Browse > Article
http://dx.doi.org/10.6116/kjh.2018.33.3.37.

Antioxidant effect of ethanol extract from Plantaginis Herba  

Kim, Yoo-Jin (Department of Herbal Pharmacology, Daegu Hanny University)
Kim, So Young (Department of Herbal Pharmacology, Daegu Hanny University)
Jeong, Mi Jin (Department of Herbal Pharmacology, Daegu Hanny University)
Lee, Un-Tak (Ebiche co., Ltd)
Choo, Sung-Tae (Ebiche co., Ltd)
Youn, Seok Na (Department of Public Health, Graduate School, Daegu Haany University)
Kim, Mi Ryeo (Department of Herbal Pharmacology, Daegu Hanny University)
Publication Information
The Korea Journal of Herbology / v.33, no.3, 2018 , pp. 37-43 More about this Journal
Abstract
Objectives : Butylated hydroxytoluene (BHT) and butylated hydroxyanisole (BHA) were well known as anti-oxidant, but they were limited to use because of toxicity. So, many studies are being done to develope natural anti-oxidant. Total phenolic and flavonoid contents along with total antioxidant capacity of the ethanolic extract of Plantaginis Herba (PH) were evaluated to explore the reliable and potential sources of novel natural antioxidants. Methods : Total polyphenol contents and total flavonoid contents in PH ethanol extract were determined by colorimetric method. And DPPH(1.1-diphenyl-2-picrylhydrazyl), ABTS(2'-azino-bis (3-ethylbenzothiazoline-6-Surfonicacid)) free radical scavenging capacity and reducing power inhibition activities of PH ethanol extract were measured at 100, 500, 1000, $5000{\mu}g/m{\ell}$ concentrations by spectrometric assay. Results : The total polyphenol contents and total flavonoid contents of the extract were 161.99 mg/g, 144.05 mg/g, respectively. Also, DPPH, ABTS free radical scavenging capacity and reducing power of PH ethanol extract in treated concentrations (100, 500, 1000, $5000{\mu}g/m{\ell}$) increased dose dependently. In particular, DPPH free radical scavenging capacity of PH ethanol extract from $500{\mu}g/m{\ell}$ was significantly increased compared to positive control (BHA). ABTS free radical scavenging capacity of PH ethanol extract from $1000{\mu}g/m{\ell}$ was significantly higher than BHA. Also, reducing power showed that PH ethanol extract from $500{\mu}g/m{\ell}$ was significantly increased compared to BHA. Conclusions : These results suggest that PH ethanol extract has effects to scavenge free radicals, thus PH has potential and applicable benefits for development of materials and products to have anti-oxidation functions.
Keywords
Anti-oxidant; Plantaginis Herba; free radical scavenging capacity; total polyphenols; total flavonoids;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Arora A, Nair G, Strasburg GM. Structure activity relationships for antioxidant activities of a series of flavonoids in a liposomal system. Free Radic Biol Med. 1998 ; 24 : 1355-1363.   DOI
2 Rice-evans CA, Miller N, Paganga G. Antioxidant properties of phenolic compounds. Trends Plant Sci. 1997 ; 2(4) : 152-159.   DOI
3 Yusof S, Ghazali HM, King GS. Naringin content in local citrus fruits. Food Chem. 1990 ; 37 : 113-121.   DOI
4 Hermann K. Occurrence and content of hydroxycinnamic and hydroxyl-benzoic acid compounds in foods. Crit Rev Food Sci Nutr. 1989 ; 28 : 315-347.   DOI
5 Tzeng TF, Liu WY, Liou SS, Hong TY, Liu IM. Antioxidant-Rich Extract from Plantaginis Semen Ameliorates Diabetic Retinal Injury in a Streptozotocin-Induced Diabetic Rat Model. Nutrients. 2016 ; 8(9) : 18.   DOI
6 Liu YL, Tang LH, Liang ZQ, You BG, Yang SL: Growth inhibitory and apoptosis inducing by effects of total flavonoids from Lysimachia clethroides Duby in human chronic myeloid leukemia K562 cells. J Ethnopharmacol .2010 ; 131 : 1-9.   DOI
7 Que F, Mao L, Pan X. Antioxidant activities of five Chinese rice wines and the involvement of phenolic compounds. Food Res Int. 2006 ; 39(5) : 581-587.   DOI
8 Canadanovic-Brunet JM, Djilas SM, Cetkovic GS, Tumbas VT. Free-radical scavenging activity of wormwood (Artemisia absinthium L.) extracts. J. Sci. Food Agr. 2005 ; 85 : 265-272.   DOI
9 Lee JH, Park AR, Choi DW, Kim JD, Kim JC, Ahn JH, Lee HY, Choe M, Choi KP, Shin IC, Park HJ. Analysis of chemical compositions and electrondonating ability of 4 Korean wild sannamuls. Korean J. Medicinal Crop. 2011 ; 19 : 111-116.   DOI
10 Lee SO, Lee HJ, Yu MH, Im HG, Lee IS. Total polyphenol contents and antioxidant activities of methanol extracts from vegetables produced in Ullung island. Korean Journal of Food Science and Technology. 2005 ; 37 : 233-240.
