The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Isolation and identification of Aureobasidium spp. from flowers of the Jeolla-do province in Korea

호남 지역 꽃으로부터 야생효모 Aureobasidium속 분리 및 동정

  • Kim, Jeong-Seon (Agricultural Microbiology Division, National Institute of Agricultural Science) ;
  • Lee, Miran (Agricultural Microbiology Division, National Institute of Agricultural Science) ;
  • Song, Mi Young (Agricultural Microbiology Division, National Institute of Agricultural Science) ;
  • Kwon, Soon-Wo (Agricultural Microbiology Division, National Institute of Agricultural Science) ;
  • Kim, Soo-Jin (Agricultural Microbiology Division, National Institute of Agricultural Science) ;
  • Hong, Seung-Beom (Agricultural Microbiology Division, National Institute of Agricultural Science) ;
  • Park, Byeong-Yong (Agricultural Microbiology Division, National Institute of Agricultural Science) ;
  • Yun, Bong Sik (Division of Biotechnology and Advanced institute of Environmental and Bioscience, Chonbuk National University)
  • 김정선 (국립농업과학원 농업미생물과) ;
  • 이미란 (국립농업과학원 농업미생물과) ;
  • 송미영 (국립농업과학원 농업미생물과) ;
  • 권순우 (국립농업과학원 농업미생물과) ;
  • 김수진 (국립농업과학원 농업미생물과) ;
  • 홍승범 (국립농업과학원 농업미생물과) ;
  • 박병용 (국립농업과학원 농업미생물과) ;
  • 윤봉식 (전북대학교 환경생명자원대학 생명공학부)
  • Received : 2018.11.09
  • Accepted : 2018.11.21
  • Published : 2018.12.01
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Abstract

To study the characteristics of yeasts, 433 strains of the genus Aureobasidium were isolated from the flowers collected from Jeolla-do in Korea, and the diversity of the strains was confirmed through molecular phylogenetic and morphological analyses. Based on phylogenetic analysis of LSU rDNA seguences, the Aureobasidium strains from the Jeolla-do province were classified into six groups. The dominant species of flower-derived yeasts were Groups A and D. Since Groups B, E, and F were found only in Jeollanam-do, we can infer that the Aureobasidium is distributed more widely in Jeollanam-do than in the Jeollabuk-do province. Through LSU and ITS rDNA sequence analyses, Group A was identified as A. pullulans, Group B as A. melanogenum, and Group F as a putative new species of Aureobasidium. Groups C, D, and E do not completely match with A. leucospermi, A. namibiae, or A. subglaciale by LSU or ITS rDNA analysis but are closely related to those species. Comparisons of colony morphology are likely to be more helpful in distinguishing Groups C and D. The results of this study can provide useful characteristics for future studies of the genus Aureobasidium.

다양한 Aureobaisidum속을 발굴하여 흑효모의 유용한 특성을 활용하기 위해 전라도 지역 꽃에서 효모 433균주를 분리하고 분자계통학 및 형태학적 분석을 통해 다양성을 확인하였다. large subunit (LSU) rDNA 염기서열 분석을 기준으로 전라도 지역의 Aureobasidium속을 6그룹으로 분류한 후, LSU와 ITS rDNA 염기서열 분석을 통해 전라도 지역 꽃에서 유래한 효모의 주요 우점종이 Group A와 Group D임을 확인할 수 있었다. GroupB, E, F는 전라남도에만 분포하는 것으로 보아 전라북도에 비해 전라남도에서 다양한 Aureobasidium종이 분포하는 것으로 생각된다. Group A는 A. pullulans, Group B는 A. melanogenum, Group F는 Aureobasidium 신종 후보군으로 분류할 수 있었다. Group C, D, E는 LSU와 ITS rDNA 분석에서 A. leucospermi, A. namibiae, A. subglaciale 사이의 명확하게 분류되지 않았으나 콜로니 형태에서 구분 가능한 특성을 보인 것으로 보아, Aureobasidium은 분자계 통학적 분석방법과 형태적 동정을 병행하여 종 수준 동정을 보완할 수 있을 것으로 생각된다.

Keywords

GNHHDL_2018_v46n4_415_f0001.png 이미지

Fig. 1. Phylogenetic tree of Aureobasidium spp. based on the nucleotide sequences of large subunit (LSU) rDNA domain D1/D2 (A), internal transcribed spacers (ITS) rDNA (B), ITS and LSU rDNA (C). The trees was generated by the maximum likelihood method, using MEGA7.0. T, Type strains.

GNHHDL_2018_v46n4_415_f0002.png 이미지

Fig. 1. Phylogenetic tree of Aureobasidium spp. based on the nucleotide sequences of large subunit (LSU) rDNA domain D1/D2 (A), internal transcribed spacers (ITS) rDNA (B), ITS and LSU rDNA (C). The trees was generated by the maximum likelihood method, using MEGA7.0. T, Type strains.

GNHHDL_2018_v46n4_415_f0003.png 이미지

Fig. 2. Colony morphology of Aureobasidium isolates for incubated 7~14 day on potato dextrose agar and malt extract agar.

Table 1. Yeasts groups and strains derived from fowers in Jeolla province in Korea

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Table 1. Yeasts groups and strains derived from fowers in Jeolla province in Korea

GNHHDL_2018_v46n4_415_t0002.png 이미지

Table 2. Homology matrix between Aureobasidium spp. of fowers in Jeolla province in Korea and type strains, generated by DNAMAN software 7.0

GNHHDL_2018_v46n4_415_t0003.png 이미지

Table 2. Homology matrix between Aureobasidium spp. of fowers in Jeolla province in Korea and type strains, generated by DNAMAN software 7.0

GNHHDL_2018_v46n4_415_t0004.png 이미지

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