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http://dx.doi.org/10.3347/kjp.2018.56.6.615

Identification and Genotypic Characterization of Potentially Pathogenic Acanthamoeba Isolated from Tap Water in Wuxi, China  

Wang, Meixu (Department of Public Health and Preventive Medicine, Wuxi School of Medicine, Jiangnan University)
Sun, Guangxu (Department of Public Health and Preventive Medicine, Wuxi School of Medicine, Jiangnan University)
Sun, Yangkai (Department of Public Health and Preventive Medicine, Wuxi School of Medicine, Jiangnan University)
You, Xiaomin (Department of Public Health and Preventive Medicine, Wuxi School of Medicine, Jiangnan University)
Li, Xiaoxue (Department of Public Health and Preventive Medicine, Wuxi School of Medicine, Jiangnan University)
Cheng, Yang (Department of Public Health and Preventive Medicine, Wuxi School of Medicine, Jiangnan University)
Xuan, Yinghua (Department of Public Health and Preventive Medicine, Wuxi School of Medicine, Jiangnan University)
Publication Information
Parasites, Hosts and Diseases / v.56, no.6, 2018 , pp. 615-618 More about this Journal
Abstract
Members of genus Acanthamoeba are widely distributed in the environment. Some are pathogenic and cause keratitis and fatal granulomatous amoebic encephalitis. In this study, we isolated an Acanthamoeba CJW/W1 strain from tap water in Wuxi, Jiangsu Province, China. Its 18S rDNA was sequenced and a phylogenetic tree was constructed. The isolated cysts belonged to morphologic group II. Comparison of 18S rDNA sequences of CJW/W1 strain and other isolates showed high similarity (99.7%) to a clinical isolate Asp, KA/E28. A phylogeny analysis confirmed this isolate belonged to the pathogenic genotype T4, the most common strain associated with Acanthamoeba-related diseases. This is the first report of an Acanthamoeba strain isolated from tap water in Wuxi, China. Acanthamoeba could be a public health threat to the contact lens wearers and, therefore, its prevalence should be monitored.
Keywords
Acanthamoeba; tap water; Wuxi;
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