Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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Image Processing and Cryo-Transmission Electron Microscopy; Example of Human Proteasome

  • Choi, Hyosun (BK21 Plus Program, Department of Senior Healthcare, Graduate School, Eulji University) ;
  • Jeon, Hyunbum (Nanobioimaging Center, National Instrumentation Center for Environmental Management, Seoul National University) ;
  • Noh, Seulgi (BK21 Plus Program, Department of Senior Healthcare, Graduate School, Eulji University) ;
  • Kwon, Ohkyung (Nanobioimaging Center, National Instrumentation Center for Environmental Management, Seoul National University) ;
  • Mun, Ji Young (Department of Structure and Function of Neural Network, Korea Brain Research Institute)
  • Received : 2018.03.12
  • Accepted : 2018.03.27
  • Published : 2018.03.30
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Abstract

Cryo-transmission electron microscopy (cryo-TEM) allows us to perform structural analysis of a analyses of large protein complexes, which are difficult to analyze using X-ray crystallography or nuclear magnetic resonance. The most common examples of proteins used are ribosomes and proteasomes. In this paper, we briefly describe the advantage of cryo-TEM and the process of two-dimensional classification by considering a human proteasome as an example.

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References

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