Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Fermentative transformation of ginsenosides by a combination of probiotic Lactobacillus helveticus and Pediococcus pentosaceus

프로바이틱스 Lactobacillus helveticus와 Pediococcus pentosaceus의 조합에 의한 진세노사이드의 발효적 형질전환

  • Palaniyandi, Sasikumar Arunachalam (Department of Biotechnology, Mepco Schlenk Engineering College, Mepco Nagar, Mepco Engineering College) ;
  • Le, Bao (Department of Biotechnology, Chonnam National University) ;
  • Kim, Jin-Man (Department of Biotechnology, Chonnam National University) ;
  • Yang, Seung Hwan (Department of Biotechnology, Chonnam National University)
  • ;
  • ;
  • 김진만 (전남대학교 공학대학 생명산업공학과) ;
  • 양승환 (전남대학교 공학대학 생명산업공학과)
  • Received : 2018.07.23
  • Accepted : 2018.10.10
  • Published : 2018.12.31
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Abstract

Ginseng are native traditional herbs, which exhibit excellent pharmacological activities. Probiotic Lactobacillus helveticus KII13 and Pediococcus pentosaceus strain KID7 were used for ginsenoside transformation by fermenting crude ginseng extract to enhance minor gisenoside content. Thin-layer chromatography (TLC) analysis of fermented ginseng extract showed that the minor ginsenosides Rg3, Rh1, and Rh2 were main products after 5 days of fermentation. HPLC analysis was performed to quantify the major and minor ginsenosides. The Rg3 peak appeared on the 3rd day while the appearance of Rh2 peak and Rh1 peak were observed on the 5th day. The co-culture of L. helveticus KII13 and P. pentosaceus KID7 converted major ginsenosides (Rb1 and Rg1) into minor ginsenosides (Rg3, Rh2, and Rh1).

인삼은 우수한 약리 활성 작용을 보이는 전통적인 약초이다. 본 연구에는 프로바이오틱스 Lactobacillus helveticus KII13과 Pediococcus pentosaceus KID7 균주를 진세노사이드(ginsenoside) 함량을 증가시키기 위해 조 인삼 추출물을 발효시켜 진세노사이드를 형질전환 시키는데 사용되었다. 발효삼 추출물의 TLC(Thin-layer chromatography) 분석 결과, 5일간의 발효 후 주요 사포닌인 진세노사이드 Rg3, Rh1 및 Rh2로 변환되는 것으로 나타났다. HPLC 분석을 수행하여 주요 및 미량 진 세노사이드를 정량화하였다. 3일째에는 Rg3가 나타나고, 5일째에는 Rh2 및 Rh1이 나타난다. L. helveticus KII13과 P. pentosaceus KID7의 공동 배양은 주요 진세노사이드(Rb1과 Rg1)를 미량 진세노사이드(Rg3, Rh2, Rh1)로 전환시키는 것을 학인하였다.

Keywords

MSMHBQ_2018_v54n4_436_f0001.png 이미지

Fig. 1. TLC analysis of the fermented ginseng extract.

MSMHBQ_2018_v54n4_436_f0002.png 이미지

Fig. 2. Time course HPLC analysis of the composition of major and minor ginsenoside in ginseng extract fermented by the probiotic strains combination.

MSMHBQ_2018_v54n4_436_f0003.png 이미지

Fig. 3. Schematic representation of the possible hydrolytic pathway of ginsenoside Rg3, Rh2, and Rh1 production from Rb1 and Rg1.

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