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Effects of flaC Mutation on Stringent Response-Mediated Bacterial Growth, Toxin Production, and Motility in Vibrio cholerae

  • Kim, Hwa Young (Department of Microbiology and Immunology, Brain Korea PLUS Project for Medical Science, Institute for Immunology and Immunological Diseases, Yonsei University College of Medicine) ;
  • Yu, Sang-Mi (Freshwater Bioresources Utilization Division, Nakdonggang National Institute of Biological Resources) ;
  • Jeong, Sang Chul (Freshwater Bioresources Utilization Division, Nakdonggang National Institute of Biological Resources) ;
  • Yoon, Sang Sun (Department of Microbiology and Immunology, Brain Korea PLUS Project for Medical Science, Institute for Immunology and Immunological Diseases, Yonsei University College of Medicine) ;
  • Oh, Young Taek (Freshwater Bioresources Utilization Division, Nakdonggang National Institute of Biological Resources)
  • Received : 2017.12.18
  • Accepted : 2018.02.26
  • Published : 2018.05.28

Abstract

The stringent response (SR), which is activated by accumulation of (p)ppGpp under conditions of growth-inhibiting stresses, plays an important role on growth and virulence in Vibrio cholerae. Herein, we carried out a genome-wide screen using transposon random mutagenesis to identify genes controlled by SR in a (p)ppGpp-overproducing mutant strain. One of the identified SR target genes was flaC encoding flagellin. Genetic studies using flaC and SR mutants demonstrated that FlaC was involved in bacterial growth, toxin production, and normal flagellum function under conditions of high (p)ppGpp levels, suggesting FlaC plays an important role in SR-induced pathogenicity in V. cholerae.

Keywords

References

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