Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Administration of Alphas1-Casein Hydrolysate Increases Sleep and Modulates GABAA Receptor Subunit Expression

  • Yayeh, Taddesse (Department of Molecular Medicine and TIDRC, School of Medicine, Ewha Womans University) ;
  • Leem, Yea-Hyun (Department of Molecular Medicine and TIDRC, School of Medicine, Ewha Womans University) ;
  • Kim, Kyung-Mi (Life Science Research Institute, Novarex Co., Ltd) ;
  • Jung, Jae-Chul (Life Science Research Institute, Novarex Co., Ltd) ;
  • Schwarz, Jessica (Ingredia SA) ;
  • Oh, Ki-Wan (Department of Pharmacy, College of Pharmacy, Chungbuk National University) ;
  • Oh, Seikwan (Department of Molecular Medicine and TIDRC, School of Medicine, Ewha Womans University)
  • Received : 2017.04.06
  • Accepted : 2017.09.19
  • Published : 2018.05.01
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Abstract

Sleep is the most basic and essential physiological requirement for mental health, and sleep disorders pose potential risks of metabolic and neurodegenerative diseases. Tryptic hydrolysate of ${\alpha}_{S1}$-casein (${\alpha}_{S1}-CH$) has been shown to possess stress relieving and sleep promoting effects. However, the differential effects of ${\alpha}_{S1}-CH$ on electroencephalographic wave patterns and its effects on the protein levels of ${\gamma}$-aminobutyric acid A ($GABA_A$) receptor subtypes in hypothalamic neurons are not well understood. We found ${\alpha}_{S1}-CH$ (120, 240 mg/kg) increased sleep duration in mice and reduced sleep-wake cycle numbers in rats. While ${\alpha}_{S1}-CH$ (300 mg/kg) increased total sleeping time in rats, it significantly decreased wakefulness. In addition, electroencephalographic theta (${\theta}$) power densities were increased whereas alpha (${\alpha}$) power densities were decreased by ${\alpha}_{S1}-CH$ (300 mg/kg) during sleep-wake cycles. Furthermore, protein expressions of $GABA_A$ receptor ${\beta}_1$ subtypes were elevated in rat hypothalamus by ${\alpha}_{S1}-CH$. These results suggest ${\alpha}_{S1}-CH$, through $GABA_A$ receptor modulation, might be useful for treating sleep disorders.

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References

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