The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Aspergillus caninus (Syn: Phialosimplex caninus): a New Isolate from Field Soils in Korea

  • Adhikari, Mahesh (Division of Biological Resources Sciences, Kangwon National University) ;
  • Gurung, Sun Kumar (Division of Biological Resources Sciences, Kangwon National University) ;
  • Kim, Sang Woo (Division of Biological Resources Sciences, Kangwon National University) ;
  • Lee, Hyun Goo (Division of Biological Resources Sciences, Kangwon National University) ;
  • Ju, Han Jun (Division of Biological Resources Sciences, Kangwon National University) ;
  • Gwon, Byeong Heon (Division of Biological Resources Sciences, Kangwon National University) ;
  • Kosol, San (Division of Biological Resources Sciences, Kangwon National University) ;
  • Bazie, Setu (Division of Biological Resources Sciences, Kangwon National University) ;
  • Lee, Hyang Burm (Division of Food Technology, Biotechnology and Agrochemistry, Chonnam National University) ;
  • Lee, Youn Su (Division of Biological Resources Sciences, Kangwon National University)
  • Received : 2018.07.13
  • Accepted : 2018.08.23
  • Published : 2018.12.01
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Abstract

During the study of indigenous fungal communities in soil samples collected from various field soils in Sancheong, Gyeongsangnam-do, Korea in 2017, several species of Aspergillus were discovered. Aspergillus caninus (KNU17-7) was isolated, identified, and described based on the results from macro and micro morphological characteristics and molecular characterization. Morphologically, it was identified using five different growth media: potato dextrose agar, oatmeal agar, yeast extract sucrose agar, czapek yeast extract agar, and malt extract agar. For the molecular identification, sequencing of internal transcribed spacer, ${\beta}-tubulin$, and calmodulin genes was performed. Based on this characterization, our study isolate was identified as Aspergillus caninus. This fungal species has not been officially reported in Korea before, and we report here with its morphological and molecular phylogenetic characterization.

Keywords

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Fig. 1. Morphological features of the Aspergillus caninus KNU17-7 colony grown for 7 days on potato dextrose agar (PDA), oatmeal agar (OMA), yeast extract sucrosea gar (YESA), malt extract agar (MEA), and Czapek yeast extract agar (CYEA) at 25°C. A, F, front and back side of the colony on PDA; B, G, front and back side on OMA; C, H, front and back side oYn ESA; D, I, front and back side on MEA; E, J, front and back side on CYEA.

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Fig. 2. Microstructures of the isolate Aspergillus caninus KNU17-7. Front colony from left to right. A, B, microscopy of conidiophores with phialides; C, microscopy of conidia; D, E, scanning electron microscopy of the conidia; F~H, scanning electron microscopy o fconidiophores-bearing conidia.

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Fig. 3. Neighbor-joining phylogenetic analysis of the partial 18S-ITS1-5.8S-ITS2-28S rDNA sequences of Aspergillus caninus KNU17-7. Tree was constructed using the MEGA ver. 6 program. Sequences obtained in the study are shown in boldface. The mark (T) indicates the type strain. Numerical values (> 50) on branches are the bootstrap values as percentage of bootstrap replication from a 1,000 replicate analysis. Scale bar represents the number of substitutions pers ite.

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Fig. 4. Neighbor-joining phylogenetic analysis of β-tubulin (tub2/BenA) gene sequences of Aspergillus caninus KNU17-7. A phylogenetic tree was constructed using MEGA ver. 6. Sequences obtained in the study are shown in boldface. Numerical values (> 50) on branches are the bootstrap values as percentage of bootstrap replication from a 1,000 replicate analysis. Scale bar represents the number of substitutions per site.

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Fig. 5. Neighbor-joining phylogenetic analysis of Calmodulin (CaM) gene sequences of Aspergillus caninus KNU17-7. Tree was constructed using MEGA ver. 6. Sequences obtained in the study are shown in boldface. Numerical values (> 50) on branches are the bootstrap values as percentage of bootstrap replication from a 1,000 replicate analysis. Scale bar represents the number of substitutions per site.

Table 1. Primers used for amplifcation and sequencing.

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Table 2. Thermal cycle programs used for amplifcation.

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Table 3. Morphological comparison of the study isolate KNU17-7 with the previously reported Aspergillus caninus.

GNHHDL_2018_v46n4_383_t0003.png 이미지

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