대한본초학회지 (The Korea Journal of Herbology)

  • 제33권3호
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  • Pages.37-43
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  • 2018
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  • 1229-1765(pISSN)
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  • 2288-7199(eISSN)
대한본초학회 (The Korea Association of Herbology)
권호 표지
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차전초 에탄올 추출물의 항산화 효능

Antioxidant effect of ethanol extract from Plantaginis Herba

  • 김유진 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김소영 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 정미진 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 이은탁 (농업회사법인 (주)이비채) ;
  • 추성태 (농업회사법인 (주)이비채) ;
  • 윤석나 (대구한의대학교 대학원 보건학과) ;
  • 김미려 (대구한의대학교 한의과대학 본초약리학교실)
  • Kim, Yoo-Jin (Department of Herbal Pharmacology, Daegu Hanny University) ;
  • Kim, So Young (Department of Herbal Pharmacology, Daegu Hanny University) ;
  • Jeong, Mi Jin (Department of Herbal Pharmacology, Daegu Hanny University) ;
  • Lee, Un-Tak (Ebiche co., Ltd) ;
  • Choo, Sung-Tae (Ebiche co., Ltd) ;
  • Youn, Seok Na (Department of Public Health, Graduate School, Daegu Haany University) ;
  • Kim, Mi Ryeo (Department of Herbal Pharmacology, Daegu Hanny University)
  • 투고 : 2018.04.11
  • 심사 : 2018.05.25
  • 발행 : 2018.05.30
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초록

Objectives : Butylated hydroxytoluene (BHT) and butylated hydroxyanisole (BHA) were well known as anti-oxidant, but they were limited to use because of toxicity. So, many studies are being done to develope natural anti-oxidant. Total phenolic and flavonoid contents along with total antioxidant capacity of the ethanolic extract of Plantaginis Herba (PH) were evaluated to explore the reliable and potential sources of novel natural antioxidants. Methods : Total polyphenol contents and total flavonoid contents in PH ethanol extract were determined by colorimetric method. And DPPH(1.1-diphenyl-2-picrylhydrazyl), ABTS(2'-azino-bis (3-ethylbenzothiazoline-6-Surfonicacid)) free radical scavenging capacity and reducing power inhibition activities of PH ethanol extract were measured at 100, 500, 1000, $5000{\mu}g/m{\ell}$ concentrations by spectrometric assay. Results : The total polyphenol contents and total flavonoid contents of the extract were 161.99 mg/g, 144.05 mg/g, respectively. Also, DPPH, ABTS free radical scavenging capacity and reducing power of PH ethanol extract in treated concentrations (100, 500, 1000, $5000{\mu}g/m{\ell}$) increased dose dependently. In particular, DPPH free radical scavenging capacity of PH ethanol extract from $500{\mu}g/m{\ell}$ was significantly increased compared to positive control (BHA). ABTS free radical scavenging capacity of PH ethanol extract from $1000{\mu}g/m{\ell}$ was significantly higher than BHA. Also, reducing power showed that PH ethanol extract from $500{\mu}g/m{\ell}$ was significantly increased compared to BHA. Conclusions : These results suggest that PH ethanol extract has effects to scavenge free radicals, thus PH has potential and applicable benefits for development of materials and products to have anti-oxidation functions.

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