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http://dx.doi.org/10.9729/AM.2018.48.1.1

Image Processing and Cryo-Transmission Electron Microscopy; Example of Human Proteasome  

Choi, Hyosun (BK21 Plus Program, Department of Senior Healthcare, Graduate School, Eulji University)
Jeon, Hyunbum (Nanobioimaging Center, National Instrumentation Center for Environmental Management, Seoul National University)
Noh, Seulgi (BK21 Plus Program, Department of Senior Healthcare, Graduate School, Eulji University)
Kwon, Ohkyung (Nanobioimaging Center, National Instrumentation Center for Environmental Management, Seoul National University)
Mun, Ji Young (Department of Structure and Function of Neural Network, Korea Brain Research Institute)
Publication Information
Applied Microscopy / v.48, no.1, 2018 , pp. 1-5 More about this Journal
Abstract
Cryo-transmission electron microscopy (cryo-TEM) allows us to perform structural analysis of a analyses of large protein complexes, which are difficult to analyze using X-ray crystallography or nuclear magnetic resonance. The most common examples of proteins used are ribosomes and proteasomes. In this paper, we briefly describe the advantage of cryo-TEM and the process of two-dimensional classification by considering a human proteasome as an example.
Keywords
Cryo-transmission electron microscopy; Proteasome; Two-dimensional classification; Negative staining;
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