11 Smith JR, Pereira-Smith OM. Replicative senescence: implications for in vivo aging and tumor suppression. Science. 1996; 5 : 63-67.
12 Allsopp R C, Vaziri H, Patterson C, Goldstein S, Younglai EV. Telomere length predicts replicative capacity of human fibroblasts. Proc. Natl. Acad. Sci. USA. 1992 ; 89 : 10114-10118.   DOI
13 Takahashi H, Kosaka M, Watanabe Y, Nakade K, Fukuyama Y. Synthesis and Neuroprotective activity of bergenin derivatives with antioxidant activity. Bioorganic & Medicinal Chemistry. 2003 ; 11 : 1781-1788.   DOI
14 Mitscher LA, Park YH, Clark D, Beal JL. Antimicrobial agents from higher plants, antimicrobial isoflavonoids and related substances from Glycyrrhiza glabra L. var. typica. J Nat Prod. 1980 ; 43 : 259-327.   DOI
15 Jeong SJ, Lee JH, Song NH, Seong SN, Lee SE, Baeg NI. Natural products, organic chemistry: Screening for antioxidant activity of plant medicinal extracts. J Kor Soc App Biol Chem. 2004 ; 27 : 135-140.
16 Chan KM, Decker EA, Means WJ. Extraction and activity of camosine a naturally occurring antioxidant in beef muscle. J Food Sci. 1993 ; 58 : 1-4.   DOI
17 Kim SS, Kim JE, Hyun CG, Lee NH. Neolitsea aciculata essential oil inhibits drug-resistant skin pathogen growth and Propionibacterium acnesinduced inflammatory effects of human monocyte leukemia. Natural Product Communications. 2011 ; 6 : 1193-1198.
18 Cui JZ. A Study on population structure of plantago asiatica L. in East Asia. Pusan National University Master's degree. 2002.
19 Lin KH, Yang YY, Yang CM, Huang MY, Lo HF, Liu KC, Lin HS, Chao PY. Antioxidant activity of herbaceous plant extracts protect against hydrogen peroxide-induced DNA damage in human lymphocytes. BMC Res Notes. 2013 ; 26(6) : 490.
20 Park BG, Lee HS, Jung SH, Koo YC, Hong CO, Lee SJ, Lee KW. Single & 14-day repeated oral toxicity study and genotoxicological safety estimate of Plantamajoside isolated from Plantago asiatica. J. Toxicol. Pub. Health. 2007 ; 23(1) : 79-86.
21 Masaki H, Sakaki S, Atsumi T, Sakurai H. Aactiveoxygen sacvenging activity of plants extracts. Biol Pharm Bull. 1995 ; 18(1) : 162-166.   DOI
22 Velioglu YS, Mazza G, Gao L, Oomah BD. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J Agric Food Chem. 1998 ; 46 (10) : 4113-4117.   DOI
23 Jia Z, Tang M, Wu J. The determination of flavonoid contents in mulberry and they scavenging effects on super-oxide radicals. Food Chem. 1999 ; 64: 555-559.   DOI
24 Kim YJ, Park HJ, Lee JS, Do EJ, Sohn HR, Jeon SM, Yeum JH, Kim MR. Antioxidant effect of ethanol extract from Poria cocos depending on cultivation methods. Kor. J. Herbol. 2016 ; 31(5) : 107-114.   DOI
25 Kim SY, Park HJ, Kim MR. Free radical scavenging effect of water extract from Bansi (Diospyroskaki), Cheongdo flat persimmon. J.A.O.M. 2016 ; 16(1) : 69-78.
