• Korean journal of applied entomology
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• v.63 no.1
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• pp.77-80
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• 2024

Aedes albopictus, a common species in the Republic of Korea, is internationally known as a major vector for various diseases, and it is well-adapted to urban environments. Recent insect outbreaks in urban areas, attributed to climate change and urban heat islands, have increased the necessity of researching the effects on mosquito populations. This study analyzed climate data from 25 Automatic Weather System (AWS) stations in Seoul, identifying urban areas with pronounced heat island effects and suburban areas with milder effects. Nine urban heat island conditions were established based on this analysis, under which the hatching rates of Ae. albopictus were examined. The results revealed an increase in hatching rates correlating with the intensity of the urban heat island effect. Regression analysis further indicated that this trend accelerates as the strength of the heat island effect increases. This study suggests that temperature variations resulting from urban heat island phenomena can significantly influence the hatching rates of Ae. albopictus.

• The Korean Journal of Pesticide Science
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• v.4 no.2
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• pp.18-20
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• 2000

Methanol extracts of nine native plants(Teraxacum platycerpum leaf, Pinus densiflora leaf, Artemisia prinseps, leaf, Allium tuberosum leaf, Cassia obtussifolia, whole plant, Sophora angestifolia root, Stemonae sessilifolia root, Lonicera japonica, stem, leaf, flower, and Clivia miniata leaf were tested for repellent activity against Aedes albopictus and Blateria germanica. The extracts of P. densiflora, T. platycerpum, A. tuberosum and L. japonica (flower) exhibited excellent repellent activity against A. albopictus, while only L. japonica (leaf) had some good activity against B. germanica.

• Korean journal of applied entomology
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• v.44 no.3 s.140
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• pp.243-249
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• 2005

We investigated the repellency of eight mosquito repellents against Culex pipiens pallens and Aedes albopictus, and the quantity of DEETs were analysed by using gas chromatography. Ae. albopictus showed higher bloodsucking efficacy than Cx. pipiens pallens of the two subjects mouse and human hands, and human hand was more efficacious method for mosquito bloodsucking efficacy than mouse. Among the eight repellents were tested with mouse as subjects for eight hours, product C, G, and H were high repellency than others. In the case of human hands as subject, product C was highly effective than above other products. The DEET quantities of eight repellants varied from 7.31 g to 38.9 g in 100 g contents and we ascertained that there was a correlation between mosquito repellency and the DEET quantities remained after the treatments. It was evidenced from the long term test with shirt piece, in this test the Ae. albopictus attack was increased when the DEET quantity decreased below 40%.

• Proceedings of the Korea Contents Association Conference
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• 2014.11a
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• pp.55-56
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• 2014

치쿤구니아열은 치쿤구니아 바이러스(chikungu- nya virus)에 감염된 매개 모기(열대숲모기 및 흰줄숲모기)에 물려 감염되는 급성 열성 질환으로 잠복기가 짧고 치료제가 없기 때문에 조기 진단이 매우 중요한 급성전염병이다. 아열대기후로 진입하는 우리나라에서도 흰줄숲모기가 자주 발견되기 때문에 이 질병으로부터 결코 자유롭지가 않다. 치쿤구니아 바이러스 감염을 진단하기 위한 진단키트를 개발하기 위해 먼저 타깃 유전자 부위 선정이 매우 중요하다. 본 연구에서는 생명정보학을 기반으로 이 바이러스 만을 검출할 수 있는 epitope를 예측하고자 한다. 이 바이러스의 capsid 유전자를 찾고 유사한 바이러스의 유전자들과 multiple alignment를 수행하여 이 바이러스만이 가지고 있는 독특한 부위를 추출하였다. 이후 ProtScale Tool 프로그램으로 선택한 단백질의 친수성(hydrophilicity), 접근성(accessi- bility), 유연성(flexibility), 회전(${\beta}$-turns) 등의 특성을 모두 만족하는 부위를 선별하여 진단키트 제작을 위한 epitope를 제시하고자 한다.

