Fig 1. Determination of laccase activity in G. lucidum (Y1, Y2, GN) culture supernatants supplemented different concentrations(2%, 4%) of Eleutherococcus senticosus(ES) and sawdust(SD) supernatants. (A): Zymogram of laccase isoenzymes in culture supernatants of G. lucidum, (B): ELISA assay.
Fig 2. Examination of cellulase activity in G. lucidum (Y1, Y2, GN) culture supernatants supplemented different concentrations(2%, 4%) of Eleutherococcus senticosus(ES) and sawdust(SD) supernatants. (A): cellulase activity by congo-red staining, (B): ELISA assay
Fig 3. Growth rate of Ganoderma lucidum by addition of Eleutherococcus senticosus(ES) and sawdust(SD). (A):Yeongji-1(Y1;ASI-7004), (B):Yeongji-2(Y2;ASI-7071), (C):Antler-type(GN;ASI-7013).
References
- Baldrian P, Valasova V. 2008. Degradation of cellulose by basidiomycetous fungi. FEMS Microbiol Rev 32: 501-521. https://doi.org/10.1111/j.1574-6976.2008.00106.x
- Bushwell JA. 1998. Production of lignocellulolytic enzymes by edible mushrooms and their role in substrate utilization, pp. 1-5. Kuala Lumpur, Icro Unesco University. Malaya.
- Inacio FD, Ferreira RO, de Araujo CAV, Peralta RM, de Souza CGM. 2015. Production of enzymes and biotransformation of orange waste by oyster mushroom, Pleurotus pulmonarius (Fr.) Quel. Adv Microbiol 5: 1-8. https://doi.org/10.4236/aim.2015.51001
- Kim JW, Kim HI, Kim JH, Kwon OC, Son ES, Lee CS, Park YJ. 2016. Effects of ganodermanondiol, a new melanogenesis inhibitor from the medicinal mushroom Ganoderma lucidum. Int J Mol Sci 17: 1798. https://doi.org/10.3390/ijms17111798
- Kwon OC, Park YJ, Kim HI, Kong WS, Cho JH, Lee CS. 2016. Taxonomic position and species identity of the cultivated yeongji 'Ganoderma lucidum' in korea. mycobiology 44: 1-6. https://doi.org/10.5941/MYCO.2016.44.1.1
- Li G, Liu X, Yuan L. 2017. Improved laccase production by funalia trogii in ansorbent fermentation with nutrient carrier. J Biosci Bioeng 124: 381-385. https://doi.org/10.1016/j.jbiosc.2017.05.002
- Lim SH, Kang HW. 2016. Industrial applications and characteristics of lignocellulolytic enzymes in Basidiomycetous fungi. J mushroom sci 14: 51-58. https://doi.org/10.14480/JM.2016.14.2.51
- Liu X, Yuan JP, Chung CK, Chen XJ. 2002. Antitumor activity of the sporoderm-broken germinating spores of Ganoderma lucidum. Cancer Lett 182: 155-161. https://doi.org/10.1016/S0304-3835(02)00080-0
- Moncalvo JM. 2000. Systematics of ganoderma, pp. 23-45, In Flood J, Bridge PD, Holderness M(ed.), Ganoderma diseases of perennial crops, CABI Press. Wallingford United kingdom.
- Otjen L, Blanchette R, Effland M, Leatham G. 1987. Assessment of 30 white rot basidiomycetes for selective lignin degradation. Holzforschung 41: 343-349. https://doi.org/10.1515/hfsg.1987.41.6.343
- Park YJ, Nam JY, Yoon DE, Kwon OC, Kim HI, Yoo YB, Kong WS, Lee CS. 2013. Comparison of anti-inflammatory, antioxidant and anti-allergic effects of Ganoderma species mycelial extracts. J mushroom sci 11: 111-115. https://doi.org/10.14480/JM.2013.11.2.111
- Park YJ, Yoon DE, Kim HI, Kwon OC, Yoo YB, Kong WS, Lee CS. 2014. Overproduction of laccase by the white-rot fungus Pleurotus ostreatus using apple pomace as inducer. mycobiology 42: 193-197. https://doi.org/10.5941/MYCO.2014.42.2.193
- Wasser SP, Weis AL. 1999. Medicinal properties of substances occurring in higher basidiomycete mushrooms: current perspectives (Review). Int J Med Mushrooms 1: 31-62. https://doi.org/10.1615/IntJMedMushrooms.v1.i1.30