Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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Workflow of Cryo-Electron Microscopy and Status of Domestic Infrastructure

  • Choi, Ki Ju (Division of Materials and Structural Analysis, Thermo Fisher Scientific Inc.) ;
  • Shin, Jae In (Division of Materials and Structural Analysis, Thermo Fisher Scientific Inc.) ;
  • Lee, Sung Hun (Division of Materials and Structural Analysis, Thermo Fisher Scientific Inc.)
  • Received : 2018.03.02
  • Accepted : 2018.03.26
  • Published : 2018.03.30
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Abstract

Cryo-electron microscopy (cryo-EM) allows the analysis of the near-native structures of samples such as proteins, viruses, and sub-cellular organelles at the sub-nano scale. With the recent development of analytical methods, this technique has achieved remarkable results. The importance of cryo-EM gained wide recognition due to last year's award of the Nobel Prize in Chemistry. To help promote the knowledge of this technique, this paper introduces the basic workflows of cryo-EM and domestic cryo-EM service institutes.

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References

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