Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Cnestis palala (Lour.) Merr. extract suppresses Propionibacterium acnes-induced inflammation

Propionibacterium acnes에 의해 유도되는 염증반응에서 Cnestis palala (Lour.) Merr. 추출물의 억제효과

  • Shin, Jin Hak (Department of Molecular Bioscience, College of Biomedical Science, Institute of Bioscience & Biotechnology, Kangwon National University) ;
  • Lee, Eun Hye (Department of Molecular Bioscience, College of Biomedical Science, Institute of Bioscience & Biotechnology, Kangwon National University) ;
  • Kim, Seon Sook (Department of Molecular Bioscience, College of Biomedical Science, Institute of Bioscience & Biotechnology, Kangwon National University) ;
  • Sydara, Kongmany (Minstry of Health, Institute of Traditional Medicine) ;
  • Seo, Su Ryeon (Department of Molecular Bioscience, College of Biomedical Science, Institute of Bioscience & Biotechnology, Kangwon National University)
  • 신진학 (강원대학교 분자생명과학과) ;
  • 이은혜 (강원대학교 분자생명과학과) ;
  • 김선숙 (강원대학교 분자생명과학과) ;
  • ;
  • 서수련 (강원대학교 분자생명과학과)
  • Received : 2017.11.27
  • Accepted : 2018.01.25
  • Published : 2018.03.31
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Abstract

Acne is an inflammatory skin disease that occurs in puberty and young people. Propionibacterium acnes (P. acnes) is known to be a major cause of inflammation in acne. P. acnes proliferates within hair follicles blocked by overproduced sebum in the skin, and thereby activates monocytic cells to promote the secretion of pro-inflammatory cytokines. In this study, we investigated the possibility of Cnestis palala (Lour.) Merr. extract to diminish P. acnes-mediated inflammatory responses. We found that C. palala extract significantly attenuated P. acnes-induced pro-inflammatory cytokine expressions, such as $IL-1{\beta}$, IL-6, $TNF-{\alpha}$, iNOS, and COX-2 in mouse macrophage RAW264.7 cells. Moreover, we observed that C. palala extract inhibited $NF-{\kappa}B$ transcriptional activation, which is the major transcription factor of inflammatory cytokine expression. Therefore, it is expected that C. palala extract has a potential as a therapeutic agent or supplement for the treatment P. acnes-induced inflammatory responses.

여드름은 사춘기와 젊은 연령층에서 나타나는 염증성 피부질환이다. Propionibacterium acnes (P. acnes)는 여드름에서 염증을 유발하는 주요한 균으로 알려져 있다. P. acnes 는 피부에서 과잉생산된 피지에 의해 막힌 모낭 내에서 증식하며, 피부 내의 단핵구를 활성화시키고 염증성 사이토카인(pro-inflammatory cytokine)의 분비를 촉진하여 염증반응을 증가시키는 것으로 알려져 있다. 본 논문에서는 Cnestis palala (Lour.) Merr. 추출물이 P. acnes 에 의한 염증반응을 감소시킬 수 있는지 확인하고자 하였다. 마우스 대식세포주인 RAW264.7 에서 C. palala 추출물은 P. acnes 에 의해 증가하는 염증성 사이토카인인 $IL-1{\beta}$의 단백질 발현량과 IL-6, $TNF-{\alpha}$, COX-2 의 mRNA 발현량을 감소시켰다. 또한 염증성 사이토카인 발현의 주요 전사인자(transcription factor)인 $NF-{\kappa}B$ 의 전사 활성 역시 C. palala 추출물에 의해 감소하는 것을 확인하였다. 따라서 C. palala 추출물이 P. acnes 에 의해 발생하는 여드름의 치료제나 그 보조제로 사용될 가능성이 있음을 제안 할 수 있다.

Keywords

References

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