Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Identification and Genotypic Characterization of Potentially Pathogenic Acanthamoeba Isolated from Tap Water in Wuxi, China

  • Wang, Meixu (Department of Public Health and Preventive Medicine, Wuxi School of Medicine, Jiangnan University) ;
  • Sun, Guangxu (Department of Public Health and Preventive Medicine, Wuxi School of Medicine, Jiangnan University) ;
  • Sun, Yangkai (Department of Public Health and Preventive Medicine, Wuxi School of Medicine, Jiangnan University) ;
  • You, Xiaomin (Department of Public Health and Preventive Medicine, Wuxi School of Medicine, Jiangnan University) ;
  • Li, Xiaoxue (Department of Public Health and Preventive Medicine, Wuxi School of Medicine, Jiangnan University) ;
  • Cheng, Yang (Department of Public Health and Preventive Medicine, Wuxi School of Medicine, Jiangnan University) ;
  • Xuan, Yinghua (Department of Public Health and Preventive Medicine, Wuxi School of Medicine, Jiangnan University)
  • Received : 2018.06.03
  • Accepted : 2018.11.05
  • Published : 2018.12.31
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Abstract

Members of genus Acanthamoeba are widely distributed in the environment. Some are pathogenic and cause keratitis and fatal granulomatous amoebic encephalitis. In this study, we isolated an Acanthamoeba CJW/W1 strain from tap water in Wuxi, Jiangsu Province, China. Its 18S rDNA was sequenced and a phylogenetic tree was constructed. The isolated cysts belonged to morphologic group II. Comparison of 18S rDNA sequences of CJW/W1 strain and other isolates showed high similarity (99.7%) to a clinical isolate Asp, KA/E28. A phylogeny analysis confirmed this isolate belonged to the pathogenic genotype T4, the most common strain associated with Acanthamoeba-related diseases. This is the first report of an Acanthamoeba strain isolated from tap water in Wuxi, China. Acanthamoeba could be a public health threat to the contact lens wearers and, therefore, its prevalence should be monitored.

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References

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