Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Purification of Cold-adapted Protease from Janthinobacterium sp.

Janthinobacterium sp. 유래 저온 활성 프로테아제 정제

  • Kim, Hyun-do (Department of Biotechnology and Bioengineering, Interdisciplinary Program for Bioenergy & Biomaterials, Chonnam National University) ;
  • Choi, Jong-il (Department of Biotechnology and Bioengineering, Interdisciplinary Program for Bioenergy & Biomaterials, Chonnam National University)
  • 김현도 (전남대학교 생물공학과, 바이오에너지 및 바이오소재협동과정) ;
  • 최종일 (전남대학교 생물공학과, 바이오에너지 및 바이오소재협동과정)
  • Received : 2018.03.06
  • Accepted : 2018.03.09
  • Published : 2018.06.28
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Abstract

In this study, purification of cold-adapted protease from Janthinobacterium sp. was investigated. First, using gradient precipitation, protease was confirmed to be deposited in the 30-80% range of ammonium sulfate. Next, DEAE-Sepharose column was used for the binding of the protease under various conditions. The optimal binding condition was found to be pH 8.5 and flow rate of 30 ml/h. Under the optimal condition, the protease was purified with 29% recovery yield. This result can be useful for the purification of other cold-adapted protein.

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References

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