. and Munger, M. 2007. ‘Marketmore 97': a monoecious slicing cucumber inbred with multiple disease and insect resistance. HortScience 42: 707-709.
  11. Cohen, Y., Meron, I., Mor, N. and Zuriel. 2003. A new pathotype of Pseudoperonospora cubensis causing downy mildew in cucurbits in Israel. Phytoparasitica 31: 458-466. https://doi.org/10.1007/BF02979739
  12. Colucci, S. J. and Holmes, G. J. 2014. Downy mildew of cucurbits. The Plant health Instructors. DOI: 10.1094/PHI-I-2010-0825-01.
  13. Colucci, S. J., Wehner, T. C. and Holmes, G. J. 2006. The downy mildew epidemic of 2004 and 2005 in the eastern United States. Cucurbitaceae 2006: 403-411.
  14. Cooper, J. G. 2012. Cucurbit downy mildew (Pseudoperonospora cubensis): cucumber resistance. MS thesis. Virginia Polytechnic Institute and State University.
  15. Criswell, A. D., Call, A. D. and Wehner, T. C. 2010. Genetic control of downy mildew resistance in cucumber-a review. Rep. Cucurbit Genet. Coop. 33: 13-16.
  16. Holdsworth, W. L., Summers, C. F., Glos, M., Smart, C. D. and Marzourek, M. 2014. Development of downy mildew-resistant cucumber for late-season production in the Northeastern United States. HortScience 49: 10-17.
  17. Inaba, T., Morinaka, T. and Hamaya, E. 1986. Physiological races of Pseudoperonospora cubensis isolated from cucumber and muskmelon in Japan. Bull. Natl. Inst. Agro-Environ. Sci. 2: 35-43.
  18. Jenkins, S. F. and Wehner, T. C. 1983. A system for the measurement of foliar diseases of cucumber. Rep. Cucurbit Genet. Coop. 6: 10-12.
  19. Koh, Y. J. 1983. Epidemiological studies on the slow blasting type resistance of rice cultivars and yield measurement. MS thesis. Seoul National University.
  20. Kuepper, G. 2003. Downy mildew control in cucurbits. ATTRA 1-800-346-9140. USDA.
  21. Lebeda, A. 1992. Screening of wild cucumis species against downy mildew (Pseudoperonospora cubensis) isolates from cucumber. Phytoparasitica 20: 203-210. https://doi.org/10.1007/BF02980842
  22. Lebeda, A. and Cohen, Y. 2011. Cucurbit downy mildew (Pseudoperonospora cubensis)-biology, ecology, epidemiology, hostpathogen interaction and control. Eur. J. Plant Pathol. 129: 157-192. https://doi.org/10.1007/s10658-010-9658-1
  23. Lebeda, A. and Prasil, J. 1994. Susceptibility of Cucumis sativus cultivars to Pseudoperonospora cubensis. Acta Phytopathol. Entomol. Hung. 29: 89-94.
  24. Lee, J. S., Han, K. S., Lee, S. C. and Soh, J. W. 2013. Screening for resistance to downy mildew among major commercial cucumber varieties. Res. Plant Dis. 19: 188-195. https://doi.org/10.5423/RPD.2013.19.3.188
  25. Louis, S. T. 2012. Monsanto Company Launches New Downy Mildew Resistant Cucumber Hybrids. Monsanto company, Carly Scaduto.
  26. Ma, Q. and Cui, H. 1995. Histopathology of cucumber resistance to downy mildew. Rep. Cucurbit Genet. Coop. 18: 26-28.
  27. McGrath, M. T. 2006. Update on Managing Downy Mildew in Cucurbits. Cornell University.
  28. McGrath, M. T. 2014. Cucumber Varieties Evaluated for resistance to Downy Mildew on Long Island in 2014. Cornell University.
  29. McGrath, M. T., LaMarsh, K. and Menasha, S. 2012. Downy mildewresistant cucumber variety evaluation, New York-2012. Midwest Vegetables Trial Report for 2012. New York, NY, USA.
  30. Olczak-Woltman, H., Marcinkowska, J. and Niemirowicz-Szczytt, K. 2011. The genetic basis of resistance to downy mildew in Cucumis spp.-latest developments and prospects. J. Appl. Genet. 52: 249-255. https://doi.org/10.1007/s13353-011-0030-8
  31. Palti, J. 1974. The significance of pronounced divergence in the distribution of Pseudoperonospora cubensis on its crop host. Phytoparasitica 2: 109-115. https://doi.org/10.1007/BF02980294
  32. Palti, J. and Cohen, Y. 1980. Downy mildew of cucurbits: The fungus and its host, distribution, epidemiology and control. Phytoparasitica 8: 107-147.
  33. Petrov, L., Boogert, K., Sheck, L., Baider, A., Rubin, E. and Cohen, Y. 2000. Resistance to downy mildew, Pseudoperonospora cubensis, in cucumbers. Acta Hortic. 510: 203-210.
  34. Piesen, D. and Miria, S. 2014. Looking for Downy Mildew Resistant Cucumber, Melon and Squash Seed Stocks. Twin Oaks Seed Farm.
  35. Savory, E. A., Granke, L. L., Quesada-Ocampo, L. M., Varbanova, M., Hausbesk, M. K. and Day, B. 2011. The cucurbit downy mildew pathogen Pseudoperonospora cubensis. Mol. Plant Pathol. 12: 217-226. https://doi.org/10.1111/j.1364-3703.2010.00670.x
  36. Shetty, N. V., Wehner, T. C., Thomas, C. E., Doruchowski, R. W. and Shetty, K. P. V. 2002. Evidence for downy mildew races in cucumber tested in Asia, Europe, and North America. Sci. Hortic. 94: 231-239. https://doi.org/10.1016/S0304-4238(02)00013-4
  37. Sitterly, W. R. 1972. Breeding for disease resistance in cucumber. Ann. Rev. Phytopathol. 10: 471-490. https://doi.org/10.1146/annurev.py.10.090172.002351
  38. Thomas, C. E., Inaba, T. and Cohen, Y. 1987. Physiological specialization in Pseudoperonospora cubensis. Phytopathology 77: 1621-1624. https://doi.org/10.1094/Phyto-77-1621
  39. Wehner, T. C. and Shetty, N. V. 1997. Downy mildew resistance of the cucumber germplasm collection in North Carolina field test. Crop Sci. 37: 1331-1340. https://doi.org/10.2135/cropsci1997.0011183X003700040050x