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http://dx.doi.org/10.9729/AM.2018.48.1.6

Workflow of Cryo-Electron Microscopy and Status of Domestic Infrastructure  

Choi, Ki Ju (Division of Materials and Structural Analysis, Thermo Fisher Scientific Inc.)
Shin, Jae In (Division of Materials and Structural Analysis, Thermo Fisher Scientific Inc.)
Lee, Sung Hun (Division of Materials and Structural Analysis, Thermo Fisher Scientific Inc.)
Publication Information
Applied Microscopy / v.48, no.1, 2018 , pp. 6-10 More about this Journal
Abstract
Cryo-electron microscopy (cryo-EM) allows the analysis of the near-native structures of samples such as proteins, viruses, and sub-cellular organelles at the sub-nano scale. With the recent development of analytical methods, this technique has achieved remarkable results. The importance of cryo-EM gained wide recognition due to last year's award of the Nobel Prize in Chemistry. To help promote the knowledge of this technique, this paper introduces the basic workflows of cryo-EM and domestic cryo-EM service institutes.
Keywords
Cryo-electron microscopy; Workflow; Single particle analysis; Cryo-electron tomography;
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