• Korean journal of applied entomology
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• v.61 no.2
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• pp.369-376
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• 2022

Five different methanol extracts of plants were evaluated as a mosquito repellent against Aedes albopictus in nude mice model. The volatile components of plants were analyzed with GC-MS. The effective dose was the highest in Nepeta cataria with 72.9, 83.7, 86.4, and 97.3% efficiencies at 10, 50, 100, and 200 ㎍/mL, respectively. Nepeta cataria, Actinidia polygama, and Artemisia annua extracts were the most effective in duration time test. And there was no difference compared to negative control in the low mosquito repellent efficiency from 30 minutes. As a result of analyzing the volatile components of each plant, a total of 28 components in Mentha suaveolens, 19 components in Actinidia polygama, 27 components in Artemisia annua, 26 components in Nepeta cataria, and 19 components in Taraxacum platycarpum were detected. Especially, nepetalactone known as an effective ingredient of a mosquito repellent was identified (27.95 mg/Kg) in one of volatile components of Nepeta cataria. Overall, our results provide the possibility to develop mosquito repellents material using natural plants which contain various volatile components.

• Journal of Wetlands Research
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• v.20 no.2
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• pp.155-160
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• 2018

We investigated to know the growth patterns of Aedes albopictus larva at the four different temperature conditions. Each of 120 individuals was placed into a $20m{\ell}$ vial and 12 sets (a set of 10 vials) were separated into 12 water tanks ($17{\times}24{\times}18cm^3$). Each water tank was composed of 3 the $1^{st}$ instar, 3 the $2^{nd}$ instar, 2 the $3^{rd}$ instar, and 2 the $4^{th}$ instar. Three sets of water tanks were placed under the four different incubator temperatures ($17^{\circ}C$, $21^{\circ}C$, $24^{\circ}C$, $28^{\circ}C$). We found that the eclosion rates were $20.00{\pm}5.77%$ at $21^{\circ}C$ and $3.33{\pm}3.33%$ at other temperatures. For the mosquito larva mortality rate, $1^{st}$ instar was $19.24{\pm}3.65%$, $2^{nd}$ instar was $16.48{\pm}3.25%$, $3^{rd}$ instar was $23.54{\pm}5.06%$, and $4^{th}$ instar was $40.74{\pm}7.08%$. The lowest mortality rate in growth stages according to temperature was $13.33{\pm}6.67%$ at $17^{\circ}C$ in $1^{st}$ instar larva, $7.41{\pm}7.41%$ at $21^{\circ}C$ at $2^{nd}$ instar larva, $10.74{\pm}6.43%$ at $24^{\circ}C$ in $3^{rd}$ instar larva, and $20.37{\pm}5.46%$ at $28^{\circ}C$ in $4^{th}$ instar larva. The survival period of mosquitoes in underwater were $26.33{\pm}0.67days$ at $17^{\circ}C$, $23.33{\pm}1.33days$ at $21^{\circ}C$, $20.00{\pm}2.52days$ at $24^{\circ}C$, and $11.67{\pm}1.20days$ at $28^{\circ}C$. From our results the most effective temperature to the normal growth of mosquito larva was $21^{\circ}C$, and the highest mortality rate was shown at the $4^{th}$ instar stage of larva growth. Our results would provide the basic data for the mosquito larva's growth pattern.

• Korean Journal of Environmental Biology
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• v.28 no.2
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• pp.64-68
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• 2010

The mosquito, Aedes albopictus is important vector of humen phatogen such as dengue fever. And Aedes albopictus has widly distribution in oriental regions. This study was conducted to investigate the biological effects of cooper on the development of the Asian tiger mosquitoes, Aedes albopictus (Skuse). Aedes albopictus was affected with pupation ratios, mortalities, and wing lengths of the 3th and 4th instars by exposure to different concentration $CuCl_2$ (0.0, 2.5, 25.0 and 50.0 ppm) for 24 h and 48 h depending on dose and exposure period. The lowest $LC_{50}$ (median lethal concentration) value of $CuCl_2$ was 18.1 ppm at the 3rd instars for 48 h exposure. The pupation ratio of the larvae exposed at 50.0 ppm for 48 h was 14% which was much lower than those exposed to other treatment groups Changes of adult wing length showed significant decreased by Cu treatment. In conclusion, the most prominent effects of high concentration and longer exposure period were to reduce in the survival and pupation rates of larvae and wing length of Ae. albopictus.

