The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Undescribed Fungal Species of Eupenicillium, Mortierella, and Trichoderma Isolated in the Vicinity of Demilitarized Zone in Yeoncheon-gun, Gyeonggi-do, Korea

경기도 연천비무장지대 근역에서 분리한 국내 미기록 Eupenicillium, Mortierella, Trichoderma 진균 종 보고

  • 안금란 (단국대학교 미생물학과) ;
  • 김지은 (단국대학교 미생물학과) ;
  • 오윤석 (단국대학교 미생물학과) ;
  • 이경민 (단국대학교 미생물학과) ;
  • 진협 (단국대학교 미생물학과) ;
  • 김민욱 (단국대학교 미생물학과) ;
  • 김준영 (단국대학교 미생물학과) ;
  • 김성환 (단국대학교 미생물학과)
  • Received : 2018.08.01
  • Accepted : 2018.09.06
  • Published : 2018.12.01
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Abstract

As an effort to explore fungal diversity, fungal survey was undertaken in 2017 in the vicinity of demilitarized zone (DMZ) located in Yeoncheon-gun, Gyeonggi-do, Korea. For the survey, wild plants and soils were sampled and subjected to fungal isolation. A total of 18 genera and 23 species including five unrecorded fungal species, Mortierella sclerotiella, M. sossauensis, M. verticillate, Eupenicillium saturniforme, and Trichoderma hispanicum, were obtained from the survey. This study described their morphological characteristics including colony features formed on media, light microscopic images and molecular characteristics of nucleotide sequences of the internal transcribed spacer (ITS) region, 18S and 28S rDNA, ${\beta}-tubulin$ gene, calmodulin, and translation elongation factor $1{\alpha}$ ($tef1{\alpha}$) nDNA genes.

비무장지대 일원의 진균 다양성을 연구하기 위한 일환으로 경기도 연천군 마전리에 있는 산과 황진리에 있는 임진강변에서 11가지 식물체 시료와 토양시료 2개를 채집하여 진균을 분리 동정하였다. 그 결과 총 18속 23종의 진균을 확인하였다. 이 중 Eupenicilliumsaturniforme, Mortierellasclerotiella, M. sossauensis, M. verticillate, Trichoderma hispanicum등 5종의 진균이 국내에 보고가 되어있지 않는 미기록 종이었다. 본 연구에서 이들의 형태적, 분자적 특성을 기술하였다.

Keywords

GNHHDL_2018_v46n4_359_f0001.png 이미지

Fig 1. Photos of soil and plant sources sampled in this study for the isolation of fungi in the vicinity of DMZ in Yeoncheon-gun. A row, Sampled in Majeon-ri; B row, Sampled in Hwangjin-ri; A1, Persicaria hydropiper; A2, Rhus javanica; A3, Amorpha fruticose; A4, Osmunda japonica; A5, Corylus heterophylla var. thunbergii; A6, Soil; B1, Populus davidiana Dode; B2, Prunus persica; B3, Populus tomentiglandulosa; B4, Phragmites japonica; B5, Betula dahurica; B6, Digitaria ciliairs; B7, Soil; Red arrow, soil sampling site and disease symptom like part in the sampled plants.

GNHHDL_2018_v46n4_359_f0002.png 이미지

Fig 2. Colony morphology of the fungal species isolated in this study. Cultures were grown on potato dextrose agar at 25℃ for 7 days. 1, Alternaria alternata; 2, Aspergillus fumigatus; 3,Cladosporim oxysporum; 4, Coprinellus radians; 5, Curvularia buchloes; 6, Daldinia childiae; 7, Daldinia pyrenaica; 8, Discosia pseudoartocreas; 9, Epicoccum nigrum; 10, Eupenicillium saturniforme; 11, Gibberella zeae; 12, Leptosphaeria spegazzinii; 13, Magnaporthe oryzae; 14, Mortierella sclerotiella; 15, Mortierella sossauensis; 16, Mortierella verticillata; 17, Mucor racemosus; 18, Nigrospora oryzae; 19, Penicillium verruculosum; 20, Stagonosporopsis cucurbitacearum; 21, Trichoderma atroviride; 22, Strichoderma hamatum; 23, Trichoderma hispanicum.

GNHHDL_2018_v46n4_359_f0003.png 이미지

Fig 3. Light microscope morphology of the five unrecorded fungal speceis isolated from various sources inY eoncheon-gun. A, Mortierella sclerotiella; B, Eupenicillium saturniforme; C, Mortierella sossauensis ; D, Mortierella verticillate ; E, Trichoderma hispanicum (scale bars = 10 μm).

Table 1. List of the fungal species isolated and identifed in this study from plants and soils in the vicinity of DMZ in Yeoncheon-gun.

GNHHDL_2018_v46n4_359_t0001.png 이미지

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