26 Blois MS. Antioxidant determination by the use of stable free radical. Nature. 1958 ; 191 : 2299-2200.   DOI
27 Ito N, Fukushima S, Hasebawa A. Carcinogenicity of BHA in F344 rats. J Natl Cancer Inst. 1983 ; 70 : 343-352.
28 Van den Berg R, Haenen GR, Van den Berg H, Bast A. Applicability of an improved trolox epuivalent anti-oxidant capacity (TEAC) assay for evaluation of antioxidant capacity measurements of mixtures. Food Chem. 1999 ; 66 : 511-517.   DOI
29 Albertazzi P, Steel SA, Clifford E, Bottazzi M. Attiudes towards and use of dietary supplementation in a sample of postmenopausal women. Climacteric. 2002 ; 5 : 374-382.   DOI
30 Brannen AL. Toxicology and biochemistry of butylated hydroxy toluene and butylated hydroxy anisole. J Am Oil Chem Soc. 1975 ; 52 : 59-63.   DOI
31 Murai M, Tamayama Y, Nishibe S. Phenylethanoids in the herb of Plantago lanceolata and inhibitory effect on arachidonic acid-induced mouse ear edema1. Planta medica. 1995 ; 61(05) : 479-480.   DOI
32 Aritomi M. Homoplantaginin, a new flavonoid glycoside in leaves of Plantago asiatica Linnaeus. Chemical and Pharmaceutical Bulletin. 1967 ; 15(4) : 432-434.   DOI
33 Cha NH, Seo EJ, Sok SR. Factors influencing the successful aging of older korean adults. Contemporary Nurse. 2012 ; 41 : 78-87.   DOI
34 Albertazzi P, Steel SA, Clifford E, Bottazzi M. Attiudes towards and use of dietary supplementation in a sample of postmenopausal women. Climacteric. 2002 ; 5 : 374-382.   DOI
35 Harold ES, Darrell EA, Evan IF, John AM. A review of the interaction among dietary antioxidants and reactive oxygen species. J Nut Biochem. 2007 ; 18 : 567-579.   DOI
36 Smith JR, Pereira-Smith OM. Replicative senescence: implications for in vivo aging and tumor suppression. Science. 1996 ; 5 : 63-67.
37 Maxwell SJ. Prospects for the use of antioxidant therapies. Drugs. 1995 ; 49 : 345-361.   DOI
38 Allsopp RC, Vaziri H, Patterson C, Goldstein S, Younglai EV. Telomere length predicts replicative capacity of human fibroblasts. Proc. Natl. Acad. Sci. USA. 1992 ; 89 : 10114-10118.   DOI
39 Chung HY, Sung B, Jung KJ, Zou Y, Yu BP. The molecular inflammatory process in aging. Antioxid. Redox Sign. 2006 ; 8 : 572-581.   DOI
40 Lee SH, Hong LJ, Park HG, Ju SS, Kim GT. Functional characteristics from the barley leaves and its antioxidant mixture. J. Korean Soc. Agric. Chem. Biotechnol. 2003 ; 46 : 333-337.
41 Marnett L. Oxiradicals and DNA damage. Carcinogenesis. 2000 ; 21 : 361-370.   DOI
42 Oh SJ. Aging of human body. Seoul : Tamkudang. 2005 : 204-205.
43 Sung HK, Park MH, Jang KJ. Medicinal plants native to wild. 2nd de., Munyei press. Seoul, Korea. 2008 ; 86.
44 Yang Q, Qi M, Tong R, Wang D, Ding L, Li Z, Yang L. Plantago asiatica L. seed extract improves lipid accumulation and hyperglycemia in high-fat dietinduced obese mice. Int. J. Mol. Sci. 2017 ; 18(7) : 1393.   DOI
45 Turel I, Ozbek H, Erten R, Oner AC, Cengiz N, Yilmaz O. Hepatoprotective and anti-inflammatory activities of Plantago major L. Indian J. Pharmacol. 2009 ; 41(3) : 120.   DOI
46 Kim SY, Jeong MJ, Kim YJ, Lee UT, Choo ST, Kim HH, Kim MR. Effect of Plantaginis asiaticae Folium water extract on body fat loss in high fat-induced obese C57BL/6 mice. Kor. J. Herbology. 2018 ; 33(2) : 59-68.
47 Komoda Y, Chujo H, Ishihara S, Uchida M. HPLC quantitative analysis of plantaginin in Shazenso (Plantago asiatica L.) extracts and isolation of plantamajoside. Iyo Kizai Kenkyujo hokoku. Reports of the institute for medical and dental engineering, Tokyo medical and dental university. 1989 ; 23 : 81-85.
48 Yin JY, Nie SP, Zhou C, Wan Y, Xie MY. Chemical characteristics and antioxidant activities of polysaccharide purified from the seeds of Plantago asiatica L. J. Sci. Food Agric. 2010 ; 90(2) : 210-217.   DOI
49 Choi SY, Jung SH, Lee HS, Park KW, Yun BS, Lee KW. Glycation inhibitory activity and the identification of an active compound in Plantago asiatica extract. Phytother. Res. 2008 ; 22(3) : 323-329.   DOI
50 Nishibe S. The plant origins of herbal medicines and their quality evaluation. J. Pharm. Soc. Jpn. 2002 ; 122(6) : 363-380.   DOI
51 Kim HK, Kwon YJ, Kim KH, Jeong YH. Changes of total polyphenol content and electron donating ability of Aster glehni extracts with different microwave-assisted extraction conditions. Korean J. Food Sci. Technol. 2000 ; 32 : 1022-1028.
52 Meller NJ, Rice-Evans C, Davies MJ, Gopinathan V, Milner A. A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clin Sci. 1993 ; 84 : 407-412.   DOI
53 Diplock AT. Will the good fairies please prove to us that vitamin E lessens human degenerative disease? Free Rad. 1997 ; 27 : 511-532.