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http://dx.doi.org/10.4014/mbl.1802.02016

Purification of Cold-adapted Protease from Janthinobacterium sp.  

Kim, Hyun-do (Department of Biotechnology and Bioengineering, Interdisciplinary Program for Bioenergy & Biomaterials, Chonnam National University)
Choi, Jong-il (Department of Biotechnology and Bioengineering, Interdisciplinary Program for Bioenergy & Biomaterials, Chonnam National University)
Publication Information
Microbiology and Biotechnology Letters / v.46, no.2, 2018 , pp. 111-113 More about this Journal
Abstract
In this study, purification of cold-adapted protease from Janthinobacterium sp. was investigated. First, using gradient precipitation, protease was confirmed to be deposited in the 30-80% range of ammonium sulfate. Next, DEAE-Sepharose column was used for the binding of the protease under various conditions. The optimal binding condition was found to be pH 8.5 and flow rate of 30 ml/h. Under the optimal condition, the protease was purified with 29% recovery yield. This result can be useful for the purification of other cold-adapted protein.
Keywords
Purification; protease; Janthinobacterium;
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