DOI QR코드

DOI QR Code

네 가지 다른 온도가 흰줄숲모기(Aedes albopictus) 유충 생장에 미치는 영향

The effect of four different temperatures on the growth of Aedes albopictus larva

  • 나수미 (서울여자대학교 대학원 생명환경공학과) ;
  • 장혜지 (서울여자대학교 생명환경공학과) ;
  • 박소정 (서울여자대학교 생명환경공학과) ;
  • 이은영 (서울여자대학교 생명환경공학과) ;
  • 도지선 (서울여자대학교 생명환경공학과) ;
  • 홍승비 (서울여자대학교 생명환경공학과) ;
  • 이훈복 (서울여자대학교 생명환경공학과)
  • Na, Sumi (Department of Bio & Environmental Technology, Graduate School of Seoul Women's University) ;
  • Jang, Hyeji (Department of Bio & Environmental Technology, Seoul Women's University) ;
  • Park, Sojung (Department of Bio & Environmental Technology, Seoul Women's University) ;
  • Lee, Eunyoung (Department of Bio & Environmental Technology, Seoul Women's University) ;
  • Doh, Jiseon (Department of Bio & Environmental Technology, Seoul Women's University) ;
  • Hong, Seungbie (Department of Bio & Environmental Technology, Seoul Women's University) ;
  • Yi, Hoonbok (Department of Bio & Environmental Technology, Seoul Women's University)
  • 투고 : 2018.01.15
  • 심사 : 2018.05.11
  • 발행 : 2018.05.31

초록

본 연구는 기후변화로 모기 매개 질병이 확산되고 있는 범지구적 차원에서 주요 질병을 매개하는 흰줄숲모기(Aedes albopictus) 유충의 생장이 여러 가지 온도 조건에 따라 어떻게 변화하는지를 확인하기 위하여 수행되었다. 흰줄숲모기 유충 120개체를 채집하여 120개의 $20m{\ell}$ 유리용기(vial)에 한 개체씩 넣어 12개의 수조($17{\times}24{\times}18cm^3$)에 유리용기를 10개씩 분리하고, 3반복수의 수조를 4가지 온도($17^{\circ}C$, $21^{\circ}C$, $24^{\circ}C$, $28^{\circ}C$)로 조절된 배양기에 배치시켰다. 각 수조는 1령 3개체, 2령 3개체, 3령 2개체, 4령 2개체로 구성하여 흰줄숲모기 유충의 온도에 따른 우화율 및 생장 단계 별 평균 사망률, 온도에 따른 생장 단계별 최저 사망률, 온도에 따른 평균 수중 생존기간에 대한 연구를 27일 동안 진행하였다. 그 결과, 온도에 따른 평균 우화율은 $21^{\circ}C$에서 $20.00{\pm}5.77%$, $17^{\circ}C$, $24^{\circ}C$, $28^{\circ}C$에서 $3.33{\pm}3.33%$로 나타났다. 생장 단계별 평균 사망률은 1령이 $19.24{\pm}3.65%$, 2령이 $16.48{\pm}3.25%$, 3령이 $23.54{\pm}5.06%$, 4령이 $40.74{\pm}7.08%$로 나타났다. 온도에 따른 생장 단계별 최저 사망률은 1령이 $17^{\circ}C$에서 $13.33{\pm}6.67%$, 2령이 $21^{\circ}C$에서 $7.41{\pm}7.41%$, 3령이 $24^{\circ}C$에서 $10.74{\pm}6.43%$, 4령이 $28^{\circ}C$에서 $20.37{\pm}5.46%$로 나타났다. 온도에 따른 평균 수중 생존기간은 $17^{\circ}C$$26.33{\pm}0.67$일, $21^{\circ}C$$23.33{\pm}1.33$일, $24^{\circ}C$$20.00{\pm}2.52$일, $28^{\circ}C$$11.67{\pm}1.20$일로 나타났다. 전체적인 결과를 통해, 흰줄숲모기 유충의 생장은 $21^{\circ}C$가 가장 적절한 온도인 것으로 나타났으며, 생장 단계 중 4령에서 가장 많은 개체가 사망하는 것으로 나타났다. 직접적인 온도 설정을 이용한 흰줄숲모기 유충에 대한 연구 결과는 향후 모기 방제방법의 기초자료로 활용될 것으로 기대된다.

