The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Influence of High Hydrostatic Pressure Treatment after Germination on Anti-proliferation Effects of Soyasaponin-rich Fraction in Black Soybean (Glycine max L.)

발아와 고압처리가 검정콩 사포닌 추출물의 암세포주 증식억제에 미치는 영향

  • Kim, Min Young (Dept. of Food Science and Biotechnology, Chungbuk National University) ;
  • Lee, Yoon Jeong (Dept. of Food Science and Biotechnology, Chungbuk National University) ;
  • Song, Myeong Seob (Dept. of Food Science and Biotechnology, Chungbuk National University) ;
  • Oh, Hyunah (Dept. of Food Science and Biotechnology, Chungbuk National University) ;
  • Kim, Kyung Mi (National Academy of Agricultural Science, Rural Development Administration) ;
  • Kang, Tae Su (Dept. of Food Science and Biotechnology, Chungbuk Provincial University of Science and Technology) ;
  • Lee, Youn Ri (Dept. of Food and Nutrition, Daejeon Health Sciences College) ;
  • Lee, Junsoo (Dept. of Food Science and Biotechnology, Chungbuk National University) ;
  • Jeong, Heon Sang (Dept. of Food Science and Biotechnology, Chungbuk National University)
  • 김민영 (충북대학교 식품생명공학과) ;
  • 이윤정 (충북대학교 식품생명공학과) ;
  • 송명섭 (충북대학교 식품생명공학과 대학원) ;
  • 오현아 (충북대학교 식품생명공학과 대학원) ;
  • 김경미 (농촌진흥청 국립농업과학원) ;
  • 강태수 (충북도립대학교 바이오식품과학과) ;
  • 이연리 (대전보건대학교 식품영양학과) ;
  • 이준수 (충북대학교 식품생명공학과) ;
  • 정헌상 (충북대학교 식품생명공학과)
  • Received : 2018.10.11
  • Accepted : 2018.11.30
  • Published : 2018.12.31
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Abstract

The objective of this study was to determine the effect of high hydrostatic pressure (HHP) treatment on proliferation of human cancer cell lines (MCF-7, HCT-116, PC-3 and AGS) of crude soyasaponin extracts in germinated black soybean. Black soybean was germinated and subjected to HHP, followed by preparation of crude soyasaponin extracts. Cell treatments done with extracts less than $400{\mu}g/mL$ concentrations had no significant effect on 3T3-L1 adipocyte cell viability. The inhibitory effect of crude soyasaponin extracts with germination periods and applied pressure on breast cancer cell (MCF-7), human colon cancer cell (HCT-116), human gastriccancer cell (AGS) and prostate cancer cell (PC-3) growth were investigated using MTT assay. The highest anti-proliferation of human cancer cell line of crude soyasaponin extracts was observed at 150 MPa treatment after germination for 4 days (150 MPa-Day 4). The cell viability on MCF-7, HCT-116, PC-3 and AGS cell lines of crude soyasaponin extract in 150 MPa-Day4 was 48.82%, 57.37%, 39.89% and 23.94% at $400{\mu}g/mL$, respectively. These results suggest that soyasaponin extracts from black soybean subjected to HHP after germination may mediate physiological activity.

Keywords

Table 1. Changes in crude soyasaponin contents and cell viability for raw 264.7 and 3T3-L1 cell of crude saponin extracts with germination and high hydrostatic pressure treatment (50, 100 and 150 MPa for 24 h) in black soybean

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Table 2. Anti-proliferative effects of crude saponin extracts of black soybean treated by different high hydrostatic pressure treatments (0.1~150 MPa) and germination (0~4 days) in MCF-7 breast cancer cell

HGSPB1_2018_v31n6_836_t0002.png 이미지

Table 3. Anti-proliferative effects of crude saponin extracts of black soybean treated by different high hydrostatic pressure treatments (0.1~150 MPa) and germination (0~4 days) in HCT-116 colon cancer cell

HGSPB1_2018_v31n6_836_t0003.png 이미지

Table 4. Anti-proliferative effects of crude saponin extracts of black soybean treated by different high hydrostatic pressure treatments (0.1~150 MPa) and germination (0~4 days) in PC-3 prostate cancer cell

HGSPB1_2018_v31n6_836_t0004.png 이미지

Table. 5. Anti-proliferative effects of crude saponin extracts of black soybean treated by different high hydrostatic pressure treatments (0.1~150 MPa) and germination (0~4 days) in AGS gastric cancer cell

HGSPB1_2018_v31n6_836_t0005.png 이미지

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