한국균학회지 (The Korean Journal of Mycology)

  • 제46권4호
  • /
  • Pages.375-382
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  • 2018
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  • 0253-651X(pISSN)
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  • 2383-5249(eISSN)
한국균학회 (The Korean Society of Mycology)
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Molecular Phylogeny and Morphology Reveal the Underestimated Diversity of Mortierella (Mortierellales) in Korea

  • Lee, Jae-Sung (Department of Biology, Kunsan National University) ;
  • Nam, Bora (Department of Biology, Kunsan National University) ;
  • Lee, Hyang Burm (Division of Food Technology, Biotechnology and Agrochemistry, Chonnam National University) ;
  • Choi, Young-Joon (Department of Biology, Kunsan National University)
  • 투고 : 2018.10.22
  • 심사 : 2018.11.14
  • 발행 : 2018.12.01
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초록

Members of the genus Mortierella (Mortierellales) are filamentous fungi, which are found on nearly all substrates, but more frequently in soil. Till date, 7 species of Mortierella have been reported in Korea, but being a ubiquitous group with high species diversity in temperate zones, this number is still low. During a survey of fungal biodiversity in Korea, we collected many isolates of Mortierella, and through morphological and molecular phylogenetic analyses, identified them to be 3 previously unrecorded species, namely, M. chienii, M. epicladia, and M. gamsii. A total of 10 Mortierella species in Korea, including the 3 species reported in the present study, are widely distributed in 5 out of 7 phylogenetic groups of this genus. This indicates that the diversity of Mortierella was so far underestimated in Korea. Multi-locus sequence analysis is required to provide a more reliable backbone for some uncertain phylogenetic groupings and to more clearly define a species of Mortierella, which would encourage deeper research in the diversity and ecological roles of Mortierella and allied genera.

키워드

GNHHDL_2018_v46n4_375_f0001.png 이미지

Fig. 1. Minimum evolution tree based on the internal transcribed spacer rDNA sequences. Bootstrapping support values of minimum evolution and maximum likelihood methods higher than 60% are given above or below the branches. The species revealed in the present study are shown in blue while the previously known species are shown in red. The scale bar equals the number of nucleotide substitutions per site.

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Fig. 2. Cultural and morphological features of Mortierella chienii NIBRFG0000501464 (A~I), Mortierella epicladia NIBRFG0000502787 (J~R), and Mortierella gamsii NIBRFG0000501465 (S~W). Colonies on potato dextrose agar at top (A, J) and at bottom view (B, K). Colonies on V8 agar at top (C, L, S) and at bottom view (D, M, T), Colonies on corn meal agar at top (E, N, U) and at bottom view (F, O, V). P, Sporangiophore; G~I, Q, W, Sporangium; R, Sporangiospore (scale bars: G = 50 μm, P = 20 μm, H~I, G~R, W = 10 μm).

Table 1. Information of Mortierella strains isolated from riverside soils in Korea

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