We investigated to know the growth patterns of Aedes albopictus larva at the four different temperature conditions. Each of 120 individuals was placed into a $20m{\ell}$ vial and 12 sets (a set of 10 vials) were separated into 12 water tanks ($17{\times}24{\times}18cm^3$). Each water tank was composed of 3 the $1^{st}$ instar, 3 the $2^{nd}$ instar, 2 the $3^{rd}$ instar, and 2 the $4^{th}$ instar. Three sets of water tanks were placed under the four different incubator temperatures ($17^{\circ}C$, $21^{\circ}C$, $24^{\circ}C$, $28^{\circ}C$). We found that the eclosion rates were $20.00{\pm}5.77%$ at $21^{\circ}C$ and $3.33{\pm}3.33%$ at other temperatures. For the mosquito larva mortality rate, $1^{st}$ instar was $19.24{\pm}3.65%$, $2^{nd}$ instar was $16.48{\pm}3.25%$, $3^{rd}$ instar was $23.54{\pm}5.06%$, and $4^{th}$ instar was $40.74{\pm}7.08%$. The lowest mortality rate in growth stages according to temperature was $13.33{\pm}6.67%$ at $17^{\circ}C$ in $1^{st}$ instar larva, $7.41{\pm}7.41%$ at $21^{\circ}C$ at $2^{nd}$ instar larva, $10.74{\pm}6.43%$ at $24^{\circ}C$ in $3^{rd}$ instar larva, and $20.37{\pm}5.46%$ at $28^{\circ}C$ in $4^{th}$ instar larva. The survival period of mosquitoes in underwater were $26.33{\pm}0.67days$ at $17^{\circ}C$, $23.33{\pm}1.33days$ at $21^{\circ}C$, $20.00{\pm}2.52days$ at $24^{\circ}C$, and $11.67{\pm}1.20days$ at $28^{\circ}C$. From our results the most effective temperature to the normal growth of mosquito larva was $21^{\circ}C$, and the highest mortality rate was shown at the $4^{th}$ instar stage of larva growth. Our results would provide the basic data for the mosquito larva's growth pattern.

키워드

참고문헌

  1. Costanza, R, Ralph, DA, Rudolf, DG, Stphen, F, Monica,G, Bruce, H, Karin, L, Shahid, N, Robert, VO, Jose, P, Robert, G, Raskin, PS, and Marjan, VDB (1997). The value of the world's ecosystem services and natural capital, Nature, 387, pp. 253-260. [DOI: 10.1038/387253a0]
  2. Heo, IH and Kwon, WT (2007). Temperature Change of Recent 10 Years (1996-2005) in Korea, J. of climate research, 2(2), pp. 79-93. [Korean Literature]
  3. IPCC (2007). Climate Change 2007: The Physical Science Basis. Cambridge University Press, Cambridge, United Kingdom and New York, NY, USA.
  4. Jang, JY, Jo, SH (2003). Evaluation of climate change impact on Korea and adaptation program, KEI Report. Ajou university.
  5. Kim, DJ (2009). The Climate Change Adaptation Strategies for Infectious Diseases, Health and welfare policy forum, 154, pp. 23-38. [Korean Literature]
  6. Kim, SH (2011). A study of the distribution and epidemiological surveillance of Culex triaeniorhynchus (Diptera: Culicidae), a vector of Japanese encephalitis in Korea; 1985-2009, Master's Thesis, Korea University.
  7. KOBAYASHI, KZ and WADA, YSTK (1984). The relation between nutrition of each larval stage and autogeny of adult mosquito, The Japan Society of Medical Entomology and Zoology, 35(1), pp. 57-62. https://doi.org/10.7601/mez.35.57
  8. Lee, DG (2006). Evaluation of major infectious disease-related mosquito control projects at public health centers, Kosin University.
  9. Lee, SY (2004). Our insect book. Yearimdang.
  10. Rozilawati, H, Masri, SM, Tanaselvi, K, Zairi, J, Nazni, WA and Lee, HL (2016). EFFECT OF TEMPERATURE ON THE IMMATURE DEVELOPMENT OF AEDES ALBOPICTUS SKUSE. SOUTHEAST ASIAN J. OF TROPICAL MEDICINE AND PUBLIC HEALTH, 47(4), 731-746.
  11. Seo, HJ (2017). Correlation Study between Vector Mosquito Density and Climatic Factors in Pyeongtaek-city, 2012-2016. Master's Thesis, Dankook Universiy.
  12. Shim, HS, Myeong, IS, Hong, SG, Lee, YG, Lee, SG, Lee, GS, Park HH, Choi, HS and Ko, HG (2012). Status and damage caused by emergent pests caused by climate change, The Korean Society of Pesticide Science, pp. 11-18. [Korean Literature]
  13. WHO (2017). http://www.who.int/csr/disease/zika/en/.
  14. Xue, RD, Smith, ML, Yi, HB and Kline, DL (2015). Field Evaluation of a Novel Mos-Hole Trap and Naphtha Compared with BG Sentinel Trap and Mosquito Magnet X Trap to Collect Adult Mosquitoes, J. of the American Mosquito Control Association, 31(1), pp. 110-112. [DOI: 10.2987/14-6425.1]
  15. Yi, HB, Devkota, BR, Yu, JS and Kim HJ (2014a). A research for an eco-friendly mosquito control by using a new mosquito trap (Mos-hole trap) in a cowshed in Yeoju of Korea, Animal Cells and Systems, 18(4), pp. 282-290. [DOI: 10.1080/19768354.2014.938697]
  16. YI, HB, DEVKOTA, BR, YU, JS, OH, KC, KIM, JH and KIM HJ (2014b). Effects of global warming on mosquitoes & mosquito-borne diseases and the new strategies for mosquito control, Entomological Research, 44(2014), pp. 215-235. [DOI: 10.1111/1748-5967.12084]