• Korean Journal of Clinical Laboratory Science
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• v.51 no.3
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• pp.336-343
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• 2019

In order to investigate the density of seasonal incidence of mosquitoes, a vector of infectious diseases in Jeju region, this study collected mosquitoes using a Black light trap (BL) and Biogents' Sentinel 2 Mosquito Trap (BG), dividing the region into cattle sheds, habitats for migratory birds, and the downtown area, twice a month for 9 months from March through November 2018. Then, this study conducted identification and classification and checked for the presence of Flavivirus using reverse transcription polymerase chain reaction (RT-PCR). As for the mosquito population, 1,847 mosquitoes (six genera, 12 species) were collected. The places where most mosquitoes were collected were copses near craft workshops in habitats for migratory birds and Jungang-dong in the Seogwipo downtown area. For the population, Culex pipiens pallens was the dominant species (76.9%), followed by Aedes albopictus (8.9%). Most of the population was collected in June, followed by August and October. This study conducted a RT-PCR test with 1,847 collected mosquitoes, which were divided into 50 pools if they had Flavivirus. All turned out to be negative. However, the results of the investigation show the presence of Culex tritaineniorhychus, Aedes albopictus, and Anopheles sinensis and can be used as a basis for the comprehensive prevention management of mosquitoes.

• Korean journal of applied entomology
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• v.57 no.3
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• pp.177-183
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• 2018

This study examined the seasonal, regional distribution of mosquito vectors related with disease vectors in the Jeju. From March to November, sample were collected from 11 points in four environmentally different sites in Jeju Island. Samples were collected twice a month using a black-light trap and a BG sentinel trap. Overall, five genera and seven species types of 6,042 female mosquitos were collected. Among the collected mosquitos, 4,159 (68.8%) and 1,348 (24.4%) were Culex pipiens and Aedes albopictus, respectively, making them the dominant species. Additionally, collection using the black light trap produced 72.8 entities per trap in Jungang-dong service center in the center of the city, which was the highest value, while the lowest amount of 1.4 per trap was recovered from the airport. When the BG sentinel trap was used, the largest recovery was observed in the port, where there were 71.7 entities per trap, while the lowest amount of 28.3 entities per trap was recovered at Gealmae Eco Park. The overall number of mosquitoes collected started to increase from May, and reached the largest value of 1,156 (19.1%) in August. Trapped mosquitoes are created 364 pools of up to 50 grains per pool, by season, by environmental, and by species. When the pools were used, no flaviviral infection was observed upon real time RT-PCR.

• Korean journal of applied entomology
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• v.57 no.4
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• pp.381-392
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• 2018

The distribution of mosquitoes collected in the Cheongju area from 2016-2018 were compared. The collection sites were established at a residential area, migratory bird sanctuary, and cowshed using black light (BL) and BG sentinel traps. Culex pipiens was the most collected species in the residential area, and Aedes vexans was the most collected species in the migratory bird sanctuary and cowshed in all years. The BG trap collected more individuals than did the BL trap in the residential area and migratory bird sanctuary. In total, 22,679 (10 species) mosquitoes were collected in 2016, and 6,502 (8 species) and 6,803 (9 species) mosquitoes were collected in 2017 and 2018, accounting for 3.49- and 3.33-fold decreases, respectively. The relationship between meteorological conditions and mosquito density, according to various variables, was not significant different. The Chaoyang virus was found in Aedes vexans and Culex pipiens collected at the cowshed in 2016 and 2018, respectively. Based on the results of this study, it is considered that continuous surveillance of mosquitoes should be performed for controlling mosquito populations and mediating diseases spread by this vector.